Dünnschichtchromatographische trennung mehrerer wirkstoffe aus salben und suppositorien und ihre anschliessende direkte quantitative analyse nach der remissionsmethode

Amin, Monir; Jakobs, U.

doi:10.1016/s0021-9673(00)80956-5

Cited by 10 publications

(1 citation statement)

References 22 publications

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“…To our knowledge, only one TLC method has been reported for the estimation of hydrocortisone acetate in combined dosage forms. In 1977, Amin and Jakobs described a direct TLC quantitative analysis of hydrocortisone acetate from ointments and suppositories [6]. The methods recommended by Polish and United States Pharmacopoeias for hydrocortisone acetate determination in bulk drug are UV-spectrophotometry and HPLC-UV [7, 8].…”

Section: Introductionmentioning

confidence: 99%

Validation of a Thin-Layer Chromatography for the Determination of Hydrocortisone Acetate and Lidocaine in a Pharmaceutical Preparation

Dołowy

Kulpińska-Kucia

Pyka

2014

The Scientific World Journal

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A new specific, precise, accurate, and robust TLC-densitometry has been developed for the simultaneous determination of hydrocortisone acetate and lidocaine hydrochloride in combined pharmaceutical formulation. The chromatographic analysis was carried out using a mobile phase consisting of chloroform + acetone + ammonia (25%) in volume composition 8 : 2 : 0.1 and silica gel 60F254 plates. Densitometric detection was performed in UV at wavelengths 200 nm and 250 nm, respectively, for lidocaine hydrochloride and hydrocortisone acetate. The validation of the proposed method was performed in terms of specificity, linearity, limit of detection (LOD), limit of quantification (LOQ), precision, accuracy, and robustness. The applied TLC procedure is linear in hydrocortisone acetate concentration range of 3.75 ÷ 12.50 μg·spot−1, and from 1.00 ÷ 2.50 μg·spot−1 for lidocaine hydrochloride. The developed method was found to be accurate (the value of the coefficient of variation CV [%] is less than 3%), precise (CV [%] is less than 2%), specific, and robust. LOQ of hydrocortisone acetate is 0.198 μg·spot−1 and LOD is 0.066 μg·spot−1. LOQ and LOD values for lidocaine hydrochloride are 0.270 and 0.090 μg·spot−1, respectively. The assay value of both bioactive substances is consistent with the limits recommended by Pharmacopoeia.

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Section: Introductionmentioning

confidence: 99%

Validation of a Thin-Layer Chromatography for the Determination of Hydrocortisone Acetate and Lidocaine in a Pharmaceutical Preparation

Dołowy

Kulpińska-Kucia

Pyka

2014

The Scientific World Journal

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Quantitative thin-layer chromatographic determination of some prostaglandin-derivatives of the subgroups E2, A2 and B2

Amin

1987

Z. Anal. Chem.

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Simultaneous determination of fluocortolone, fluocortolone caproate and nicotinic acid benzylester in pharmaceutical preparations by TLC

Amin

1987

Z. Anal. Chem.

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Dünnschichtchromatographische trennung mehrerer wirkstoffe aus salben und suppositorien und ihre anschliessende direkte quantitative analyse nach der remissionsmethode

Cited by 10 publications

References 22 publications

Validation of a Thin-Layer Chromatography for the Determination of Hydrocortisone Acetate and Lidocaine in a Pharmaceutical Preparation

Validation of a Thin-Layer Chromatography for the Determination of Hydrocortisone Acetate and Lidocaine in a Pharmaceutical Preparation

Quantitative thin-layer chromatographic determination of some prostaglandin-derivatives of the subgroups E2, A2 and B2

Simultaneous determination of fluocortolone, fluocortolone caproate and nicotinic acid benzylester in pharmaceutical preparations by TLC

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