A strongly layered garnet pyroxenite, consisting of cm-scale layers of garnetite, garnet orthopyroxenite, garnet clinopyroxenite, and websterite, occurs in the Biskupice garnet peridotite, which is located in migmatitic gneiss of the Gföhl Assemblage in the Vysočina District, western Moravia. Major-and trace-element compositions and REE patterns of minerals and layers in the pyroxenite are consistent with origin of the layers by HT-HP crystallization and accumulation of variable proportions of garnet, orthopyroxene, and clinopyroxene. The present compositions of the minerals (pyroperich garnet, low-Al, low-Ca enstatite and low-Al, high-Ca diopside) are the result of extensive subsolidus re-equilibration at ~900 ºC and ~37 kbar, during the Variscan (332 Ma) Orogeny. Neodymium and strontium isotopic values (ε