Dulbecco’s Modified Eagle Medium is neuroprotective when compared to standard vitrectomy irrigation solution

Schultheiß, Maximilian; Januschowski, Kai; Ruschenburg, Hannah; Schramm, Christian; Schnichels, Sven; Szurman, Peter; Bartz‐Schmidt, Karl Ulrich; Spitzer, Martin S.

doi:10.1007/s00417-012-2255-6

Cited by 8 publications

(4 citation statements)

References 25 publications

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“…Once in the hospital, the retina can be cooled with neuroprotective irrigation solution. 23 Before reaching the hospital, however, noninvasive first-aid measures would be desirable, especially considering the narrow time window which is available until reperfusion of the retina has to be achieved. We suggest that it may be beneficial to cool the affected eye with an ice pack.…”

Section: Discussionmentioning

confidence: 99%

Hypothermia Promotes Survival of Ischemic Retinal Ganglion Cells

Reinhard

Marion

Gustafsson

et al. 2016

Invest. Ophthalmol. Vis. Sci.

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Citation: Reinhard K, Mutter M, Gustafsson E, et al. Hypothermia promotes survival of ischemic retinal ganglion cells. Invest Ophthalmol Vis Sci. 2016;57:658-663. DOI:10.1167/ iovs.15-17751 PURPOSE. Ischemic stroke in retinal arteries leads to death of neural tissue and ultimately to blindness. The retina is known to die within 4 hours after onset of ischemia. It is debated whether hypothermia might increase the time window for medical treatment and thereby the chance of recovering sight. In order to characterize the time course of cell death during ischemia and potential beneficial effects of hypothermia in more detail, we investigated the survival of ganglion cells in ischemic pig and human retina as a function of time and temperature.METHODS. Eyes were obtained from minipigs and from human donors post mortem. Enucleated minipig eyes were stored for defined durations at three different temperatures (378C, 218C, and 48C). In order to assess the viability of the tissue, we measured ganglion cell activity (spiking) with multielectrode arrays. RESULTS.Minipig retinal ganglion cell function was severely compromised after 2 hours of ischemia at body temperature. After 4 hours, ganglion cells did not fire action potentials anymore. However, at 218C, ganglion cell activity was maintained under ischemic conditions for up to 12 hours, and for at least 50 hours at 48C. In postmortem human retina, we recorded ganglion cell activity in retinas received up to 27 hours after death.CONCLUSIONS. Our results demonstrate that hypothermia greatly increases survival of retinal ganglion cells exposed to ischemia. These results might be relevant for the future treatment of retinal ischemia.

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Section: Discussionmentioning

confidence: 99%

Hypothermia Promotes Survival of Ischemic Retinal Ganglion Cells

Reinhard

Marion

Gustafsson

et al. 2016

Invest. Ophthalmol. Vis. Sci.

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“…For visualization, sections were fixed with methanol and to stain nuclei, sections were further incubated in a solution containing 0.2 mg/ml 4 0 ,6diamidino-2-phenylindol (DAPI) for 1 min. Stained sections were embedded in FluorSave (Calbiochem, Germany) and imaged using a fluorescence microscope (Axioplan2 imaging ® , Zeiss, Germany with the Openlab software, Improvision, Germany) [22]. All animal experiments comply with the ARRIVE guidelines and were carried out in accordance with the U.K.…”

Section: Selection Of Best Adhering Np and Evaluation Of Adherence Time On The Corneamentioning

confidence: 99%

DNA nanoparticles for ophthalmic drug delivery

et al. 2018

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“…The CCK-8 results showed that IgY@ACP had relative cell viability above 90% on HOK cells and hDPSCs. Specifically, amino acids and other organic components adsorbed on IgY@ACP from DMEM may improve the biocompatibility of calcium phosphate products ( Schultheiss et al, 2013 ). In addition, IgY is an immune antibody produced by immunized hens and is commonly used in the form of mouthwash or toothpaste in the oral environment ( Nguyen et al, 2011 ; Rahman et al, 2013 ).…”

Section: Discussionmentioning

confidence: 99%

Effects of immunoglobulin Y-loaded amorphous calcium phosphate on dentinal tubules occlusion and antibacterial activity

Yan

Guan

Luo

et al. 2022

Front. Bioeng. Biotechnol.

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Aim: This study aimed to evaluate the effects of immunoglobulin Y (IgY)-loaded amorphous calcium phosphate (ACP) (IgY@ACP) on dentinal tubule occlusion and antibacterial activity.Methodology: IgY@ACP was synthesized based on a biomimetic mineralization strategy. The structure was examined by transmission electron microscopy and Fourier transform infrared spectroscopy. The IgY release property was assessed in vitro. The cell biocompatibility of IgY@ACP was evaluated by CCK-8. The dentin disks were prepared using healthy human molars, and their dentinal tubules were exposed to EDTA. Subsequently, they were randomly selected and treated with or without IgY@ACP for 7 days. The tubule occlusion morphologies and newly formed layers were observed by scanning electron microscopy (SEM) and x-ray diffraction, respectively. To evaluate the acid resistance and abrasion resistance of IgY@ACP, dentin disks that were treated for 1 day were immersed in acid solution or subjected to a toothbrush. The antibacterial effects against Streptococcus mutans (S. mutans) were evaluated by colony-forming unit (CFU) counting, adhesion property assessment, and crystal violet and live/dead bacterial staining. Finally, the occlusion effect was evaluated in rat incisors in vivo. One-way analysis of variance (ANOVA) was performed for statistical analysis. The level of significance was set at 0.05.Results: IgY@ACP presented an amorphous phase with a nanosize (60–80 nm) and sustained release of protein within 48 h. The CCK-8 results showed that IgY@ACP had good biocompatibility. After treatment with IgY@ACP for 1 day, the majority of dentinal tubules were occluded by a 0.3-μm-thick mineralized layer. Seven days later, all dentinal tubules were occluded by mineralization with a thickness of 1.4 μm and a depth of 16 μm. The newly mineralized layer showed hydroxyapatite-like diffraction peaks. In addition, IgY@ACP had good acid and abrasion resistance. After treatment with IgY@ACP, the CFU counting and adhesion rate of S. mutans were significantly reduced, the crystal violet staining was lighter, and the S. mutans staining revealed more dead cells. Most importantly, IgY@ACP had a certain occluding property in rat incisors in vivo.Conclusion: IgY@ACP can effectively occlude dentinal tubules with acid-resistant stability and has prominent anti-S. mutans effects, rendering it a potentially suitable desensitization material in the clinic.

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Dulbecco’s Modified Eagle Medium is neuroprotective when compared to standard vitrectomy irrigation solution

Abstract: DMEM seems to support retinal cells very well and to be strongly protective against excitotoxicity. Therefore, DMEM may be considered as possible neuroprotective irrigation solution for PPV.

Cited by 8 publications

References 25 publications

Hypothermia Promotes Survival of Ischemic Retinal Ganglion Cells

Hypothermia Promotes Survival of Ischemic Retinal Ganglion Cells

DNA nanoparticles for ophthalmic drug delivery

Effects of immunoglobulin Y-loaded amorphous calcium phosphate on dentinal tubules occlusion and antibacterial activity

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