Dual-wavelength common-path digital holographic microscopy for quantitative phase imaging based on lateral shearing interferometry

Abstract

A dual-wavelength common-path digital holographic microscopy based on a single parallel glass plate is presented to achieve quantitative phase imaging, which combines the dual-wavelength technique with lateral shearing interferometry. Two illumination laser beams with different wavelengths (λ₁=532 nm and λ₂=632.8 nm) are reflected by the front and back surfaces of the parallel glass plate to create the lateral shear and form the digital hologram, and then the hologram is reconstructed to obtain the phase distribution with a synthetic wavelength Λ=3339.8 nm. The experimental configuration is very compact, with the advantages of vibration resistance and measurement range extension. The experimental results of the laser-ablated pit, groove, and staircase specimens show the feasibility of the proposed configuration.

Quantitative and Dynamic Phase Imaging of Biological Cells by the Use of the Digital Holographic Microscopy Based on a Beam Displacer Unit

“…As is shown in Fig. 1(b), after the diverging beam traveling through the beam displacer unit, the optical path of the two generated beams are different, resulting in the difference of the wavefront curvature radius [14]. In the experiment, by recording two holograms with and without the specimen in the FOV, the phase aberration can be removed using double-exposure digital holographic interferometry.…”

Quantitative Phase Imaging Using Deep Learning-Based Holographic Microscope

“…After that, the quantitative phase information can be converted to dry mass density of the cell with extremely high accuracy which has been demonstrated so far as a valuable tool in hematological or cancer diagnosis. The label-free, submicron scale sensitivity, full-field, non-destructive, real-time, quantitative and three-dimensional imaging abilities of DHM present a variety of advantages for biomedical applications, especially for live cell imaging [14][15][16]. Nowadays, DHM has been an important and powerful tool for medical diagnoses.…”

Single-Element Reflective Digital Holographic Microscopy

“…For instance, such a beam can be created: (1) by the reflection of the light in a reference mirror inside a Mirau interferometric objective [18]; (2) by a spatial filtering at the Fourier plane in diffraction phase microscopy [17,19]; by using the clear regions near to sparse samples in lateral shearing interferometry [24][25][26][27], (3) by spatially-multiplexing the field of view (FOV) [23], or (4) by using a modulator mask in single pixel phase imaging [21,22].…”

“…Among all CPIs described in the literature, there are some of them that employ just one optical element to produce interferences, such as a Lloyd's mirror [28], a thick glass plate [26], a diffraction grating [29], a beam splitter cube [30], or a Fresnel's biprism [31][32][33][34]. Those reduce even more the price and the complexity of CPIs and present a very simple usability.…”