Duhuo Jisheng decoction treatment inhibits the sodium nitroprussiate-induced apoptosis of chondrocytes through the mitochondrial-dependent signaling pathway

Liu, Fayuan; Liu, Guozhong; Liang, Wenna; Ye, Hongzhi; Weng, Xiaping; Lin, Pingdong; Li, Huiting; Chen, Jiashou; Liu, Xianxiang; Li, Xihai

doi:10.3892/ijmm.2014.1962

Cited by 26 publications

(18 citation statements)

References 30 publications

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“…The isolated cells were collected every 2 h and the chondrocytes were cultured in 50 ml culture flasks in 4 ml DMEM containing 10% FBS at 37°C and 5% CO 2 . The culture media were changed every 2 days and the cells were subcultured at 90% confluency (17). Collagen Type II Immunohistochemical Staining was performed to confirm the identity of the chondrocytes (data not shown), as previously described from our team (3,17).…”

Section: Methodsmentioning

confidence: 99%

Cibotium barometz polysaccharides stimulate chondrocyte proliferation in vitro by promoting G1/S cell cycle transition

Zheng

Lin

et al. 2017

Molecular Medicine Reports

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Cibotium barometz polysaccharides (CBPS) are one of the most important bioactive components extracted from the Cibotium barometz plant, which belongs to the Dicksoniaceae family. It has been widely used for the treatment of orthopedic diseases in traditional Chinese medicine. However, the molecular mechanisms behind the therapeutic effects of CBPS remain to be clarified. In the present study, the concentration of CBPS was detected by phenol-vitriol colorimetry. Furthermore, the effects stimulated by CBPS on the viability and G1/S cell cycle transition in primary chondrocytes from Sprague-Dawley rats were investigated. A cell viability assay demonstrated that chondrocyte proliferation may be enhanced by CBPS in a dose- and time-dependent manner. The mechanism underlying the promotion of chondrocyte cell cycle was suggested to involve the stimulation of G1 to S phase transition. To further confirm the results, reverse transcription-quantitative polymerase chain reaction and western blot analyses were used to detect the expression of mRNA and protein levels of cyclin D1, cyclin-dependent kinase 4 and retinoblastoma protein. The results suggested that CBPS may stimulate chondrocyte proliferation via promoting G1/S cell cycle transition. Since osteoarthritis is characterized by deficient proliferation in chondrocytes, the present study indicates that CBPS may potentially serve as a novel method for the treatment of osteoarthritis.

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Section: Methodsmentioning

confidence: 99%

Cibotium barometz polysaccharides stimulate chondrocyte proliferation in vitro by promoting G1/S cell cycle transition

Zheng

Lin

et al. 2017

Molecular Medicine Reports

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“…As shown in Figure 7, DHJSD has an overall effect to eliminate "fenghan-shi", respectively, and thus to relieve pain of "Bi Zheng" through promoting "Gan" and "Shen" function and therefore strengthen the bones and tendons [30] . It also improves circulation and remove stasis of "Qi" and "Xue", reduces tissue swelling and relieves pain [20,22,23,26,32,39,40] . Some pharmacological studies have demonstrated that DHJSD may have effects of analgesia, immune function regulation, blood vessel dilation, anti-inflammation, and antiplatelet aggregation [20,22,23,26,32,39,40] .…”

Section: Discussionmentioning

confidence: 99%

“…It also improves circulation and remove stasis of "Qi" and "Xue", reduces tissue swelling and relieves pain [20,22,23,26,32,39,40] . Some pharmacological studies have demonstrated that DHJSD may have effects of analgesia, immune function regulation, blood vessel dilation, anti-inflammation, and antiplatelet aggregation [20,22,23,26,32,39,40] . In a study of the aging-related osteoarthritis, another common bone degenerative disease, Chen et al found that DHJSD may inhibit VEGF and HIF-1α to retard cartilage degradation in a rabbit model of osteoarthritis [53] .…”

Section: Discussionmentioning

confidence: 99%

“…The main function of SDF-1 is to guide cell migration and to further stimulate the chemotaxis of inflammatory cells and induce cell surface to produce integrins. Our previous studies have shown that the SDF-1 expression and apoptosis in degeneration of human (Poria Cocos), Chuanxiong (Rhizoma Chuanxiong), Danggui (Radix Angelicae Sinensis), Niuxi (Radix Achyranthis Bidentatae), Dihuang (Radix Rehmanniae Preparata), Baishao (Radix Paeoniae Alba), Duzhong (Cortex Eucommiae Ulmoidis), Renshen (Panax ginseng) and Gancao (Radix Glycyrrhizae) [20,22,23,26,39,40] , were mixed and soaked in distilled water, rotary evaporator for rotary steam and the solution was filtered and concentrated [41] . After boiled for 30 min twice the DHJSD solution was filtered (filter 0.22 µm) and then dissolved in DMEM/F12 medium containing 15% fetal bovine serum at a final concentration of 10 mg/mL.…”

