Dual utility of a novel, copper enhanced laccase from Trichoderma aureoviridae

Khambhaty, Yasmin; Ananth, Swetha; Sreeram, Kalarical Janardhanan; Rao, Jonnalagadda Raghava; Nair, Balachandran Unni

doi:10.1016/j.ijbiomac.2015.07.051

Cited by 19 publications

(10 citation statements)

References 26 publications

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“…xenobiotic and phenolic lignin model compounds Peng et al, 2015). Earlier, its application was limited because of the low oxidation potential, thus, in the presence of an appropriate mediator; laccases show higher oxidation capability resulting in numerous biotechnological applications involving oxidation of non-phenolic lignin compounds and detoxification of various environmental pollutants (Upadhyay et al, 2016;Khambhaty et al, 2015). Recently, laccase has found applications in other sectors, such as in the design of biosensors and nanotechnology (Li et al, 2014;Upadhyay et al, 2016).…”

Section: Mediators Of Laccasementioning

confidence: 99%

Biodegradation of polycyclic aromatic hydrocarbons (PAHs) by fungal enzymes: A review

Kadri

Rouissi

Brar

et al. 2017

Journal of Environmental Sciences

354

118

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Polycyclic aromatic hydrocarbons (PAHs) are a large group of chemicals. They represent an important concern due to their widespread distribution in the environment, their resistance to biodegradation, their potential to bioaccumulate and their harmful effects. Several pilot treatments have been implemented to prevent economic consequences and deterioration of soil and water quality. As a promising option, fungal enzymes are regarded as a powerful choice for degradation of PAHs. Phanerochaete chrysosporium, Pleurotus ostreatus and Bjerkandera adusta are most commonly used for the degradation of such compounds due to their production of ligninolytic enzymes such as lignin peroxidase, manganese peroxidase and laccase. The rate of biodegradation depends on many culture conditions, such as temperature, oxygen, accessibility of nutrients and agitated or shallow culture. Moreover, the addition of biosurfactants can strongly modify the enzyme activity. The removal of PAHs is dependent on the ionization potential. The study of the kinetics is not completely comprehended, and it becomes more challenging when fungi are applied for bioremediation. Degradation studies in soil are much more complicated than liquid cultures because of the heterogeneity of soil, thus, many factors should be considered when studying soil bioremediation, such as desorption and bioavailability of PAHs. Different degradation pathways can be suggested. The peroxidases are heme-containing enzymes having common catalytic cycles. One molecule of hydrogen peroxide oxidizes the resting enzyme withdrawing two electrons. Subsequently, the peroxidase is reduced back in two steps of one electron oxidation. Laccases are copper-containing oxidases. They reduce molecular oxygen to water and oxidize phenolic compounds.

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Section: Mediators Of Laccasementioning

confidence: 99%

Biodegradation of polycyclic aromatic hydrocarbons (PAHs) by fungal enzymes: A review

Kadri

Rouissi

Brar

et al. 2017

Journal of Environmental Sciences

354

118

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“…Media optimization and use of appropriate inducers could bring additional benefits of higher production with expenditure of minimum resources. Understanding a better mode of cultivation using mediators, possibly extend the role of laccase to non-phenolic substrates [30]. So far, this is the first report demonstrating the decolorizing activity in the Cercospora genre.…”

Section: Discussionmentioning

confidence: 83%

Comparative Study of Dye Decolorization using free and Alginate Gel Entrapped Laccase from Cercospora sp. SPF-6

Chand¹

2018

AIBM

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Laccase have a promising potential to be used as a bio-remediating agent that can be used to solve various environmental issues. In this study, extracellular laccase enzyme was produced from Cercospora sp SPF-6 isolated from the soils of Himachal Pradesh and was immobilized by entrapment in calcium alginate beads with immobilization efficiency of 72.42%and enzyme activity of 0.617 U/gel was assessed for its dye decolorization potential. Free laccase showed maximum decolorization of methyl orange dye at 35 ˚C in 90 min with citrate phosphate buffer (0.1 M, pH 3.0) as compared to immobilized enzyme at 25 ˚C with the same buffer (0.025M, pH 2.5) in 30 min. At 50 ml flask scale with same optimized conditions, the free enzyme in fed batch showed 99.4% decolorization compared to batch and with immobilized enzyme both batch and fed batch showed approximately 77% dye decolorization. In the BRUNSWICK fermenter (500 ml), with immobilized enzyme, batch and fed batch showed 68% and 72% decolorization respectively (1.36IU x 6,000 beads). Immobilized enzyme showed reusability upto 7th reaction cycle with 50% decolorization efficiency. These preliminary results suggested that immobilized laccase in alginate beads can be potentially developed for large scale bioremediation of other toxic dyes with bioreactors.

