Duck TRIM29 negatively regulates type I IFN production by targeting MAVS

Abstract

The innate immune response is a host defense mechanism that induces type I interferon and proinflammatory cytokines. Tripartite motif (TRIM) family proteins have recently emerged as pivotal regulators of type I interferon production in mammals. Here, we first identified duck TRIM29, which encodes 571 amino acids and shows high sequence homology with other bird TRIM29 proteins. DuTRIM29 inhibited IFN-β and IRF7 promoter activation in a dose-dependent manner and downregulated the mRNA expression of IFN-β, IRF7, Mx and IL-6 mediated by duRIG-I. Moreover, duTRIM29 interacted and colocalized with duMAVS in the cytoplasm. DuTRIM29 interacted with duMAVS via its C-terminal domains. In addition, duTRIM29 inhibited IFN-β and IRF7 promoter activation and significantly downregulated IFN-β and immune-related gene expression mediated by duMAVS in ducks. Furthermore, duTRIM29 induced K29-linked polyubiquitination and degradation of duMAVS to suppress the expression of IFN-β. Overall, our results demonstrate that duTRIM29 negatively regulates type I IFN production by targeting duMAVS in ducks. This study will contribute to a better understanding of the molecular mechanism regulating the innate immune response by TRIM proteins in ducks.

Evolution and expression of the duck TRIM gene repertoire

“…In mammals, RNF135, TRIM14, 25, and 65 bind helicases involved in immune signaling using their PRY-SPRY domains, while the function of TRIM47 is unknown ( 104 ). Duck TRIM29 inhibits signaling pathways downstream of these helicases by catalyzing the addition of K29-linked ubiquitin to the signaling adaptor MAVS, resulting in a decrease in IFN signaling downstream of MAVS ( 50 ). RNF135 has previously been excluded from TRIM repertoires because it does not have the classical RBCC motif, as it is missing the B-box domain.…”

“…In mammals and ducks, TRIM25 catalyzes the addition of polyubiquitin for the activation of retinoic-acid-inducible gene I (RIG-I) in antiviral signaling ( 46 , 49 ). Duck TRIM29 was identified as a negative regulator of the RIG-I signaling pathway ( 50 ). A gene described as TRIM39 in chicken is predominantly expressed in the spleen, but has not yet been functionally characterized ( 51 ).…”

When ASFV Infection Meets the cGAS-STING Signaling Pathway

“…Additional research revealed that PA151R and TBK1 engage in a competitive interaction with the E3 enzyme TNF receptor-related protein TRAF6, leading to the suppression of TRAF6 expression via the apoptosis pathway. This ultimately results in the inhibition of TBK1 phosphorylation and the suppression of ASFV replication [113]. PA151R negatively regulates IFN-I production.…”

Whole-Transcriptome Sequencing Reveals That mRNA and ncRNA Levels Correlate with Pleurotus cornucopiae Color Formation

“…In fungi, ncRNAs, such as lncRNA and micro-RNA-like (milRNA) molecules are involved in cell division, nutrient perception, sexual reproduction, and other processes [13][14][15]. CircRNAs regulate gene expression in diverse biological processes in Ganoderma lucidum [16], while milRNAs can regulate P. cornucopiae fruiting body development [17]; however, there are no reports of investigation of the regulation of fruiting body color by ncRNAs.…”