Dual signal amplification strategy for amperometric aptasensing using hydroxyapatite nanoparticles. Application to the sensitive detection of the cancer biomarker platelet-derived growth factor BB

Jiang, Wentao; Tian, Dazhi; Zhang, Li; Guo, Qingjun; Cui, Yujun; Yang, Minghui

doi:10.1007/s00604-017-2471-1

Cited by 35 publications

(5 citation statements)

References 43 publications

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“…On the other hand, the electrode with modified AuNPs shows two pairs of robust current peaks (Figure , curve c). This strong electrochemical current is due to the reaction of the dC 20 DNA on the AuNP surface, specifically the reaction of the phosphate backbone of dC 20 with molybdate to form the redox-active molybdophosphate precipitate as shown in our previous reports. − This previous work probed in detail the reaction of DNA strands composed of different nucleotide bases with molybdate, leading to the possibility that the polycytosine sequence generates the highest current intensity . As in the description above, electron transfer within the molybdophosphate leads to two pairs of redox peaks (0.22 and 0.35 V).…”

Section: Resultsmentioning

confidence: 65%

Polycytosine DNA Electric-Current-Generated Immunosensor for Electrochemical Detection of Human Epidermal Growth Factor Receptor 2 (HER2)

Shen

Yang

et al. 2018

Anal. Chem.

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A polycytosine DNA-based immunosensor for electrochemical detection was developed and tested for detection of human epidermal growth factor receptor 2 (HER2), a breast cancer biomarker. We utilized gold nanoparticles (AuNPs) as supporting matrix to immobilize polycytosine DNA sequence (dC) for electrochemical current generation and anti-HER2 antibodies. In the presence of target HER2, a sandwiched immunocomplex forms between a peptide specific to HER2 immobilized on the gold electrode and the anti-HER2 antibodies on the AuNPs. The HER2 captured by the sensor is detected because of the reaction of the dC phosphate backbone with molybdate, forming redox molybdophosphate precipitate that generates an electrochemical current on the surface of the electrode. The assay is sensitive: the calculated limit of detection of HER2 was as low as 0.5 pg/mL and the detection was linear to HER2 from 1 pg/mL to 1 ng/mL. The sensor's specificity is high, and there is no cross reactivity with several potential interferents, such as human IgG, human IgA, p53, carcinoembryonic antigen, and protein kinase. The sensor's performance with HER2 in clinical serum samples is similar to the performance of commercial ELISA assays. The configuration of polycytosine DNA as electrochemical current generating label and anti-HER2 antibodies on AuNPs is versatile and can be reconfigured to detect low levels of different analytes, or made more sensitive by amplifying the DNA to produce more phosphate to react with NaMoO

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Section: Resultsmentioning

confidence: 65%

Polycytosine DNA Electric-Current-Generated Immunosensor for Electrochemical Detection of Human Epidermal Growth Factor Receptor 2 (HER2)

Shen

Yang

et al. 2018

Anal. Chem.

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“…In actual sample, 100–1000 times the interfering substances are seen, but most of the interference experiments have reported so far the target and interference at a concentration ratio of 1 : 10. 50–52 Therefore, we have selected GPC3 with a concentration of 10.0 μg mL −1 and interfering protein with a concentration of 100.0 μg mL −1 for measurement and compared the DPV peak current signals, and the results are shown in Fig. 6A.…”

Section: Resultsmentioning

confidence: 99%

A label-free electrochemical aptasensor based on platinum@palladium nanoparticles decorated with hemin-reduced graphene oxide as a signal amplifier for glypican-3 determination

et al. 2022

Biomater. Sci.

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“…As also reported in our recent review (Arduini et al, 2017), many electrochemical (bio)sensors have been developed for biomedical applications (Huang X. et al, 2017;Jiang et al, 2017). This manuscript was inspired by the unequal use of sensors in the pharmaceutical sector, where optical devices are abundant while the electrochemical ones are still in their infancy, despite their potential has been already demonstrated.…”

Section: Introductionmentioning

confidence: 88%

Paper-Based Electrochemical Devices for the Pharmaceutical Field: State of the Art and Perspectives

Antonacci

Scognamiglio

Mazzaracchio

et al. 2020

Front. Bioeng. Biotechnol.

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The current international pharmaceutical scenario encompasses several steps in drug production, with complex and extremely long procedures. In the last few decades, scientific research has been trying to offer valid and reliable solutions to replace or support conventional techniques, in order to facilitate drug development procedures. These innovative approaches may have extremely positive effects in the production chain, supplying fast, and cost-effective quality as well as safety tests on active pharmaceutical ingredients (APIs) and their excipients. In this context, the exploitation of electrochemical paper-based analytical devices (ePADs) is still in its infancy, but is particularly promising in the detection of APIs and excipients in tablets, capsules, suppositories, and injections, as well as for pharmacokinetic bioanalysis in real samples.

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Dual signal amplification strategy for amperometric aptasensing using hydroxyapatite nanoparticles. Application to the sensitive detection of the cancer biomarker platelet-derived growth factor BB

Cited by 35 publications

References 43 publications

Polycytosine DNA Electric-Current-Generated Immunosensor for Electrochemical Detection of Human Epidermal Growth Factor Receptor 2 (HER2)

Polycytosine DNA Electric-Current-Generated Immunosensor for Electrochemical Detection of Human Epidermal Growth Factor Receptor 2 (HER2)

A label-free electrochemical aptasensor based on platinum@palladium nanoparticles decorated with hemin-reduced graphene oxide as a signal amplifier for glypican-3 determination

Paper-Based Electrochemical Devices for the Pharmaceutical Field: State of the Art and Perspectives

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