Dual-target electrochemical aptasensor based on co-reduced molybdenum disulfide and Au NPs (rMoS2-Au) for multiplex detection of mycotoxins

Han, Zheng; Tang, Zhanmin; Jiang, Ke‐Jian; Huang, Qingwen; Meng, Jiajia; Nie, Dongxia; Zhao, Zhihui

doi:10.1016/j.bios.2019.111894

“…As already noted, assays with a hybridized 96 nt aptamer were mainly correlated to the lowest LOD's through fluorescent [151,152,161,166,170], chemiluminescent [155], optical [169] and MS [197] detections, along with fluorescent and SERS signals obtained from a non-specified hybridized aptamer [177,198]. On the other hand, the shortest assay times were correlated to applications with the 96 nt aptamer in its end-modified [157,160,163,168] and hybridized forms [158,165,173,174], as well as electrochemical designs with some shorter sequences including a thiol modified 80 nt aptamer [156] and an unmodified 40 nt [164] sequence. Likewise, the assay preparation time showed high correlation to 60 nt fluorescent [159], 40 nt electrochemical [167], and 96 nt colorimetric [200] aptasensors.…”

Section: Figurementioning

confidence: 75%

Aptamer-Based Detection of Fumonisin B1: A Systematic Comparison with Conventional and Other Novel Methods

Mirón-Mérida

¹

,

Gong²,

Goycoolea³

2020

Preprint

1

0

View full text Add to dashboard Cite

Mycotoxin contamination is a current issue affecting several crops and processed products worldwide. Among the diverse mycotoxin group, fumonisin B1 (FB1) has become a relevant compound because of its adverse effects in the food chain. Conventional analytical methods previously proposed to quantify FB1 comprise LC-MS, HPLC-FLD and ELISA, while novel approaches integrate different sensing platforms and fluorescently labelled agents in combination with antibodies. Nevertheless, such methods could be expensive, time-consuming and require experience. Aptamers (ssDNA) are promising alternatives to overcome some of the drawbacks of conventional analytical methods, their high affinity through specific aptamer-target binding has been exploited in various designs attaining favorable limits of detection (LOD). So far, two aptamers specific to FB1 have been reported, and their modified and shortened sequences have been explored for a successful target quantification. In this critical review spanning the last eight years, we have conducted a systematic comparison based on principal component analysis of the aptamer-based techniques for FB1, compared with chromatographic, immunological and other analytical methods. We have also conducted an <i>in-silico</i> prediction of the folded structure of both aptamers under their reported conditions. The potential of aptasensors for the future development of highly sensitive FB1 testing methods is emphasized.

show abstract

“…The ferrocenyl-containing nanobioconjugates linked then to the target molecules, thereby producing signals by DPV that were correlated with changes in their concentrations. The dual electrochemical biosensor responded in a linear range from 1 µg/mL to 10 ng/mL and 1 µg/mL to 100 ng/mL for ZEN and FB1, respectively, with a LOD of 0.5 µg/mL in both cases [ 131 ].…”

Section: Nanobioconjugates In Biosensingmentioning

confidence: 99%

Nanobioconjugates for Signal Amplification in Electrochemical Biosensing

Cajigas

¹

,

Orozco

²

2020

View full text Add to dashboard Cite

Nanobioconjugates are hybrid materials that result from the coalescence of biomolecules and nanomaterials. They have emerged as a strategy to amplify the signal response in the biosensor field with the potential to enhance the sensitivity and detection limits of analytical assays. This critical review collects a myriad of strategies for the development of nanobioconjugates based on the conjugation of proteins, antibodies, carbohydrates, and DNA/RNA with noble metals, quantum dots, carbon- and magnetic-based nanomaterials, polymers, and complexes. It first discusses nanobioconjugates assembly and characterization to focus on the strategies to amplify a biorecognition event in biosensing, including molecular-, enzymatic-, and electroactive complex-based approaches. It provides some examples, current challenges, and future perspectives of nanobioconjugates for the amplification of signals in electrochemical biosensing.

show abstract

“…From all the biosensing designs applying the 96 nt aptamer, the most sensitive were those transduced into fluorescent [154,166,170] and electrochemical [172,173,174] signals. The first fluorescent method described the application of avidin-modified fluorescent nanoparticles and graphene oxide (GO), as donor/acceptor pair in Förster resonance energy transfer (FRET).…”

Section: Fluorescent Detectionmentioning

confidence: 99%

“…This approach included a DNA sequence complementary on different segments to two aptamers and the addition of gold nanorods for signal enlargement related to concentrations of FB1 as low as 0.00026 µg/L [174]. Another technique in the pg/L scale (0.0005 µg/L) was designed on a glassy carbon electrode modified with molybdenum disulfide and gold nanoparticles for the attachment of aptamers and further immobilization with labelled cDNA, whose differential pulse voltammetry (DPV) decreased with the addition of FB1 [173].…”

Section: Electrochemical Detectionmentioning

confidence: 99%

Aptamer-Based Detection of Fumonisin B1: A Systematic Comparison with Conventional and Other Novel Methods

Mirón-Mérida¹,

Gong²,

Goycoolea³

2020

Preprint

0

View full text Add to dashboard Cite

Mycotoxin contamination is a current issue affecting several crops and processed products worldwide. Among the diverse mycotoxin group, fumonisin B1 (FB1) has become a relevant compound because of its adverse effects in the food chain. Conventional analytical methods previously proposed to quantify FB1 comprise LC-MS, HPLC-FLD and ELISA, while novel approaches integrate different sensing platforms and fluorescently labelled agents in combination with antibodies. Nevertheless, such methods could be expensive, time-consuming and require experience. Aptamers (ssDNA) are promising alternatives to overcome some of the drawbacks of conventional analytical methods, their high affinity through specific aptamer-target binding has been exploited in various designs attaining favorable limits of detection (LOD). So far, two aptamers specific to FB1 have been reported, and their modified and shortened sequences have been explored for a successful target quantification. In this critical review spanning the last eight years, we have conducted a systematic comparison based on principal component analysis of the aptamer-based techniques for FB1, compared with chromatographic, immunological and other analytical methods. We have also conducted an <i>in-silico</i> prediction of the folded structure of both aptamers under their reported conditions. The potential of aptasensors for the future development of highly sensitive FB1 testing methods is emphasized.

show abstract

Dual-target electrochemical aptasensor based on co-reduced molybdenum disulfide and Au NPs (rMoS2-Au) for multiplex detection of mycotoxins

Cited by 82 publications

References 33 publications

Aptamer-Based Detection of Fumonisin B1: A Systematic Comparison with Conventional and Other Novel Methods

Aptamer-Based Detection of Fumonisin B1: A Systematic Comparison with Conventional and Other Novel Methods

Nanobioconjugates for Signal Amplification in Electrochemical Biosensing

Aptamer-Based Detection of Fumonisin B1: A Systematic Comparison with Conventional and Other Novel Methods

Contact Info

Product

Resources

About