Duodenal Helicobacter pylori infection differs in cagA genotype between asymptomatic subjects and patients with duodenal ulcers

Hamlet, A.; Thoreson, A.-C.E.; Nilsson, Ola; Svennerholm, Ann Mari; Olbe, L

doi:10.1016/s0016-5085(99)70121-6

Cited by 142 publications

(134 citation statements)

References 42 publications

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“…Although not a strict equivalent to gastric cell lines, Hep-2, HT-29, T-84, Madin-Darby canine kidney cells, or Caco-2 cells have been used consistently to study IL-8 release, H. pylori-host interaction, permeability increase, and polymorpho-nuclear leukocyte migration (42)(43)(44)(45)(46). Moreover, the finding that H. pylori can associate with the duodenal and colonic mucosa (47)(48)(49) identical or close to physiological observations make it relevant to the refined dissection of the complex consequences of H. pylori infection.…”

Section: Discussionmentioning

confidence: 99%

Microaerophilic Conditions Permit to Mimic in VitroEvents Occurring during in Vivo Helicobacter pylori Infection and to Identify Rho/Ras-associated Proteins in Cellular Signaling

Cottet

Corthésy–Theulaz

Spertini

et al. 2002

Journal of Biological Chemistry

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Molecular dissection of the mechanisms underlyingHelicobacter pylori infection suffers from the lack of in vitro systems mimicking in vivo observations. A system was developed whereby human epithelial cells (Caco-2) grown as polarized monolayers and bacteria can communicate with each other under culture conditions optimal for each partner. Caco-2 cells grown on filter supports were inserted in a vertical position into diffusion chambers equilibrated with air and 5% CO 2 at their basolateral surface (aerophilic conditions) and 5% CO 2 , 5% O 2 , 90% N 2 (microaerophilic conditions) in the apical compartment. Remarkably, the epithelial polarized layer was stable under these asymmetric culture conditions for at least 24 h, and the presence of Caco-2 cells was necessary to maintain H. pylori growth. In contrast to previous studies conducted with non-polarized Caco-2 cells and other cell lines kept under aerophilic conditions, we found H. pylori-dependent stimulation of cytokine secretion (MCP-1 (monocyte chemoattractant protein-1), GRO-␣ (growth-regulated oncogene-␣), RANTES (regulated on activation normal T cell expressed and secreted)). This correlated with nuclear translocation of NF-B p50 and p65 subunits. Tyrosine phosphorylation of nine cellular proteins was induced or enhanced; we identified p120RasGAP , p190 RhoGAP , p62dok (downstream of tyrosine kinases), and cortactin as H. pylori-inducible targets. Moreover, reduction of H. pylori urease expression was observed in adherent bacteria as compared with bacteria in suspension. In addition to mimicking several observations seen in the inflamed gastric mucosa, the novel in vitro system was allowed to underscore complex cellular events not seen in classical in vitro analyses of microaerophilic bacteria-epithelial cell cross-talk.

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Section: Discussionmentioning

confidence: 99%

Microaerophilic Conditions Permit to Mimic in VitroEvents Occurring during in Vivo Helicobacter pylori Infection and to Identify Rho/Ras-associated Proteins in Cellular Signaling

Cottet

Corthésy–Theulaz

Spertini

et al. 2002

Journal of Biological Chemistry

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“…For the cagA gene subtypıng, primers CAGA-F and CAGA-R yielded a fragment of 298 bp of the cagA gene were used (13). For analysis of the vacA s region, primers VA1-F, VA1-R, SS2-F, and SS3-F were used (2,23,34).…”

Section: Pcr Analysis Of Genotypes In H Pylori Isolatesmentioning

confidence: 99%

Prevalence of genotypes in Helicobacter pylori isolates from patients in eastern Turkey and the association of these genotypes with clinical outcome

Ozbey¹,

Aygün²

2012

Braz. J. Microbiol.

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There is not much information available regarding the prevalence of the genotypes of Helicobacter pylori isolates in Turkey, particularly in eastern Turkey. The aims of this study were to detect the prevalence of different genotypes of H. pylori in Turkish patients with gastrointestinal complaints and to determine the relationship of these genotypes with clinical outcome and sex. One hundred forty H. pylori isolates were examined for the presence of its genotypes by the PCR. We found that the prevalence of vacAs1, vacAs2, cagA, cagE, iceA1, iceA2 and babA2 genes were 88.6%, 11.4%, 71.4%, 35.7%, 41.4%, 58.6%, and 62.1%, respectively. The most predominant vacA subtype was s1a (81.4%). The most vacA allelic combination detected were vacAs1m1 (65.2%) and s1m2 (53.9%) in patients with peptic ulcer and gastritis, respectively.The only vacAs1 isolate was significantly associated with gastritis and peptic ulcer (p<0.05). The vacAs1a, ml, slml and babA2 genes were significantly associated with peptic ulcer (p<0.05), whereas m2 gene was significantly associated with only gastritis (p<0.05). The difference between sex and genotypes was statistically significant among the cagA, vacAs1, iceA2 and babA2 genes. This study reported for the first time the prevalence of H. pylori genotypes in patients with gastrointestinal complaints in eastern Turkey.Further studies are needed to understand epidemiological importance of the genotypes of H. pylori isolates in this region and the association between the virulence genes and clinical outcome in different regions.

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“…61 There has been a recent study suggesting that the cagA status of H. pylori isolated from the duodenal bulb and from the stomach may dier in patients with duodenal ulceration. 62 This observation has not been con®rmed and at the outset does not seem logical considering that H. pylori from the stomach are continuously shed into the bulb and in most regions of the world cagA-negative H. pylori are extremely uncommon.…”

Section: What Is Still Missing That Might Explain Duodenal Ulcer Disementioning

confidence: 99%

Pathogenesis of duodenal ulcer disease: the rest of the story

Dore

Graham

2000

Best Practice & Research Clinical Gastroenterology

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Duodenal Helicobacter pylori infection differs in cagA genotype between asymptomatic subjects and patients with duodenal ulcers

Cited by 142 publications

References 42 publications

Microaerophilic Conditions Permit to Mimic in VitroEvents Occurring during in Vivo Helicobacter pylori Infection and to Identify Rho/Ras-associated Proteins in Cellular Signaling

Microaerophilic Conditions Permit to Mimic in VitroEvents Occurring during in Vivo Helicobacter pylori Infection and to Identify Rho/Ras-associated Proteins in Cellular Signaling

Prevalence of genotypes in Helicobacter pylori isolates from patients in eastern Turkey and the association of these genotypes with clinical outcome

Pathogenesis of duodenal ulcer disease: the rest of the story

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