Duality of Gonadotropins in Gnathostomes

Quérat, Bruno; Brossas, Caroline; Géniès, Florence; Salmon, Christian

doi:10.1006/gcen.2001.7715

Cited by 57 publications

(22 citation statements)

References 11 publications

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“…Taken together, these data open the possibility that cfLH may cover gonadotropin-regulated functions that are usually separated, and require the complementary activity of both FSH and LH. Notwithstanding this possibility, phylogenetic considerations make it reasonable to assume that teleost fish, like tetrapod vertebrates, express both an FSH-and an LH-type of gonadotropin (Quérat et al 2001). It may therefore not be surprising that we recently identified the gene encoding the putative cfFSH , and confirmed the presence of its mRNA in the pituitary .…”

Section: Discussionmentioning

confidence: 94%

Both recombinant African catfish LH and FSH are able to activate the African catfish FSH receptor

Vischer¹,

Granneman²,

Linskens³

et al. 2003

Journal of Molecular Endocrinology

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LH and FSH are heterodimeric glycoprotein hormones, composed of a common α-subunit non-covalently associated with a hormone-specific β-subunit. Repeated efforts to isolate catfish FSH (cfFSH) have not been successful and only catfish LH (cfLH) has been purified from catfish pituitaries. Recently, however, we succeeded in cloning the cDNA encoding the putative cfFSHβ; the cDNAs for the α-and β-subunit of cfLH have been cloned before.Here we report the expression of biologically active cfLH and cfFSH in the soil amoeba, Dictyostelium discoideum. The biological activity of the recombinant hormones was analyzed using cell lines transiently expressing either the cfLH receptor or the cfFSH receptor. Moreover, a primary testis tissue culture system served to study the steroidogenic potency of the recombinant hormones.Our results demonstrated that Dictyostelium produced biologically active, recombinant catfish gonadotropins, with recombinant cfLH being almost indistinguishable from its native counterpart, purified from pituitaries. Although recombinant cfFSH has significant effects in the bioassays used in this study, the specific function of native cfFSH in the control of reproduction and its expression patterns are not yet understood.

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Section: Discussionmentioning

confidence: 94%

Both recombinant African catfish LH and FSH are able to activate the African catfish FSH receptor

Vischer¹,

Granneman²,

Linskens³

et al. 2003

Journal of Molecular Endocrinology

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“…These glycoprotein hormones are believed to have evolved from a common ancestral molecule through duplication of the β-subunit genes and subsequent divergence (Dayhoff 1976;Kawauchi and Sower 2006). Two GTHs have been identified in all taxonomic groups of gnathostomes, including actinopterygians (Suzuki et al 1988;Querat et al 2000), sarcopterygians (Querat et al 2004), and chondrichthyans (Querat et al 2001), but not in jawless vertebrates (agnathans). A single GTHβ-like protein has recently been identified in sea lampreys by cDNA cloning .…”

Section: Discussionmentioning

confidence: 98%

Gonadotropin-like and adrenocorticotropin-like cells in the pituitary gland of hagfish, Paramyxine atami; immunohistochemistry in combination with lectin histochemistry

2007

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The pituitary system of the hagfish remains an enigma. The present study has aimed to detect possible adenohypophysial hormones in the pituitary gland of the brown hagfish, Paramyxine atami, by means of immunohistochemistry in combination with lectin histochemistry. Rabbit antisera raised against ovine luteinizing hormone (LH)beta, proopiomelanocortin (POMC)-related peptides, and the growth hormone/prolactin family of tetrapod and fish species were used, and 25 kinds of lectins were tested. Three different types of adenohypophysial cells were revealed in the pituitary of brown hagfish. The first was stained with both anti-ovine LH beta and several D-mannose-binding lectins, such as Lens culinaris agglutinin and Pisum sativum agglutinin. This cell type predominated in the adenohypophysis in adults with developing gonads and thus appeared to be involved in the regulation of gonadal functions. The second was negative for anti-ovine LH beta but was stained with several N-acetylglucosamine-binding lectins, such as wheat germ agglutinin and Lycopersicon esculentum lectin. This cell type exhibited a weak positive reaction with anti-lamprey adrenocorticotropin (ACTH) and thus appeared to be related to POMC-like cells. The second cell type was found in the adenohypophysis regardless of the developmental state of the gonads. The third cell type was negative for both antisera and lectins. Since this cell type was numerous in juveniles and adults without developing gonads, most cells of this type were probably undifferentiated. These findings suggest that GTH and ACTH are major adenohypophysial hormones in the hagfish.

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“…Duality in gonadotropins is already established in elasmobranch fishes (Quérat et al, 2001), which have emerged before the divergence between actinopterygians (i.e. chondrostean and bony fish) and sarcopterygians (i.e.…”

Section: Discussionmentioning

confidence: 99%

Receptor-selective determinants in catfish gonadotropin seat-belt loops

Vischer

Marques

Granneman

et al. 2004

Molecular and Cellular Endocrinology

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Mammalian gonadotropins are highly selective. Charge differences between the Cys 10-11 sequence of FSH␤ and LH␤/CG␤ seat-belt loops determine the ability of these hormones to interact with the LH-R. Selective FSH-R binding is mainly dependent on the presence of an FSH␤-specific sequence between Cys 11-12 of the seat-belt loop. Intriguingly, African catfish LH␤ (cfLH␤) lacks a positively charged Cys 10-11 region and stimulates both catfish LH-R and FSH-R with comparable potencies. Our studies on the promiscuous behaviour of cfLH using chimeric gonadotropins revealed that the Cys 10-11 region of cfLH␤ contains cfLH-R-selective determinants, whereas the Cys 11-12 region of cfLH␤ confers FSH-R-stimulating activity to cfLH. Hence, the location of receptor-selective determinants appeared to be fairly well conserved throughout evolution, despite the low sequence identity between mammalian and catfish seat-belt loops. Moreover, various structure-function differences between gonadotropins are discussed in the context of the different (female) reproductive strategies between mammalian and non-mammalian species that required the divergence to a more specific LH-R-stimulating activity of one of the gonadotropins in mammals.

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Duality of Gonadotropins in Gnathostomes

Cited by 57 publications

References 11 publications

Both recombinant African catfish LH and FSH are able to activate the African catfish FSH receptor

Both recombinant African catfish LH and FSH are able to activate the African catfish FSH receptor

Gonadotropin-like and adrenocorticotropin-like cells in the pituitary gland of hagfish, Paramyxine atami; immunohistochemistry in combination with lectin histochemistry

Receptor-selective determinants in catfish gonadotropin seat-belt loops

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