Dünnschicht-chromatographie

Stahl, Egon; Kaltenbach, U.

doi:10.1016/s0021-9673(01)92868-7

Cited by 401 publications

(71 citation statements)

References 6 publications

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“…and visualization was with the universal spray reagent p-anisaldehyde -sulphuric acid -glacial acetic acid 173 [13] followed by flash-heating at approximately 105ºC on a hot plate. Fractions were pooled according to 174 similarities in observed TLC profiles.…”

Section: Lc-hr-esi-ms and -Ms/ms Analysismentioning

confidence: 99%

Isolation of isomangiferin from honeybush (Cyclopia subternata) using high-speed counter-current chromatography and high-performance liquid chromatography

Beer

Jerz

Joubert

et al. 2009

Journal of Chromatography A

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CitationIsolation of isomangiferin from honeybush (Cyclopia subternata) using high-speed counter-current chromatography and high-performance liquid chromatography. 2009, 1216 (19) Isomangiferin was isolated from Cyclopia subternata using a multi-step process including extraction, 19 liquid-liquid partitioning, high-speed counter-current chromatography (HSCCC) and semi-preparative 20 reversed-phase HPLC. Enrichment of phenolic compounds in a methanol extract of Cyclopia subternata 21 leaves was conducted using liquid-liquid partitioning with ethyl acetate -methanol -water (1:1:2, v/v). 22The enriched fraction was further fractionated using HSCCC with a ternary solvent system consisting of 23 tert-butyl methyl ether -n-butanol -acetonitrile -water (3:1:1:5, v/v). Isomangiferin was isolated by semi-24 preparative reversed-phase HPLC from a fraction containing mostly mangiferin and isomangiferin. The 25 chemical structure of isomangiferin was confirmed by LC high-resolution electrospray ionization MS, as 26 well as one and two dimensional NMR spectroscopy. 27 28 29

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Section: Lc-hr-esi-ms and -Ms/ms Analysismentioning

confidence: 99%

Isolation of isomangiferin from honeybush (Cyclopia subternata) using high-speed counter-current chromatography and high-performance liquid chromatography

Beer

Jerz

Joubert

et al. 2009

Journal of Chromatography A

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“…Individual pigments were identified by their characteristic absorption bands and their concentrations were calculated using appropriate equations (Parsons & Strickland, 1965). Individual pigments were resolved by thin layer chromatography and the spots were identified by their characteristic colours with staining reagents and by their RF values (Stahl, 1967). The activities of photosystems I1 and I, and NADP-photoreduction, were measured as described by Codd & Sallal (1978).…”

Section: R H Al-hasan a N D Othersmentioning

confidence: 99%

Correlative Changes of Growth, Pigmentation and Lipid Composition of Dunaliella salina in Response to Halostress

Al-Hasan¹,

Ghannoum²,

Sallal³

et al. 1987

Microbiology

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The growth rate of Dunaliella salina was optimal in the presence of 2.5 to 5 % (w/v) NaCl and growth continued up to 30% NaC1. The carotene to chlorophyll ratio gradually increased from about 1-1 at 2.5% NaCl to 7.7 at 30% NaCl and the chloroplast showed some degeneration; however, with 30% NaCl, intact thylakoids were still recognized. The total lipid content of the cells decreased with increasing salinity but the major lipid classes in all extracts were monogalactosyldiacylglycerols, digalactosyldiacylglycerols, sulphoquinovosyldiacylglycerols and phosphatidylglycerols. Considerable proportions of diacylglycerotrimethylhomoserines were also present, whereas the proportions of phosphatidylcholines and phosphatidylethanolamines were relatively low. Increasing the salinity of the medium resulted in a large increase in the proportions of constituent linolenic acid (18 : 3) both in total lipids and in the galactolipids and of palmitoleic acid (16 : 1) in phosphatidylglycerols. The results are discussed in view of the probable relation of chloroplast lipids to photosynthetic activity, and the fact that the osmoregulator glycerol is produced via photosynthesis.

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“…The aqueous phase, containing gangliosides, was analyzed by HPTLC, using chloroform/methanol/0.2% aqueous CaCl 2 , 6/4/1, by vol, as the eluting solvent system. Visualization was performed with a p-dimethylaminobenzaldehyde spray reagent (23). Quantification of the endogenous GSL pattern was performed by scanning densitometry, using commercial standards as reference compounds.…”

Section: Methodsmentioning

confidence: 99%

Increased Biosynthesis of Glycosphingolipids in Congenital Disorder of Glycosylation Ia (CDG-Ia) Fibroblasts

SALA

2002

Pediatric Research

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Congenital disorder of glycosylation Ia (CDG-Ia) is an autosomal recessive disease, characterized by the impaired biosynthesis of the N-linked oligosaccharide chains of proteins due to a deficiency of phosphomannomutase (PMM), the enzyme converting mannose-6-phosphate into mannose-1-phosphate. We investigated the consequences of the altered N-linked glycoprotein (GP) biosynthesis on the quantity and quality of glycosphingolipids (GSLs) in fibroblasts of CDG-Ia patients. First, we found that CDG-Ia fibroblasts contain an increased amount of total GSLs when compared with normal fibroblasts. Further, we assessed by metabolic labeling of CDG-Ia fibroblasts with radioactive sugar precursors, including galactose and N-acetylmannosamine, that a diminished biosynthesis of cellular GPs is antagonized by an increased biosynthesis of GSLs. An increased GSL biosynthesis was also observed by means of radiolabeled lipid precursors including sphingosine and lactosylceramide. Notably, also the degradation of GLSs is slowed down in CDG-Ia fibroblasts. Finally, when we labeled normal human fibroblasts and CHO cells with radioactive galactose in the presence and absence of deoxymannojirimycin (dMM), an inhibitor of N-glycan processing, we found that this cellular model mimics what occurs in CDG-Ia fibroblasts. Since an inverse relationship between GP expression and GSL content does exist, we assume that increased glycosphingolipid biosynthesis is secondary to protein hypoglycosylation. Altogether, our data suggest that the cell metabolic machinery may be able to partially re-equilibrate protein hypoglycosylation with increased biosynthesis of glycosphingolipids, possibly to preserve the overall physico-chemical equilibrium of the outer layer of the plasma membrane. Glycoproteins (GPs) and glycosphingolipids (GSLs) represent two major classes of glycoconjugates present in mammalian cells. The glycosylation of both classes of molecules shares some common features. Noteworthy, 1) the subcellular distribution of GP and GSL glycosylating enzymes is similar, with the Golgi apparatus being the compartment where the majority of glycosylations occurs (1-3); 2) some of the enzymes which glycosylate proteins and lipids are the same, e.g. those responsible for blood group antigenicity (4, 5); 3) the precursor pool of nucleotide-sugars of both GP and GSL glycosylation within the Golgi lumen is indistinguishable (6); 4) the GP and GSL transport through the Golgi complex displays biochemical and kinetical similarities (7); 5) a relevant portion of neosynthesized GPs and the totality of GSLs are delivered from the Golgi to the plasma membrane, the final site

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Dünnschicht-chromatographie

Cited by 401 publications

References 6 publications

Isolation of isomangiferin from honeybush (Cyclopia subternata) using high-speed counter-current chromatography and high-performance liquid chromatography

Isolation of isomangiferin from honeybush (Cyclopia subternata) using high-speed counter-current chromatography and high-performance liquid chromatography

Correlative Changes of Growth, Pigmentation and Lipid Composition of Dunaliella salina in Response to Halostress

Increased Biosynthesis of Glycosphingolipids in Congenital Disorder of Glycosylation Ia (CDG-Ia) Fibroblasts

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