Dumbbell-shaped metallothionein-templated silver nanoclusters with applications in cell imaging and Hg2+ sensing

Hu, Shengqiang; Ye, Baoyu; Yi, Xinyao; Cao, Zhize; Wu, Daohong; Shen, Congcong; Wang, Jianxiu

doi:10.1016/j.talanta.2016.04.055

Cited by 23 publications

(12 citation statements)

References 57 publications

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“…39,40 These particles can interact with light as they have discreet energy levels due to quantum connement, 41 allowing intense light absorption and emission. 42,43 These optical properties along with superior biocompatibility, brightness and photostability have led to their application as uorescent biolabels.…”

Section: 38mentioning

confidence: 99%

Fluorescent silver nanoparticle based highly sensitive immunoassay for early detection of HIV infection

et al. 2017

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For the first time, we have engineered streptavidin labeled fluorescent silver nanoparticles for their application in immunosensing of biomolecules which will significantly increase sensitivity without compromising the specificity. Plasma samples that were HIV Àve showed no interference with detection of HIV-1 p24 antigen. This technology can be used in resource limited settings and easily adopted for the detection of other pathogen antigens.

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Section: 38mentioning

confidence: 99%

Fluorescent silver nanoparticle based highly sensitive immunoassay for early detection of HIV infection

et al. 2017

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“…74 The orientation of the domains was also established by the single X-ray crystal structure reported in 1991 by Robbins et al 75 Thus, the NMR and X-ray data established the well-known two domain, dumbbell structure of mammalian MTs, which have been recently used as a template for Ag nanoclusters. 76 However, the presence of the two domains dominated the spectral interpretation in a way that may have confused the determination of the metal binding mechanism.…”

Section: Introductionmentioning

confidence: 99%

Unravelling the mechanistic details of metal binding to mammalian metallothioneins from stoichiometric, kinetic, and binding affinity data

2018

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Metallothioneins (MTs) are small, cysteine-rich proteins, found throughout Nature. Their ability to bind a number of different metals with a range of stoichiometric ratios means that this protein family is critically important for essential metal (Zn and Cu) homeostasis, metal storage, metal donation to nascent metalloenzymes as well as heavy metal detoxification. With its 20 cysteines, metallothionein is also considered to protect cells against oxidative stress. MT has been studied by a large number of researchers over the last 6 decades using a variety of spectroscopic techniques. The lack of distinguishing chromophores for the multitude of binding sites has made the evaluation of stoichiometric properties for different metals challenging. Initially, only Cd-NMR spectroscopy could provide strong evidence for the proposed cluster formation of Cd-MT. The extraordinary development of electrospray ionization mass spectrometry (ESI-MS), where all coexisting species in solution are observed, revolutionized MT research. Prior to the use of ESI-MS data, a range of "magic numbers" representing metal-to-MT molar ratios were reported from optical spectroscopic studies. The availability of ESI mass spectral data led to (i) the confirmation of cluster formation, (ii) a conceptual understanding of the cooperativity involved in multiple metal binding events, (iii) the presence of domain specificity between regions of the protein and (iv) mechanistic details involving both binding affinities and rate constants. The kinetic experiments identified the presence of multiple individual binding sites, each with a unique rate constant and an analogous binding affinity. The almost linear trend in rate constants as a function of bound As provided a unique insight that became a critical step in the complete understanding of the mechanistic details of the metalation of MT. To fully define the biological function of this sulfur-rich protein it is necessary to determine kinetic rate constants and binding affinities for the essential metals. Recently, Zn competition experiments between both of the isolated fragments (α and β) and the full-length protein (βα-MT 1a) as well as Zn competition between βα-MT 1a and carbonic anhydrase were reported. From these data, the trend in binding affinities and the values of the K of the 7 bimolecular reactions involved in metalation were determined. From the analysis of ESI-MS data for Cu binding to βα-MT 1a at different pH-values, a trend in the 20 binding affinities for the complete metalation mechanism was reported. This review details a personal view of the historical development of the determination of stoichiometry for metal binding, the structure of the binding sites, the rates of the metalation reactions and the underlying binding affinities for each metalation step. We have attempted to summarize the experimental developments that led to the publication in May 2017 of the experimental determination of the 20 binding constants for the 20 sequential bimolecular reactions for Cu binding to the 20 Cy...

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“…Cancer remains a main threat to human health. , Early diagnosis and treatment of low levels of cancer cells is key to improve the survival rate. − For detection, high sensitivity is crucial for low concentrations of cancer cells . Various optical, − electrochemical, − magnetic, , mass, , and pressure , signaling methods have been developed. Gas pressure-based bioassays are particularly interesting due to their high sensitivity, cost effectiveness, and portability. − …”

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confidence: 99%

NIR Light-Responsive Hollow Porous Gold Nanospheres for Controllable Pressure-Based Sensing and Photothermal Therapy of Cancer Cells

Tong

Wang

et al. 2019

Anal. Chem.

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Pressure-based signal transduction has attracted recent and extensive attention due to its high sensitivity and simplicity. The most popular way to generate gas pressure relies on catalyst-mediated decomposition of H2O2. Despite its high sensitivity, this method lacks spatial and temporal control of the reaction, and may suffer from variations due to the dead time of mixing. In this work, we report a new reaction using near-infrared (NIR) light to heat hollow porous gold nanospheres (AuNSs) for thermal decomposition of NH4HCO3. Comparisons were made on these two systems especially on controlled pressure production. As an example of application, our light-controlled system was used for the detection of MCF-7 cancer cells by attaching the S2.2 aptamer on the AuNSs. The detection limit was as low as 2 cells/mL. Meanwhile, the heat produced by the AuNSs was used to induce localized hyperthermia at the surface of the cancer cells. This interesting theranostic system provides new insights into pressure-based sensing and may inspire new analytical applications.

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Dumbbell-shaped metallothionein-templated silver nanoclusters with applications in cell imaging and Hg2+ sensing

Cited by 23 publications

References 57 publications

Fluorescent silver nanoparticle based highly sensitive immunoassay for early detection of HIV infection

Fluorescent silver nanoparticle based highly sensitive immunoassay for early detection of HIV infection

Unravelling the mechanistic details of metal binding to mammalian metallothioneins from stoichiometric, kinetic, and binding affinity data

NIR Light-Responsive Hollow Porous Gold Nanospheres for Controllable Pressure-Based Sensing and Photothermal Therapy of Cancer Cells

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