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“…Trichoderma spp are well known for their ability to grow on decayed wood and other agrolignocellulosic biomass materials and can decompose lignin by secreting enzymes such as cellulase and laccases. Based on this, and the fact that Trichoderma spp can produce high amount of laccase in liquid cultures, laccases from this fungus may promote degradation/modification of lignin or ligninderived components [14,15] and therefore represent proper candidates in processing lignocellulosic biomass.…”

Section: Introductionmentioning

confidence: 99%

Screening of Laccase Producing Fungi Using Agro-Wastes under Different Cultural Conditions

Ndochinwa

Amadi

Nwagu

et al. 2020

JABB

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This study evaluated the potential of fungal isolates for laccase production. Fungi cultures were screened for laccase production on plate assay using 2’ 2’ – azinobis-(-3-ethyl benzthiazoline -6- suphonate) (ABTS), and by submerged fermentation. Result obtained from the plate assay showed the formation of green halo after 2-4 days of incubation due to oxidation of 2’ 2’ – azinobis-(-3-ethyl benzthiazoline -6- suphonate) (ABTS) which is as a result of lignolytic enzymes production Utilization of selected agro-wastes residues (sawdust, plantain and banana peels) for laccase production was evaluated. Fungal isolates were identified based on their cultural characteristics according to standard mycology methods. Light microscopy was performed on cultures and the fungal isolates were identified by their different morphological and colonial characteristics after which they were confirmed using fungal atlas. Total protein content was also determined using the Bradford method Five out of twelve isolates were positive for oxidation of 2’ 2’ – azinobis-(-3-ethyl benzthiazoline -6- suphonate) (ABTS) which signifies laccase enzyme activity and identified as Geotrichum spp, Cephalosporium spp, Trichoderma spp, Trametes spp and Fusarium sppThe highest enzyme activity was observed using Trichoderma spp at 57.1U/l, Trametes spp 51.99 U/l, Fusarium spp had 29.2 U/l. Geotrichum spp and Cephalosporium spp were least at 28.04 U/l and 9.72 U/l respectively. Trichoderma spp was used for further studies. Effect of carbon sources, inoculum size, pH, total protein and shaker speed on laccase production was evaluated. Sawdust gave the highest yield of laccase enzyme 151.17U /l, followed by plantain peel with 62.49U/l of enzyme, banana peel recorded the least of laccase enzyme 54.94 U /l. Protein content obtained from sawdust medium was 45.8mg/ml, plantain peel medium had 29.2 mg/ml while banana peel was the lowest with 16.8mg/ml all at the sixth day of the fermentation process. Optimum pH for sawdust, banana peel and plantain peel was 5.95, 5.94, and 5.83 respectively. Using shaker incubator (150 rpm) with sawdust as carbon source, laccase yield of (310.45U/l) was obtained at pH 6, temperature 25°C and inoculum size of 105 spores per ml. Thus, sawdust can be categorically stated to be safe, cheap and could be suggested for prospective application of higher production of laccase enzyme in various industries.

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Dual utility of a novel, copper enhanced laccase from Trichoderma aureoviridae

Cited by 19 publications

References 26 publications

Biodegradation of polycyclic aromatic hydrocarbons (PAHs) by fungal enzymes: A review

Biodegradation of polycyclic aromatic hydrocarbons (PAHs) by fungal enzymes: A review

Comparative Study of Dye Decolorization using free and Alginate Gel Entrapped Laccase from Cercospora sp. SPF-6

Screening of Laccase Producing Fungi Using Agro-Wastes under Different Cultural Conditions

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