Dumas Nitrogen Determination on Decimilligram Scale

Kirsten, Wolfgang; Grunbaum, Benjamin W.

doi:10.1021/ac60107a039

Cited by 33 publications

(6 citation statements)

References 10 publications

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“…Temporary Filling. Use of the apparatus without a temporary filling (2,5) was then tried. When a new tube filling was used, a low result was generally obtained in the first analysis.…”

Section: Discussionmentioning

confidence: 99%

“…The nickel oxide, Hopcalite, and potassium hydroxide have been described (4). Tank carbon dioxide (9) is treated as described (5). Quartz wool is used to hold the tube fillings.…”

Section: Reagentsmentioning

confidence: 99%

“…Avery accurate method for the semimicrodetermination of nitrogen, based upon pyrolysis of the sample in carbon dioxide at 1150°C. and combustion of the formed gases over nickel oxide, described by Kirsten (4), was applied to the decimilligram scale by Kirsten and Grunbaum (5). In the semimicroprocedure backway sweeping was used (8) in connection with a temporary filling, which was changed for every analysis.…”

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Dumas Microdetermination of Nitrogen

Kirsten¹

1957

Anal. Chem.

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butylammonium hydroxide. Because of the electron-withdrawing effect of the two phenyl groups, dibenzoylmethane would be expected to be the stronger acid. When titrated in acetone with sodium or potassium methoxide, dibenzoylmethane actually is a stronger acid than 2,4-pentanedione.An even more striking effect is in the titration of succinic acid. In water the pKi is 4.2 and pK2 is 5.6. From this, the curve would be expected to have little if any inflection when one hydrogen is neutralized, and a sharp end point when the second hydrogen is neutralized. The curve actually obtained (Figure 9) has an excellent break when one equivalent of base has been added, and only a relatively small break at the second inflection point. There is no visible precipitation at any point in the titration. No explanation is offered at this time, but further study of these effects is planned. LITERATURE CITED(1) Brass, D. B., Wvld, G. E. A., Anal.

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“…Temporary Filling. Use of the apparatus without a temporary filling (2,5) was then tried. When a new tube filling was used, a low result was generally obtained in the first analysis.…”

Section: Discussionmentioning

confidence: 99%

“…The nickel oxide, Hopcalite, and potassium hydroxide have been described (4). Tank carbon dioxide (9) is treated as described (5). Quartz wool is used to hold the tube fillings.…”

Section: Reagentsmentioning

confidence: 99%

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confidence: 99%

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Dumas Microdetermination of Nitrogen

Kirsten¹

1957

Anal. Chem.

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“…Mineral quantitative determination of DOR and GPS was carried out using inductively coupled plasmaoptical emission spectrometry (Varian ICP 720-ES model), and nitrogen and carbon were determined by the Dumas method (Kirsten & Grunbaum, 1955) using a Leco TruSpec CN analyzer.…”

Section: Mineral Composition Of Dor and Gpsmentioning

confidence: 99%

Integrated biovalorization of wine and olive mill by-products to produce enzymes of industrial interest and soil amendments

Reina¹,

Ullrich

García-Romera³

et al. 2016

Span. j. agric. res.

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An integral and affordable strategy for the simultaneous production of lignin-modifying and carbohydrate active enzymes and organic amendment, with the aid of a saprobe fungus was developed by using olive oil and wine extraction by-products. The polyporal fungus Trametes versicolor was cultivated in soy or barley media supplemented with dry olive mill residue (DOR) as well as with grape pomace and stalks (GPS) in solid state fermentation (SSF). This strategy led to a 4-fold increase in the activity of laccase, the principal enzyme produced by SFF, in DOR-soy media as compared to controls. T. versicolor managed to secrete lignin-modifying enzymes in GPS, although no stimulative effect was observed. GPS-barley media turned out to be the appropriate medium to elicit most of the carbohydrate active enzymes. The reuse of exhausted solid by-products as amendments after fermentation was also investigated. The water soluble compound polymerization profile of fermented residues was found to correlate with the effect of phytotoxic depletion. The incubation of DOR and GPS with T. versicolor not only reduced its phytotoxicity but also stimulated the plant growth. This study provides a basis for understanding the stimulation and repression of two groups of enzymes of industrial interest in the presence of different carbon and nitrogen sources from by-products, possible enzyme recovery and the final reuse as soil amendments.

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“…For reference Erucic acid content was determined by gas chromatography after harvest in 2009 and 2010 following a standard protocol resulting in a Spearman's rank correlation coefficient of 0.89 ** towards the NIRS-values. NIRS based Chapter 3 -Mapping of QTL for secondary seed dormancy in a winter oilseed rape doubled haploid population predictions for seed protein content showed a high Spearman rank correlation of 0.93 ** with the N-determined protein contents analysed by the Dumas based combustion method (Jung et al 2003, Kirsten andGrunbaum 1955) using a CN Elemental analyzer, type vario EL by Elementar Analysensysteme GmbH (Donaustraße 7, D-63452 Hanau; http://www.elementar.de) as described in the manual (Anonymous 1992). Factor 5.7 was used to calculate the protein content from the nitrogen content (Kroll et al 2007 and references therein).…”

Section: Other Seed Quality Traitsmentioning

confidence: 99%

Genetic variation and inheritance of secondary seed dormancy in winter oilseed rape (Brassica napus L.)

Schatzki¹

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Dumas Nitrogen Determination on Decimilligram Scale

Cited by 33 publications

References 10 publications

Dumas Microdetermination of Nitrogen

Dumas Microdetermination of Nitrogen

Integrated biovalorization of wine and olive mill by-products to produce enzymes of industrial interest and soil amendments

Genetic variation and inheritance of secondary seed dormancy in winter oilseed rape (Brassica napus L.)

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