Dual targets guided screening and isolation of Kukoamine B as a novel natural anti-sepsis agent from traditional Chinese herb Cortex lycii

Liu, Xin; Zheng, Xinchuan; Long, Yupeng; Cao, Hongwei; Wang, Ning; Lu, Yong; Zhao, Kecen; Zhou, Hong; Zheng, Jie

doi:10.1016/j.intimp.2010.10.015

Cited by 26 publications

(24 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…KB is a spermine alkaloids compound firstly isolated from Cortex Lycii. It binds to CpG DNA with high affinity (Kd = 0.66 μM) and inhibits proinflammatory effects induced by CpG DNA in vitro and in vivo [45,46]. Significantly, emodin, rhein and KB are also verified to be antagonists for LPS.…”

Section: Targeting On Tlrs Ligandsmentioning

confidence: 97%

TLRs as pharmacological targets for plant-derived compounds in infectious and inflammatory diseases

Liu¹,

Zheng²,

Zhou³

2011

International Immunopharmacology

Self Cite

View full text Add to dashboard Cite

Section: Targeting On Tlrs Ligandsmentioning

confidence: 97%

TLRs as pharmacological targets for plant-derived compounds in infectious and inflammatory diseases

Liu¹,

Zheng²,

Zhou³

2011

International Immunopharmacology

Self Cite

View full text Add to dashboard Cite

“…Male rats have a longer a longer anogenital than females [28]. Diabetic male rats tend to show increasing blood glucose more early on than female [14]. Also male rats show early symptom of body weight and are more susceptible to early symptoms of diabetes [29].…”

Section: Literature Reviewmentioning

confidence: 99%

“…Research done by Li, Hu, Huang, Han, and Cheung, concluded that Cortex Lycii extract contained a chemical compound that was effective in preventing cell damage[13]. In the few other researches, the chemical compounds of cortex lycii were further broken down[12] [14]. The compound Kukoamine B found in the herb has antibacterial effects.…”

mentioning

confidence: 99%

The Effect of Cortex Lycii on Rattus norvegicus’ Blood Glucose Level Using a High Glucose Diet

Tan¹,

Vinanzaca²

2018

JDM

View full text Add to dashboard Cite

One of the primary problems in the United States is diabetes. Cortex Lycii is an herb from Asia mostly used in traditional Chinese medicine. Although its main purpose is not for lowering blood glucose levels, there are studies on Cortex Lycii with other substances which have proven it can lower glucose level in type 2 diabetes rat. Unlike other experiments, this experiment only used Cortex Lycii as a single unit to see if there was any effect on blood glucose level. Also an observation was done on if Cortex Lycii could reduce blood glucose level after ingesting a high glucose diet. Rattus norvegicus was used in this project and in order for the subject to consume Cortex Lycii, the herb was boiled and the liquid was given for consumption. In order to increase the rats' glucose levels, corn syrup and white sugar were added to the mixture. The data showed significant decrease of blood glucose when Cortex Lycii liquid was ingested. This research has shown that Cortex Lycii can be a potential treatment for hyperglycemia or diabetes. With further research, there can be an alternative treatment rather than injecting external hormones (Insulin).

show abstract

“…This advantage as well as their compatibility are making Chinese medicine especially attractive. While Western medicine employs pure, single compounds, either natural or synthetic, Chinese medicine focuses on combined therapy to relieve disease symptoms, including diabetes (Agrawal et al 2013;Ji et al 2011;Lee 2000;Liu et al 2011). But research is still needed to identify and improve the efficacy of the active principals of herbs both used alone and in combination.…”

Section: Establishment Of Pancreatic B Cell Proliferation Modelmentioning

confidence: 99%

Establishment of a pancreatic β cell proliferation model in vitro and a platform for diabetes drug screening

et al. 2013

View full text Add to dashboard Cite

Diabetes, a disease resulting from loss of functional b cells, is globally an increasingly important condition. Based on the islet-differentiation ability of ductal epithelial cells and stimulating b cell proliferation ability of the Reg Ia gene, we aimed to establish an in vitro pancreatic b cell proliferation model for screening therapeutic drugs of diabetes in the future. Pancreatic ductal epithelial cells were isolated from male Wistar rats, and induced to differentiate into pancreatic b cells. Immunofluorescence staining assay, western blot, RT-PCR analysis, and dithizone staining were used to characterize the cells. Rat Reg Ia protein was transiently expressed in vitro by transfection of HEK 293 cells with the PCMV6-entry-REG Ia plasmid, and expression was verified by RT-PCR analysis, proliferation assay, and apoptosis assay. The pancreatic b cell proliferation model was further validated by a proliferation assay using differentiated pancreatic b cells treated with transfection supernatant. Finally, we have successfully established an in vitro pancreatic b cells proliferation model using transiently expressed rat Reg Ia protein and differentiated pancreatic b cells from pancreatic ductal epithelial cells. This model could be used as a platform to screen new drugs for islet neogenesis to cure diabetes, especially Chinese herbal drugs in the future.

show abstract

Dual targets guided screening and isolation of Kukoamine B as a novel natural anti-sepsis agent from traditional Chinese herb Cortex lycii

Cited by 26 publications

References 28 publications

TLRs as pharmacological targets for plant-derived compounds in infectious and inflammatory diseases

TLRs as pharmacological targets for plant-derived compounds in infectious and inflammatory diseases

The Effect of Cortex Lycii on Rattus norvegicus’ Blood Glucose Level Using a High Glucose Diet

Establishment of a pancreatic β cell proliferation model in vitro and a platform for diabetes drug screening

Contact Info

Product

Resources

About