Dual-signal aptamer sensor based on polydopamine-gold nanoparticles and exonuclease I for ultrasensitive malathion detection

Xu, Guoli; Hou, Jingzhou; Zhao, Yuechao; Bao, Jing; Yang, Mei; Fa, Huanbao; Yang, Yixia; Li, Li; Huo, Danqun; Hou, Changjun

doi:10.1016/j.snb.2019.01.113

Cited by 89 publications

(25 citation statements)

References 53 publications

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“…The electrochemical method was integrated with amplification strategies for assaying numerous targets, such as sulfadimethoxine, malathion, OTA, kanamycin, ampicillin, atrazine (ATZ), and acetamiprid [ 138 , 203 , 204 , 205 , 206 , 207 , 208 , 209 , 210 ]. Sulfadimethoxine was assessed electrochemically with the assistance of nuclease amplification ( Figure 11 A), in which a gold surface of an electrode was immobilized with a DNA probe, and then hybridized with a specific aptamer for sulfadimethoxine, forming a dsDNA that inhibited their digestion via nuclease P1.…”

Section: Electrochemical Aptasensormentioning

confidence: 99%

“…Moreover, the electrodeposition synthesis of PDA–AuNPs provided satisfactory biocompatibility and electrical conductivity for the sensor. Exo I was used to promote the autocatalytic cycling of malathion by enhancing the current change, with a linear response range of 0.5–600 ng/L [ 204 ]. OTA and ATZ were detected electrochemically and photoelectrochemically with the assistance of cycling amplification, with a linear range of 0.01 to 1 ng/mL and a LOD of 0.004 ng/mL for OTA [ 205 ] and linear range of 50 fM to 0.3 nM with LOD of 12.0 fM for ATZ [ 206 ], as outlined in Figure 11 C,D.…”

Section: Electrochemical Aptasensormentioning

confidence: 99%

“…( A ) Assaying of sulfadimethoxine using gold electrode fabricated with dsDNA consisting of DNA probe and specific aptamer for sulfadimethoxine inhibiting nuclease digestion, reproduced from [ 203 ]. ( B ) Malathion assay with the assistance of Exo I as a signal enhancer and layer of PDA-AuNPs deposited on GCE as an electrical conductivity enhancer, reproduced from [ 204 ]. ( C ) OTA assay using TiO 2 nanotube electrode functionalized with DNA probe tagged with MB, reproduced from [ 205 ].…”

Section: Figurementioning

confidence: 99%

“…Aptamers have intrinsic advantages, such as their availability for both chemical modifications and conjugation with different labels, facilitating their ability to be used to construct a sensitive and highly selective platform sensor. In this review, recent advances in the [204]. (C) OTA assay using TiO 2 nanotube electrode functionalized with DNA probe tagged with MB, reproduced from [205].…”

Section: Conclusion and Future Outlookmentioning

confidence: 99%

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Recent Advances in Aptamer Sensors

Shaban

Kim

2021

Sensors

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Recently, aptamers have attracted attention in the biosensing field as signal recognition elements because of their high binding affinity toward specific targets such as proteins, cells, small molecules, and even metal ions, antibodies for which are difficult to obtain. Aptamers are single oligonucleotides generated by in vitro selection mechanisms via the systematic evolution of ligand exponential enrichment (SELEX) process. In addition to their high binding affinity, aptamers can be easily functionalized and engineered, providing several signaling modes such as colorimetric, fluorometric, and electrochemical, in what are known as aptasensors. In this review, recent advances in aptasensors as powerful biosensor probes that could be used in different fields, including environmental monitoring, clinical diagnosis, and drug monitoring, are described. Advances in aptamer-based colorimetric, fluorometric, and electrochemical aptasensing with their advantages and disadvantages are summarized and critically discussed. Additionally, future prospects are pointed out to facilitate the development of aptasensor technology for different targets.

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Section: Electrochemical Aptasensormentioning

confidence: 99%

Section: Electrochemical Aptasensormentioning

confidence: 99%

Section: Figurementioning

confidence: 99%

Section: Conclusion and Future Outlookmentioning

confidence: 99%

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Recent Advances in Aptamer Sensors

Shaban

Kim

2021

Sensors

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“…AuNPs arrived at the pharmaceutical and medical industry due to their intrinsic properties because they can be synthesized in different sizes and morphologies. In addition, the reduction from Au +3 to Au 0 facilitates their functionalization with ligands, such as aptamers, polymers, drugs, and genetic material, among others [ 215 , 216 , 217 ]. In addition, the chemical inertness of gold allows good in vitro and in vivo biocompatibility [ 218 ], so there has been a proposal to functionalize AuNPs surfaces with antibiotics to increase antibacterial efficacy against resistant pathogens [ 219 ].…”

Section: Nanotechnology Applied To Antimicrobial Resistancementioning

confidence: 99%

Antimicrobial Resistance and Inorganic Nanoparticles

Balderrama-González

Piñón-Castillo

Ramírez-Valdespino

et al. 2021

IJMS

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Antibiotics are being less effective, which leads to high mortality in patients with infections and a high cost for the recovery of health, and the projections that are had for the future are not very encouraging which has led to consider antimicrobial resistance as a global health problem and to be the object of study by researchers. Although resistance to antibiotics occurs naturally, its appearance and spread have been increasing rapidly due to the inappropriate use of antibiotics in recent decades. A bacterium becomes resistant due to the transfer of genes encoding antibiotic resistance. Bacteria constantly mutate; therefore, their defense mechanisms mutate, as well. Nanotechnology plays a key role in antimicrobial resistance due to materials modified at the nanometer scale, allowing large numbers of molecules to assemble to have a dynamic interface. These nanomaterials act as carriers, and their design is mainly focused on introducing the temporal and spatial release of the payload of antibiotics. In addition, they generate new antimicrobial modalities for the bacteria, which are not capable of protecting themselves. So, nanoparticles are an adjunct mechanism to improve drug potency by reducing overall antibiotic exposure. These nanostructures can overcome cell barriers and deliver antibiotics to the cytoplasm to inhibit bacteria. This work aims to give a general vision between the antibiotics, the nanoparticles used as carriers, bacteria resistance, and the possible mechanisms that occur between them.

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