Duck plague virus infection alter the microbiota composition and intestinal functional activity in Muscovy ducks

A Recombinant Duck Plague Virus Containing the ICP27 Deletion Marker Provides Robust Protection in Ducks

“…There are no clinically authorized genetically engineered vaccinations, such as subunit vaccines, gene deletion vaccines, live vector vaccines, DNA-mediated vaccines, and synthetic peptide vaccines, although they are critical for the elimination of duck plague ( 52 ). Among these, gene deletion vaccines are one type of live attenuated vaccination that is thought to be closest to the pathogen’s mode of action and can more successfully generate humoral and cellular immunity ( 53 ). Here, we obtained a genetically stable vaccine candidate carrying an ICP27 deletion marker by using a reverse genetic manipulation method, and we found it can be quickly distinguished from wild-type virus by PCR, WB, IFA, and RFLP methods.…”

Duck plague virus Us3 regulates the expression of pUL48

“…Duck plague ( DP ) is an acute infectious disease characterized by vascular injury, tissue bleeding, gastrointestinal mucosal injury and intestinal mucosal lesions. It is widespread and has a high mortality rate, which has caused serious economic losses to the duck industry ( Dhama et al, 2017 ; Ruan et al, 2022 ; Lefkowitz et al, 2023 ; Shen et al, 2023 ). DP is caused by duck plague virus ( DPV ), which according to the recent classification of the International Committee for the Classification of Viruses ( ICTV ) is classified in the genus Marek's disease virus and belongs to the alpha-herpesviridae, along with herpes simplex virus type 1 (HSV-1) (Lefkowitz et al, 2018).…”

Pathogenicity studies and molecular characterization of DPV infection in ducklings

“…When inoculated with duck embryos, the main manifestations are death of duck embryos and hemorrhage of embryos body; when inoculated with duck embryo fibroblasts, the transparency will generally become lower after 2 to 3 d the particles will increase, the cytoplasm will be concentrated, and the cells will be gradually smooth ( Liu et al, 2020 ). The virus spreads rapidly and has a high lethality rate, causing significant losses to the waterfowl industry globally ( Kong et al, 2023 ). The infection of ducklings up to 20 d of age caused by the current outbreak differed from that reported in the data ( Liang et al, 2022 ).…”