Duck Tembusu virus infection activates the MKK3/6-p38 MAPK signaling pathway to promote virus replication

Cheng, Yuting; Jiao, Linlin; Chen, Jinying; Chen, Peiyao; Zhou, Fang; Zhang, Jilin; Wang, Mixue; Wu, Qingguo; Cao, Shinuo; Lu, Huipeng; Wu, Zhi; Wang, Anping; Qian, Yingjuan; Zhu, Shanyuan

doi:10.1016/j.vetmic.2023.109951

Cited by 2 publications

(2 citation statements)

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“…It may be that the NLRP3 inflammasome is activated, and then mature IL-1β is produced to cause inflammation in the body. p38 MAPK is one of the MAPK family members, cell differentiation, proliferation, inflammation, and death related, 23 which can mediate the inflammatory response by regulating the expression of a variety of inflammatory cytokines such as IL-1β, IL-6, and TNF-α. 24 In this study, histopathological results showed that a large number of inflammatory cells infiltrated in the bronchi and alveoli after modeling, and several diffuse necrosis foci were observed.…”

Section: Discussionmentioning

confidence: 99%

Maqin Xiaoke Granule Alleviates Acute Bronchitis Induced by Smoking Combined With Streptococcus pneumoniae by Regulating NLRP3/p38 MAPK/Glycerophospholipid Pathway

Men,

Liu,

Yang

et al. 2024

Natural Product Communications

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Objective: To explore the efficacy and mechanism of action of Maqin Xiaoke Granules (MQs) in the treatment of acute bronchitis (AB). Methods: The rat model of AB was established by mixed smoke and Streptococcus pneumoniae nasal infection. The pathological changes of lung tissue and bronchus were observed by hematoxylin-eosin (HE) staining and scored. The mRNA expression levels of NOD-like receptor thermal protein domain associated protein 3 ( NLRP3) and p38 mitogen-activated protein kinase ( p38 MAPK) in lung tissue were detected by real-time fluorescence quantitative polymerase chain reaction, and the expression levels of inflammatory factors (tumor necrosis factor-α [ TNF-α], interleukin-6 [ IL-6], interleukin-1β [ IL-1β], interleukin-10 [ IL-10]) in serum and lung homogenate were detected by enzyme-linked immunosorbent assay. The therapeutic effect of MQ on rats with AB was preliminarily evaluated. Metabolic profiling of rat serum was performed by ultra high-performance liquid chromatography-quadrupole-time of flight-mass spectrometry/mass spectrometry, combining P < .05, variable important in projection > 1, fold change > 1.5 and screening for differential metabolites based on univariate and multivariate statistical analyses, and then analyzing the possible metabolic pathways with the help of MetaboAnalyst 5.0 database, to analyze the mechanism of MQ’s action on the treatment of AB at the metabolic level. Results: MQ significantly ameliorated the pathological injury of bronchial and lung tissues in model rats, reduced the mRNA levels of NLRP3 and p38 MAPK ( P < .05, .01), lowered the levels of TNF-α, IL-6, and IL-1β in serum and lung homogenates ( P < .05, .01), and elevated IL-10 in serum and lung homogenates ( P < .0001, .01) levels, and to regulate abnormal metabolites in rats with AB via the glycerophospholipid metabolic pathway. Conclusion: MQ significantly improved AB induced by mixed smoke combined with Streptococcus pneumoniae, and the mechanism of its induction may be related to the dysregulation of glycerophospholipid metabolism, and this study provides a reference for the rational clinical application of MQ.

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Section: Discussionmentioning

confidence: 99%

Maqin Xiaoke Granule Alleviates Acute Bronchitis Induced by Smoking Combined With Streptococcus pneumoniae by Regulating NLRP3/p38 MAPK/Glycerophospholipid Pathway

Men,

Liu,

Yang

et al. 2024

Natural Product Communications

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“…However, during the continuous circulation, these virus isolates have undergone cluster separation and diverged phylogenetically from the TMUV cluster. Based on the reported full-length genome sequences of TMUV and the ML trees of the E, NS1, and NS5 gene sequences, they are divided into four major phylogenetic In recent years, researchers have conducted extensive studies on DTMUV, making progress in understanding various aspects from the virus's genome structure to its pathogenic mechanisms, transmission routes, and host immune responses [11,28]. The analysis of the virus's genome characteristics and protein structures has helped deepen our understanding of its lifecycle and pathogenic mechanisms, providing important clues for vaccine development and drug therapy.…”

Section: Genomic Characteristicsmentioning

confidence: 99%

Advancements in Research on Duck Tembusu Virus Infections

Cheng,

Wang,

et al. 2024

Viruses

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Duck Tembusu Virus (DTMUV) is a pathogen of the Flaviviridae family that causes infections in poultry, leading to significant economic losses in the duck farming industry in recent years. Ducks infected with this virus exhibit clinical symptoms such as decreased egg production and neurological disorders, along with serious consequences such as ovarian hemorrhage, organ enlargement, and necrosis. Variations in morbidity and mortality rates exist across different age groups of ducks. It is worth noting that DTMUV is not limited to ducks alone; it can also spread to other poultry such as chickens and geese, and antibodies related to DTMUV have even been found in duck farm workers, suggesting a potential risk of zoonotic transmission. This article provides a detailed overview of DTMUV research, delving into its genomic characteristics, vaccines, and the interplay with host immune responses. These in-depth research findings contribute to a more comprehensive understanding of the virus’s transmission mechanism and pathogenic process, offering crucial scientific support for epidemic prevention and control.

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Duck Tembusu virus infection activates the MKK3/6-p38 MAPK signaling pathway to promote virus replication

Cited by 2 publications

References 41 publications

Maqin Xiaoke Granule Alleviates Acute Bronchitis Induced by Smoking Combined With Streptococcus pneumoniae by Regulating NLRP3/p38 MAPK/Glycerophospholipid Pathway

Maqin Xiaoke Granule Alleviates Acute Bronchitis Induced by Smoking Combined With Streptococcus pneumoniae by Regulating NLRP3/p38 MAPK/Glycerophospholipid Pathway

Advancements in Research on Duck Tembusu Virus Infections

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