Duckling short beak and dwarfism syndrome virus infection activates host innate immune response involving both DNA and RNA sensors

Chen, Shilong; Fang, T.H.; Xiao, Shifu; Lin, Fu‐Cheng; Cheng, Xiangdong; Shao, Wei; Zhu, Xiaoli; Chen, Xiuqin; Zheng, Min; Munir, Muhammad; Mei-qing, Huang; Yu, Feifei; Chen, Shaoying

doi:10.1016/j.micpath.2019.103816

Cited by 1 publication

(1 citation statement)

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“…Thereafter, qRT-PCR was performed using the PerfectStart Green qPCR SuperMix (Tranz, Beijing) on the Roche 96 Light Cycler (Roche, US), as previously described. The primer sequences used in this analysis have been described in a previous study ( 27 ). The primer sequences of NDRV σB and DAdV-B2 are shown in Table 1 .…”

Section: Methodsmentioning

confidence: 99%

Duck cGAS inhibits DNA and RNA virus replication by activating IFNs and antiviral ISGs

Lin

Zheng²,

Xiao³

et al. 2023

Front. Immunol.

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Cyclic GMP-AMP Synthase (cGAS) is a pivotal adaptor of the signaling pathways involving the pattern recognition receptors and plays an important role in apoptosis and immune regulation. The cGAS function in mammals has been investigated extensively; however, the function of duck cGAS (du-cGAS) in response to viral infections is still unclear. This study aimed to clone the mallard (Anas platyrhynchos) cGAS homolog to investigate the function of duck cGAS (du-cGAS) in host antiviral innate immunity. The results showed that the open reading frame (ORF) region of the du-cGAS gene was 1296 bp, encoding 432 amino acids (aa) and exhibiting similar functional domains with its chicken counterpart. Knockdown of the endogenous du-cGAS by specific sgRNA strongly increased the replication of DNA viruses, including duck adenovirus B2 (DAdV B2) and duck short beak and dwarfism syndrome virus (SBDSV). However, the knockout did not impair the replication of novel duck reovirus (NDRV), an RNA virus. Furthermore, the mRNA expressions of type I interferon (IFNs) and vital interferon-stimulated genes (ISGs) were remarkably reduced in the du-cGAS knockout DEF cell line. Inversely, du-cGAS overexpression greatly activated the transcription of IFN-α, IFN-β, and vital ISGs, and impaired the replication of DAdV B2, SBDSV, and NDRV in the DEF cell line. Importantly, we found that a deletion of 68 aa in the N terminus didn’t impair the antiviral function of du-cGAS. Overexpressing NTase Core, C-Domain (Mab21), or Zinc-Ribbon domain independently had no antiviral effects. Generally, these results reveal that du-cGAS is a vital component of the innate immune system of ducks, with a universal antiviral activity, and provides a useful strategy for the control of waterfowl viral diseases.

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Section: Methodsmentioning

confidence: 99%