Dual-wavelength excitation of mucosal autofluorescence for precise detection of diminutive colonic adenomas

Imaizumi, Katsuichi; Harada, Yoshinori; Wakabayashi, Naoki; Yamaoka, Yoshihisa; Konishi, Hideyuki; Dai, Pengcheng; Tanaka, Hideo; Takamatsu, Tetsuro

doi:10.1016/j.gie.2011.08.012

Cited by 29 publications

(27 citation statements)

References 43 publications

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“…Unlike R27, the 365/405 AF image is reported to have only limited influence from the submucosa which the authors partially attribute to hemoglobin absorption. 26 In this imaging study of surgical specimens we have obtained results indicating that the capability to detect colon neoplasms could be expanded by including both 340 and 440 nm excitation for AF imaging as well as the reflectance bands 480 and 555 nm. These wavelengths incorporate contrast primarily from NADH, submucosal collagen, and hemoglobin.…”

Section: Discussionmentioning

confidence: 98%

“…26 We expect the ratio image R27 to produce better contrast than a 365/405 AF image when imaging in situ polyps because it incorporates contrast from both mucosal NADH (F340) and submucosal collagen (F440). Unlike R27, the 365/405 AF image is reported to have only limited influence from the submucosa which the authors partially attribute to hemoglobin absorption.…”

Section: Discussionmentioning

confidence: 99%

“…25 A third study used AF ratio images to highlight adenomatous regions in cross sections of excised colonic polyps. 26 Still others have demonstrated the use of AF ratio images microscopically for monitoring cell metabolism and differentiating cancerous tissue. [27][28][29] Previously, we reported early results from an AF imaging study of fresh surgical specimens of human colon.…”

Section: Introductionmentioning

confidence: 99%

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Ratio images and ultraviolet C excitation in autofluorescence imaging of neoplasms of the human colon

Renkoski

Banerjee

Graves³

et al. 2013

J. Biomed. Opt

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Abstract. The accepted screening technique for colon cancer is white light endoscopy. While most abnormal growths (lesions) are detected by this method, a significant number are missed during colonoscopy, potentially resulting in advanced disease. Missed lesions are often flat and inconspicuous in color. A prototype ultraviolet spectral imager measuring autofluorescence (AF) and reflectance has been developed and applied in a study of 21 fresh human colon surgical specimens. Six excitation wavelengths from 280 to 440 nm and formulaic ratio imaging were utilized to increase lesion contrast and cause neoplasms to appear bright compared to normal tissue. It was found that in the subset of lesions which were most difficult to visualize in standard color photographs [low contrast lesions, (LCLs)] a ratio image (F340/F440) of AF images excited at 340 and 440 nm produced extraordinary images and was effective in about 70% of these difficult cases. Contrast may be due to increased levels of reduced nicotinamide adenine dinucleotide, increased hemoglobin absorption, and reduced signal from submucosal collagen. A second successful ratio image (R480/R555) combined two reflectance images to produce exceptional images especially in particular LCLs where F340/F440 was ineffective. The newly discovered ratio images can potentially improve detection rate in screening with a novel AF colonoscope.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Ratio images and ultraviolet C excitation in autofluorescence imaging of neoplasms of the human colon

Renkoski

Banerjee

Graves³

et al. 2013

J. Biomed. Opt

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“…These studies showed the fluorescence index to be a useful marker for discriminating lymphomas from benign lymphoid hyperplasias 80 and predicting the dysplastic grade of colon adenomas. 79,81 Endoscopists (Table 3) The evaluation of AFI images is simply based on the intensity of magenta color, regardless of the complex morphological findings. van den Broek et al 66 found moderate interobserver agreement for AFI (c ϭ 0.58) and poor agreement for NBI in nonexperienced endoscopists ( ϭ 0.33), whereas experienced endoscopists had excellent interobserver agreement for NBI ( ϭ 0.77), but fair agreement for AFI ( ϭ 0.33).…”

Section: Characterization (Table 2)mentioning

confidence: 99%

Image-enhanced endoscopy for the diagnosis of colon neoplasms

Fujiya

Kohgo

2013

Gastrointestinal Endoscopy

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“…However, recent studies have identified a need for improved screening effectiveness, showing that current methods are limited in their ability to detect flat 6 or small (<5 mm diameter) lesions. 3,12,13 Two studies suggest that flat adenomas may account for 22% and 36% of adenomas. 14,15 Hence, there is a need for colorectal screening approaches that offer improved sensitivity and specificity, especially for detection of flat and/or small lesions.…”

Section: Introductionmentioning

confidence: 99%

Hyperspectral imaging fluorescence excitation scanning for colon cancer detection

et al. 2016

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Abstract. Optical spectroscopy and hyperspectral imaging have shown the potential to discriminate between cancerous and noncancerous tissue with high sensitivity and specificity. However, to date, these techniques have not been effectively translated to real-time endoscope platforms. Hyperspectral imaging of the fluorescence excitation spectrum represents new technology that may be well suited for endoscopic implementation. However, the feasibility of detecting differences between normal and cancerous mucosa using fluorescence excitation-scanning hyperspectral imaging has not been evaluated. The goal of this study was to evaluate the initial feasibility of using fluorescence excitation-scanning hyperspectral imaging for measuring changes in fluorescence excitation spectrum concurrent with colonic adenocarcinoma using a small pre-pilot-scale sample size. Ex vivo analysis was performed using resected pairs of colorectal adenocarcinoma and normal mucosa. Adenocarcinoma was confirmed by histologic evaluation of hematoxylin and eosin (H&E) permanent sections. Specimens were imaged using a custom hyperspectral imaging fluorescence excitation-scanning microscope system. Results demonstrated consistent spectral differences between normal and cancerous tissues over the fluorescence excitation range of 390 to 450 nm that could be the basis for wavelength-dependent detection of colorectal cancers. Hence, excitation-scanning hyperspectral imaging may offer an alternative approach for discriminating adenocarcinoma from surrounding normal colonic mucosa, but further studies will be required to evaluate the accuracy of this approach using a larger patient cohort.

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Dual-wavelength excitation of mucosal autofluorescence for precise detection of diminutive colonic adenomas

Cited by 29 publications

References 43 publications

Ratio images and ultraviolet C excitation in autofluorescence imaging of neoplasms of the human colon

Ratio images and ultraviolet C excitation in autofluorescence imaging of neoplasms of the human colon

Image-enhanced endoscopy for the diagnosis of colon neoplasms

Hyperspectral imaging fluorescence excitation scanning for colon cancer detection

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