Duck Hepatitis Virus in Duck Embryo Liver Cell Cultures

Hwang, Jen

doi:10.2307/1588259

Cited by 7 publications

(7 citation statements)

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“…The DHV type II antiserum used had a SN50 of >l/16when assayed in embryonated chicken eggs and the DHV type III antiserum had an SN S0 of >l/32 when assayed in embryonated duck eggs. 1/100,000 1/10,000 < 1/100 < 1/100 DISCUSSION The successful cultivation of DEL cells in which DHV Type I produced plaques was in contrast to the findings of Hwang (1966), who concluded that DEL cells were unsuitable for the propagation of DHV. The cultures he described were predominantly fibroblastic and calf serum was used in the growth and maintenance media.…”

Section: Dhv Type II and Iiimentioning

confidence: 85%

“…In vitro assays for DHV type I have not been available until recently/Although DHV type I had been shown to grow in cell cultures of chicken and duck embryo origin with and without cytopathic changes Starr, 1959,1960;Kaeberle et al, 1961;Fitzgerald et al, 1963;Sazawa et al, 1963;Hwang, 1965Hwang, , 1966Maiboroda, 1972; Akulov et al, 1972) the virus produced was usually assayed in ovo or in vivo. A plaque assay for attenuated DHV type I in primary DEK cells was described by Woolcock et al (1982).…”

Section: Introductionmentioning

confidence: 99%

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An assay for duck hepatitis virus type I in duck embryo liver cells and a comparison with other assays

Woolcock

1986

Avian Pathology

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To cite this article: P.R. Woolcock (1986) INTRODUCTIONIn vitro assays for DHV type I have not been available until recently/Although DHV type I had been shown to grow in cell cultures of chicken and duck embryo origin with and without cytopathic changes Starr, 1959,1960;Kaeberle et al., 1961;Fitzgerald et al., 1963;Sazawa et al., 1963;Hwang, 1965Hwang, , 1966Maiboroda, 1972; Akulov et al., 1972) the virus produced was usually assayed in ovo or in vivo. A plaque assay for attenuated DHV type I in primary DEK cells was described by Woolcock et al. (1982). An in vitro assay for virulent DHV has not been described.

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Section: Dhv Type II and Iiimentioning

confidence: 85%

Section: Introductionmentioning

confidence: 99%

An assay for duck hepatitis virus type I in duck embryo liver cells and a comparison with other assays

Woolcock

1986

Avian Pathology

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“…The study by Davis and Woolcock showed that attenuated DHAV-1 grew in embryo cell cultures of goose, turkey, quail, pheasant, guinea fowl, and chicken origin, while virulent virus strains grew to varying degrees in only guinea fowl, quail, and turkey embryo cells, supporting the view that virulent and attenuated DHAV-1 may exhibit difference in terms of growth property [24]. So far, there is only one report describing the propagation of DHAV-3 in DEL cells, and DHAV-3 behaved similarly to DHAV-1 with respect to induction of CPE in virus-infected cells [39, 40].…”

Section: Introductionmentioning

confidence: 87%

“…In addition, conflicting results were obtained from different researchers. For example, Hwang concluded that duck embryo liver (DEL) cells were unsuitable for the propagation of DHAV-1 [29], whereas Woolcock reported that DHAV-1 was propagated successfully in DEL cells [39]. The study by Davis and Woolcock showed that attenuated DHAV-1 grew in embryo cell cultures of goose, turkey, quail, pheasant, guinea fowl, and chicken origin, while virulent virus strains grew to varying degrees in only guinea fowl, quail, and turkey embryo cells, supporting the view that virulent and attenuated DHAV-1 may exhibit difference in terms of growth property [24].…”

Section: Introductionmentioning

confidence: 99%

Effect of fetal calf serum on propagation of duck hepatitis A virus genotype 3 in duck embryo fibroblast cells

Wang

Liu

et al. 2019

BMC Vet Res

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Background Duck viral hepatitis (DVH) is a highly contagious viral disease affecting ducks. It can be caused by five agents, including duck hepatitis A virus genotypes 1 (DHAV-1), 2 (DHAV-2), and 3 (DHAV-3), as well as duck hepatitis virus 2 and duck hepatitis virus 3. Since 2007, DHAV-3 has been known to be the most prevalent in East and South Asia. So far, the information regarding the propagation of DHAV-3 in cultured cells is limited. In this study, we describe the comparative studies on the growth properties of DHAV-3 in primary duck embryo fibroblast (DEF) cells using two different strains: a virulent strain C-GY and an attenuated strain YDF120. The effect of fetal calf serum (FCS) and chick serum (CS) on DHAV-3 replication and the mechanism of the inhibitory effect conferred by FCS were also investigated. Results Following serial passages, both C-GY and YDF120 failed to produce cytopathic effect and plaques. The combined quantitative real-time PCR and indirect immunofluorescence staining methods showed that the two viruses could be propagated productively in DEF cells. Investigation of the viral growth kinetics revealed that the two viruses replicated in DEF cells with similar efficiencies, while the viral load of the virulent C-GY strain peaked more rapidly when compared with the attenuated YDF120 strain. Neutralization assay and time-of-drug-addition study indicated that FCS displayed inhibitory effect on DHAV-3 replication. Analysis on the mechanism of action of FCS against DHAV-3 demonstrated that the inhibitory effect was reflected at three steps of the DHAV-3 life cycle including adsorption, replication, and release. Conclusions Both virulent and attenuated DAHV-3 strains can establish noncytocidal, productive infections in DEF cells. The virulent strain replicates more rapidly than the attenuated strain in early infection period. FCS has an inhibitory effect on DHAV-3 replication, which may be attributed to action of a non-specific inhibitory factor present in FCS directly on the virus. These findings may provide new insights into the development of potential antiviral agents.

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“…Any substantial or systematic reproduction, redistribution, reselling, loan, sub-licensing, systematic supply, or distribution in any form to anyone is expressly forbidden. Terms & Conditions of access and use can be found at http:// www.tandfonline.com/page/terms-and-conditions Growth of duck hepatitis virus (DHV) in cell cultures of chicken and duck embryo origin has been reported associated with and without cytopathic changes Starr, 1959, 1960;Kaeberle etal, 1961;Fitzgerald efai, 1963;Sazawaerai, 1963;Hwang, 1965Hwang, ,1966Maiboroda, 1972;Akulov etal, 1972). However, the titres of virus produced in cell culture have mostly been determined either by in ovo or in vivo assays emphasising that the cytopathic changes observed were not of sufficient practical value for the development of an in vitro assay.…”

Section: Please Scroll Down For Articlementioning

confidence: 99%