Section: Introductionmentioning

confidence: 99%

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Duhuo Jisheng Decoction inhibits SDF-1-induced inflammation and matrix degradation in human degenerative nucleus pulposus cells in vitro through the CXCR4/NF-κB pathway

et al. 2018

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Lower back pain (LBP) is the most common disease in orthopedic clinics world-wide. A classic Fangji of traditional Chinese medicine, Duhuo Jisheng Decoction (DHJSD), has been proven clinically effective for LBP but its therapeutic mechanisms remain unclear. We hypothesized that DHJSD might relieve LBP through inhibiting the exaggerated proinflammatory cytokines and extracellular matrix (ECM) degradation. Thus, we studied the effects of DHJSD on stromal cell-derived factor-1 (SDF-1)-induced inflammation and ECM degradation in human nucleus pulposus cells (hNPCs). The primary hNPCs were isolated from either degenerated human intervertebral disc (HID) of LBP patients or normal HID of lumbar vertebral fracture patients, and cultured in vitro. The cells were treated with SDF-1 (10 ng/mL) and subsequently with different concentrations (100-500 μg/mL) of DHJSD for 24 h, respectively. We found that application of DHJSD significantly antagonized the SDF-1-induced production of proinflammatory cytokines and reduction of aggrecan and type II collagen in the hNPCs. DHJSD also markedly reduced the SDF-1-induced increase of CXCR4 and p-p65 and inhibited the nuclear translocation of p65 in the hNPCs. DHJSD, CXCR4-siRNA, and NF-κB inhibitor (BAY11-7082) caused the same inhibition of exaggerated proinflammatory cytokines in the SDF-1-treated hNPCs. These results provided compelling evidence that DHJSD may inhibit the generation of proinflammatory mediators and ECM degradation of HID through an orchestrated targeting at multiple molecules in the SDF-1/CXCR4/NF-κB pathway, thus offered novel mechanistic insights into the clinical effectiveness of DHJSD on LBP.

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“…Previous studies have proven that DHJSD has potential cooperation and polypharmacology against OA, and could inhibit chondrocyte apoptosis by the mitochondria-dependent signaling pathway (16). However, the mechanisms of how DHJSD inhibit the chondrocyte apoptosis by ER stress remain to be elucidated, which has limited its wider use.…”

Section: Introductionmentioning

confidence: 97%

Duhuo Jisheng decoction inhibits endoplasmic reticulum stress in chondrocytes induced by tunicamycin through the downregulation of miR-34a

Liu

Weng

Lin

et al. 2015

International Journal of Molecular Medicine

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Abstract. Our previous study showed that Duhuo Jisheng decoction (DHJSD) inhibited chondrocyte apoptosis by the mitochondria-dependent signaling pathway. Endoplasmic reticulum (ER) stress is upstream of the mitochondria-dependent signaling pathway and has been shown to promote chondrocyte apoptosis that occurs in osteoarthritis (OA). The present study aimed to evaluate whether DHJSD inhibits the chondrocyte apoptosis by regulating ER stress. DHJSD enhanced the viability of tunicamycin (TM)-exposed chondrocytes, a model of ER stress-induced apoptosis, in a dose-and time-dependent manner, as shown by MTT assay. The present results showed that DHJSD and sodium 4-phenylbutyrate (PBA), an ER stress inhibitor, reduced TM-induced chondrocyte apoptosis by 4' ,6-diamidino-2-phenylindole staining. To gain insight into the mechanisms of DHJSD that are responsible for enhancing the viability and inhibiting TM-induced chondrocyte apoptosis, the associated mRNA expressions and protein levels were detected by reverse transcription-polymerase chain reaction (RT-PCR) and western blot analysis, respectively. The results showed that the expression levels of Xbp1, Xbp1s and Bcl-2 were increased, and the expression levels of Bip, Atf4, Chop, Bax, caspase-9 and -3 were decreased in the TM-exposed chondrocytes treated with DHJSD or PBA compared with that in the TM-exposed chondrocytes. To identify the possible mechanisms, the expression of miR-34a was examined by the TaqMan microRNA assay, and was downregulated in the TM-exposed chondrocytes treated with DHJSD or PBA compared with that in the TM-exposed chondrocytes. DHJSD inhibits ER stress in chondrocytes induced by exposure to TM by downregulating miR-34a, suggesting that DHJSD may be a potential therapeutic agent for OA.

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Duhuo Jisheng decoction treatment inhibits the sodium nitroprussiate-induced apoptosis of chondrocytes through the mitochondrial-dependent signaling pathway

Cited by 26 publications

References 30 publications

Cibotium barometz polysaccharides stimulate chondrocyte proliferation in vitro by promoting G1/S cell cycle transition

Cibotium barometz polysaccharides stimulate chondrocyte proliferation in vitro by promoting G1/S cell cycle transition

Duhuo Jisheng Decoction inhibits SDF-1-induced inflammation and matrix degradation in human degenerative nucleus pulposus cells in vitro through the CXCR4/NF-κB pathway

Duhuo Jisheng decoction inhibits endoplasmic reticulum stress in chondrocytes induced by tunicamycin through the downregulation of miR-34a

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