Dual-wavelength photo-killing of methicillin-resistant Staphylococcus aureus

Leanse, Leon G.; Goh, Xueping Sharon; Cheng, Ji‐Xin; Hooper, David C.

doi:10.1172/jci.insight.134343

Cited by 23 publications

(26 citation statements)

References 32 publications

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“…For example, the combination of blue light at 460 and 405 nm was reported to be effective against blue light-tolerant S. aureus . Leanse et al [ 254 ] revealed that blue light at 460 nm (90–360 J/cm 2 ) inactivated staphyloxanthin (a ROS scavenger; antioxidant) through photolysis and thus disrupted the ability of S. aureus to resist blue light treatment. Subsequent treatment with blue light at 405 nm (90–180 J/cm 2 ) inactivated the bacteria (planktonic or in biofilm) at a higher rate than when single wavelengths were used, albeit inactivation was dependent on the dosage of both 405- and 460-nm blue light.…”

Section: Blue Light Versus Antimicrobial Resistance and Consequencmentioning

confidence: 99%

Antimicrobial Blue Light versus Pathogenic Bacteria: Mechanism, Application in the Food Industry, Hurdle Technologies and Potential Resistance

Hadi

Brightwell

2020

Foods

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Blue light primarily exhibits antimicrobial activity through the activation of endogenous photosensitizers, which leads to the formation of reactive oxygen species that attack components of bacterial cells. Current data show that blue light is innocuous on the skin, but may inflict photo-damage to the eyes. Laboratory measurements indicate that antimicrobial blue light has minimal effects on the sensorial and nutritional properties of foods, although future research using human panels is required to ascertain these findings. Food properties also affect the efficacy of antimicrobial blue light, with attenuation or enhancement of the bactericidal activity observed in the presence of absorptive materials (for example, proteins on meats) or photosensitizers (for example, riboflavin in milk), respectively. Blue light can also be coupled with other treatments, such as polyphenols, essential oils and organic acids. While complete resistance to blue light has not been reported, isolated evidence suggests that bacterial tolerance to blue light may occur over time, especially through gene mutations, although at a slower rate than antibiotic resistance. Future studies can aim at characterizing the amount and type of intracellular photosensitizers across bacterial species and at assessing the oxygen-independent mechanism of blue light—for example, the inactivation of spoilage bacteria in vacuum-packed meats.

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Section: Blue Light Versus Antimicrobial Resistance and Consequencmentioning

confidence: 99%

Antimicrobial Blue Light versus Pathogenic Bacteria: Mechanism, Application in the Food Industry, Hurdle Technologies and Potential Resistance

Hadi

Brightwell

2020

Foods

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“…In this study, the 460 nm light was proposed to inactivate an oxygen quencher staphyloxanthin from the cell surface, which showed a protective action against 405 nm aBL exposure. [30]. Another dual-light approach was conducted by Guffrey et al, wherein the effect of a combination of 405 nm blue light and 880 nm infrared light on S. aureus and Pseudomonas aeruginosa was tested.…”

Section: Discussionmentioning

confidence: 99%

Daily Administered Dual-Light Photodynamic Therapy Provides a Sustained Antibacterial Effect on Staphylococcus aureus

et al. 2021

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New means to reduce excessive antibiotic use are urgently needed. This study tested dual-light aPDT against Staphylococcus aureus biofilm with different relative ratios of light energy with indocyanine green. We applied single-light aPDT (810 nm aPDT, 405 aBL) or dual-light aPDT (simultaneous 810 nm aPDT and 405 nm aBL), in both cases, together with the ICG photosensitizer with constant energy of 100 or 200 J/cm2. Single-dose light exposures were given after one-day, three-day, or six-day biofilm incubations. A repeated daily dose of identical light energy was applied during biofilm incubations for the three- and six-day biofilms. Using 100 J/cm2 light energy against the one-day biofilm, the dual-light aPDT consisting of more than half of aBL was the most effective. On a three-day maturated biofilm, single-dose exposure to aPDT or dual-light aPDT was more effective than aBL alone. With total light energy of 200 J/cm2, all dual-light treatments were effective. Dual-light aPDT improves the bactericidal effect on Staphylococcus aureus biofilm compared to aPDT or aBL and provides a sustained effect. An increase in the relative ratio of aBL strengthens the antibacterial effect, mainly when the treatment is repeatedly applied. Thus, the light components’ energy ratio is essential with dual-light.

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“…It should be noted that the important role of porphyrin and flavin sensitizers in the realization of the biological effect of light in the blue spectral region was previously established for microbial cells (48,100). At the same time, in recent years, the antimicrobial effect of optical radiation in the blue spectral region through photolysis of the membrane-bound carotenoid pigment, staphyloxanthin (101)(102)(103), has been actively studied. The specified pigment is a triterpenoid, C30-carotenoid and is produced by Staphylococcus aureus including methicillin-resistant Staphylococcus aureus (MRSA).…”

Section: •-mentioning

confidence: 99%

“…It is characterized by a wide absorption spectrum in the blue-green region (from 370 to 530 nm) with a maximum at 463 nm and a pronounced shoulder at 490 nm. It is believed (104) that this pigment is an antioxidant (singlet oxygen quencher) and, when exposed to light corresponding to its absorption band, it is prone to photolysis, which directly leads to photoinactivation of MRSA (101)(102)(103). To our knowledge, the possible role of photolysis of pigments with antioxidant properties in the regulatory effect of light on animal sperm has not been discussed in the literature.…”

Section: •-mentioning

confidence: 99%

Comparative Effect of Low‐intensity Laser Radiation in Green and Red Spectral Regions on Functional Characteristics of Sturgeon Sperm

Plavskiĭ

Микулич

Barulin

et al. 2020

Photochem & Photobiology

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A comparative study of the effect of low‐intensity laser radiation in green (λ = 532 nm) and red (λ = 632.8 nm) spectral regions at equal average irradiance (3 mW cm−2) on functional characteristics of Siberian sturgeon spermatozoa is carried out. Confirmation of the photobiomodulation effect of the radiation is obtained by analyzing spermatozoa motility, percentage of motile spermatozoa and fertilization rate. It is shown that, depending on the energy dose, the laser radiation in red and green spectral regions can have both stimulatory and inhibitory effects on spermatozoa motility. Contrary to popular belief that the short‐wavelength radiation has great prospects in reproductive biotechnologies (due to more efficient absorption of radiation by cellular chromophores and increased generation of ROS), convincing evidence of a more pronounced stimulatory effect of radiation in the red spectral region was obtained. For the first time, metal‐free porphyrins capable of acting as endogenous photosensitizers generating ROS were detected and identified in animal sperm. Using luminol‐dependent chemiluminescence, it is shown that the increased production of ROS capable of exerting an inhibitory effect on biological systems at high concentrations is among the possible reasons for reduction in the stimulatory effect of radiation when moving from red to green spectral region.

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Dual-wavelength photo-killing of methicillin-resistant Staphylococcus aureus

Cited by 23 publications

References 32 publications

Antimicrobial Blue Light versus Pathogenic Bacteria: Mechanism, Application in the Food Industry, Hurdle Technologies and Potential Resistance

Antimicrobial Blue Light versus Pathogenic Bacteria: Mechanism, Application in the Food Industry, Hurdle Technologies and Potential Resistance

Daily Administered Dual-Light Photodynamic Therapy Provides a Sustained Antibacterial Effect on Staphylococcus aureus

Comparative Effect of Low‐intensity Laser Radiation in Green and Red Spectral Regions on Functional Characteristics of Sturgeon Sperm

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