Dual-SERS biosensor for one-step detection of microRNAs in exosome and residual plasma of blood samples for diagnosing pancreatic cancer

Pang, Yuanfeng; Wang, Chaoguang; Lu, Luchun; Wang, Chongwen; Sun, Zhiwei; Xiao, Rui

doi:10.1016/j.bios.2019.01.039

Cited by 199 publications

(114 citation statements)

References 45 publications

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“…The limit of detection (LOD) of the SiO 2 @PEI-QD-based strip was estimated to be 5 Â 10 2 cells per mL, with the LOD dened as the 3 : 1 threshold ratio with respect to the blank signal. [24][25][26] These results revealed that the proposed LFA strip with SiO 2 @PEI-QDs as the uorescence label displayed high sensitivity and a wide dynamic range for S. typhi detection. For comparison, the S. typhi concentrations in test milk samples were also conrmed using the traditional plate counting method.…”

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confidence: 77%

Polyethyleneimine-interlayered silica-core quantum dot-shell nanocomposites for sensitive detection of Salmonella typhimurium via a lateral flow immunoassay

et al. 2020

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confidence: 77%

Polyethyleneimine-interlayered silica-core quantum dot-shell nanocomposites for sensitive detection of Salmonella typhimurium via a lateral flow immunoassay

et al. 2020

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“…This process is repeated thousands of times, as the miRNA is not cleaved by the DSN, allowing the amplification of the signal for identifying that specific miRNA to a detection limit of 1 aM [159]. The group furtherly investigated the expression level of miRNA-10b from plasma-derived exosomes and supernatant plasma obtained from pancreatic ductal adenocarcinoma and chronic prostatitis patients and validated that the method could accurately differentiate between the two groups and the healthy control group [159]. A variant of this method was previously employed to characterize exosomal miRNA extracted from non-small-cell lung adenocarcinoma patients' plasma.…”

Section: Detection Of Ev/exosomal Mirna Using Sers Based Methodsmentioning

confidence: 99%

MicroRNAs from Liquid Biopsy Derived Extracellular Vesicles: Recent Advances in Detection and Characterization Methods

Drula

Ott

Berindan‐Neagoe

et al. 2020

Cancers

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Liquid biopsies have become a convenient tool in cancer diagnostics, real-time disease monitoring, and evaluation of residual disease. Yet, the information still encrypted in the variety of tumor-derived molecules identified in biofluids has proven difficult to decipher due to the technological limitations imposed by their biological nature. Such is the case of extracellular vesicle (EV) encapsulated ncRNAs, which have gained traction in recent years as biomarkers. Due to their resilience towards degrading factors they may act as suitable disease indicators. This review addresses the less described issues in this context. We present an overview of less investigated biofluids that can be used for EV isolation in addition to different isolation approaches to overcome the technical challenges these specimens harbor. Furthermore, we summarize the latest technological advances providing improvement to ncRNA detection and analysis. Thereby, this review summarizes the current state-of-the-art methodologies regarding EV and EV derived miRNA analysis and how they compare to current approaches.

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“…There are some advantages coming with the discovery of these exosomes, as they are found in a large amount in body fluids, they are more stable in circulation, easy to separate, handle and describe (65). As science is gaining a lot of information in the domain of tumor genesis and diagnosis using exosomes, research focused on the detection of exosomes using SERS approaches were described for the detection of CTCs and other tumor biomarkers (66)(67)(68).…”

Section: Tumor Biomarkers From Body Fluids Targeted With Sersmentioning

confidence: 99%

Recent advances in surface‑enhanced Raman spectroscopy based liquid biopsy for colorectal cancer (Review)

et al. 2020

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As colorectal cancer (CRC) is one of the forms of cancer with the highest prevalence globally and with a high mortality, screening and early detection remains a major issue. Colonoscopy is still the gold standard for detecting premalignant lesions, but it is burdened by some complications. For instance, it is laborious, with some difficulties of acceptance for some patients, and is ultimately an imperfect standard, given that some premalignant lesions or incipient malignancies can be missed by colonoscopic evaluation. In this context, new non-invasive approaches such as surface-enhanced Raman spectroscopy (SERS) based liquid biopsy have gained ground in recent years, showing promising results in oncological pathology diagnosis. These new methods have enabled the detection of subtle molecular profile alterations prior to any macroscopic morphological changes, thus providing a useful tool for early CRC detection. In the present review, we provide a summary of published studies applying SERS in CRC detection, along with our personal experience in using SERS in the diagnosis of different oncological pathologies, including CRC.

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Dual-SERS biosensor for one-step detection of microRNAs in exosome and residual plasma of blood samples for diagnosing pancreatic cancer

Cited by 199 publications

References 45 publications

Polyethyleneimine-interlayered silica-core quantum dot-shell nanocomposites for sensitive detection of Salmonella typhimurium via a lateral flow immunoassay

Polyethyleneimine-interlayered silica-core quantum dot-shell nanocomposites for sensitive detection of Salmonella typhimurium via a lateral flow immunoassay

MicroRNAs from Liquid Biopsy Derived Extracellular Vesicles: Recent Advances in Detection and Characterization Methods

Recent advances in surface‑enhanced Raman spectroscopy based liquid biopsy for colorectal cancer (Review)

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