We study the difference analog of the quotient differential operator from [13].Starting with a space of quasi-exponentials W = α x i p ij (x), i = 1, . . . , n, j = 1, . . . , n i , where α i ∈ C * and p ij (x) are polynomials, we consider the formal conjugate Š † W of the quotient difference operator ŠW satisfying S = ŠW S W . Here, S W is a linear difference operator of order dim W annihilating W , and S is a linear difference operator with constant coefficients depending on α i and deg p ij (x) only. We show that ker Š † W is also a space of quasi-exponentials, describe its basis and discrete exponents.We also consider a similar construction for differential operators associated with spaces of quasi-polynomials, which are linear combinations of functions of the form x z q(x), where z ∈ C and q(x) is a polynomial.Combining our results with the results on the bispectral duality obtained in [6], we relate the construction of the quotient difference operator to the (gl k , gl n )-duality of the trigonometric Gaudin Hamiltonians and trigonometric Dynamical Hamiltonians acting on the space of polynomials in kn anticommuting variables.