Dual‐wield NTPases: A novel protein family mined from AlphaFold DB

Abstract

AlphaFold protein structure database (AlphaFold DB) archives a vast number of predicted models. We conducted systematic data mining against AlphaFold DB and discovered an uncharacterized P‐loop NTPase family. The structure of the protein family was surprisingly novel, showing an atypical topology for P‐loop NTPases, noticeable twofold symmetry, and two pairs of independent putative active sites. Our findings show that structural data mining is a powerful approach to identifying undiscovered protein families.