Dual targeting smart drug delivery system for multimodal synergistic combination cancer therapy with reduced cardiotoxicity

Shubhra, Quazi T.H.; Guo, Kai; Liu, Yixuan; Razzak,; Manir, M.S.; Alam, Ashraful

doi:10.1016/j.actbio.2021.06.016

Cited by 36 publications

(27 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Finally, stabilization by citric acid coating was performed and a stable colloidal solution of MNPs was stored. GOx was attached to the prepared MNPs by EDC/sulfo-NHS coupling reaction following our previously published report . MES buffer (pH 6) was used for the reaction, and the ratio of EDC and sulfo-NHS was 1:1 (molar ratio).…”

Section: Methodsmentioning

confidence: 99%

“…We used a multiple emulsion method to fabricate the DDSs. Previously, we have reported this method and found that microencapsulation of more than two active agents becomes easier, and this method is very efficient, especially when PLGA is used in both inner and outer organic phases …”

Section: Methodsmentioning

confidence: 99%

“…The dose administered was 200 μL (2 mg/kg PTX-equivalent), and the control group received the same volume of saline instead of the drug. For in vivo targeting by a magnetic field, a magnet was placed with surgical tape on the tumor immediately after drug administration (for 1 h) . After the treatment is over (end point: 15 days), mice were sacrificed and serum, tumors, and other organs were collected and stored.…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Engineered Magnetic Polymer Nanoparticles Can Ameliorate Breast Cancer Treatment Inducing Pyroptosis–Starvation along with Chemotherapy

Liu

Guo

Ding

et al. 2022

ACS Appl. Mater. Interfaces

Self Cite

View full text Add to dashboard Cite

Nanotechnology has shown a revolution in cancer treatments, including breast cancers. However, there remain some challenges and translational hurdles. Surgery, radiotherapy, and chemotherapy are the primary treatment methods for breast cancer, although drug combinations showed promising results in preclinical studies. Herein we report the development of a smart drug delivery system (DDS) to efficiently treat breast cancer by pyroptosis–starvation–chemotherapeutic combination. Cancer-starvation agent glucose oxidase was chemically attached to synthesized iron oxide nanoparticles which were entrapped inside poly(lactic-co-glycolic acid) along with apoptosis-associated speck-like protein containing a caspase recruitment domain plasmid and paclitaxel (PTX). An emulsion solvent evaporation method was used to prepare the DDS. The surface of the DDS was modified with chitosan to which aptamer was attached to achieve site-specific targeting. Hence, the prepared DDS could be targeted to a tumor site by both external magnet and aptamer to obtain an enhanced accumulation of drugs at the tumor site. The final size of the aptamer-decorated DDS was less than 200 nm, and the encapsulation efficiency of PTX was 76.5 ± 2.5%. Drug release from the developed DDS was much higher at pH 5.5 than at pH 7.4, ensuring the pH sensitivity of the DDS. Due to efficient dual targeting of the DDS, in vitro viability of 4T1 cells was reduced to 12.1 ± 1.6%, whereas the nontargeted group and free PTX group could reduce the viability of cells to 29.2 ± 2.4 and 46.2 ± 1.6%, respectively. Our DDS showed a synergistic effect in vitro and no severe side effects in vivo. This DDS has strong potential to treat various cancers.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Engineered Magnetic Polymer Nanoparticles Can Ameliorate Breast Cancer Treatment Inducing Pyroptosis–Starvation along with Chemotherapy

Liu

Guo

Ding

et al. 2022

ACS Appl. Mater. Interfaces

Self Cite

View full text Add to dashboard Cite

show abstract

“…Reverse transcription-quantitative (RT-q) PCR. RNA was extracted from the cells following UV exposure and appropriate treatment of asiaticoside and the expression of indicated genes was detected by RT-qPCR following reverse transcription (23). In brief, total RNA was extracted from 1x10 5 HaCat cells by TRIzol ® reagent (Thermo Fisher Scientific, Inc.) and converted to cDNA by PrimeScript RT reagent kit (Takara Bio, Inc.) according to the manufacturer's instructions.…”

Section: Determination Of Superoxide Dismutase (Sod)mentioning

confidence: 99%

Asiaticoside delays senescence and attenuate generation of ROS in UV‑exposure cells through regulates TGF‑β1/Smad pathway

Jiang

Zhou

2022

Exp Ther Med

View full text Add to dashboard Cite

“…Although surgical treatment is not recommended for inflammatory breast cancer and recurrent and metastatic forms [ 2 ]. However, on the other hand, surgery can cause unresected tumors and increase the rates of local relapse [ 3 ]. Therefore, chemotherapy has always been used as the main cancer treatment method.…”

Section: Introductionmentioning

confidence: 99%

Synthesis and Characterization of a Novel Dual-Responsive Nanogel for Anticancer Drug Delivery

Aminoleslami

Porrang

Vahedi

et al. 2022

Oxidative Medicine and Cellular Longevity

View full text Add to dashboard Cite

In this study, to reduce the side effects of anticancer drugs and also to increase the efficiency of current drug delivery systems, a pH and temperature-responsive polymeric nanogel was synthesized by copolymerization of N-vinylcaprolactam (VCL) and acrylic acid (AA) monomers (P(VCL-co-AA)) with a novel cross-linker, triethylene glycol dimethacrylate (TEGDMA), as a biocompatible and nontoxic component. The structural and physicochemical features of the P(VCL-co-AA) nanogel were characterized by FT-IR, DLS/Zeta potential, FE-SEM, and 1HNMR techniques. The results indicated that spherical polymeric nanogel was successfully synthesized with a 182 nm diameter. The results showed that the polymerization process continues with the opening of the carbon-carbon double bond of monomers, which was approved by C-C band removing located at 1600 cm-1. Doxorubicin (Dox) as a chemotherapeutic agent was loaded into the P(VCL-co-AA), whit a significant loading of Dox (83%), and the drug release profile was investigated in the physiological and cancerous site simulated conditions. P(VCL-co-AA) exhibited a pH and temperature-responsive behavior, with an enhanced release rate in the cancerous site condition. The biocompatibility and nontoxicity of P(VCL-co-AA) were approved by MTT assay on the normal human foreskin fibroblasts-2 (HFF-2) cell line. Also, Dox-loaded P(VCL-co-AA) had excellent toxic behavior on the Michigan Cancer Foundation-7 (MCF-7) cell line as model cancerous cells. Moreover, Dox-loaded P(VCL-co-AA) had higher toxicity in comparison with free Dox, which would be a vast advantage in reducing Dox side effects in the clinical cancer treatment applications.

show abstract

Dual targeting smart drug delivery system for multimodal synergistic combination cancer therapy with reduced cardiotoxicity

Cited by 36 publications

References 51 publications

Engineered Magnetic Polymer Nanoparticles Can Ameliorate Breast Cancer Treatment Inducing Pyroptosis–Starvation along with Chemotherapy

Engineered Magnetic Polymer Nanoparticles Can Ameliorate Breast Cancer Treatment Inducing Pyroptosis–Starvation along with Chemotherapy

Asiaticoside delays senescence and attenuate generation of ROS in UV‑exposure cells through regulates TGF‑β1/Smad pathway

Synthesis and Characterization of a Novel Dual-Responsive Nanogel for Anticancer Drug Delivery

Contact Info

Product

Resources

About