Dual selective sensor for exosomes in serum using magnetic imprinted polymer isolation sandwiched with aptamer/graphene oxide based FRET fluorescent ignition

Feng, Dongwei; Ren, Maozhi; Miao, Yunfei; Liao, Zerong; Zhang, Tuanjie; Chen, Shi; Ye, Kaida; Zhang, Pengjie; Ma, Xiaolan; Ni, Jiati; Li, Huanjun; Peng, Jirun; Luo, Aiqin; Li, Geng; Deng, Yulin

doi:10.1016/j.bios.2022.114112

Cited by 40 publications

(19 citation statements)

References 90 publications

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“…The 3D porous serpentine nanostructures were innovatively constructed, and multiplexed detection of exosomes was allowed by this chip. In addition, it has shown that the dual-selective fluorescent nanosensor (Feng et al, 2022), 3D nanomachine based on Janus wireframe DNA cube (Xu et al, 2022), and 3D molecular beacon based on cubic DNA nanospaces (Mao et al, 2022) can be used for the characterization and analysis of exosomes (Guo et al, 2021b).…”

Section: Capture By 3d Zno Nanoarraysmentioning

confidence: 99%

Recent developments in isolating methods for exosomes

Gao

et al. 2023

Front. Bioeng. Biotechnol.

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Exosomes are the smallest extracellular vesicles that can be released by practically all cell types, and range in size from 30 nm to 150 nm. As the major marker of liquid biopsies, exosomes have great potential for disease diagnosis, therapy, and prognosis. However, their inherent heterogeneity, the complexity of biological fluids, and the presence of nanoscale contaminants make the isolation of exosomes a great challenge. Traditional isolation methods of exosomes are cumbersome and challenging with complex and time-consuming operations. In recent years, the emergence of microfluidic chips, nanolithography, electro-deposition, and other technologies has promoted the combination and innovation of the isolation methods. The application of these methods has brought very considerable benefits to the isolation of exosomes such as ultra-fast, portable integration, and low loss. There are significant functional improvements in isolation yield, isolation purity, and clinical applications. In this review, a series of methods for the isolation of exosomes are summarized, with emphasis on the emerging methods, and in-depth comparison and analysis of each method are provided, including their principles, merits, and demerits.

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Section: Capture By 3d Zno Nanoarraysmentioning

confidence: 99%

Recent developments in isolating methods for exosomes

Gao

et al. 2023

Front. Bioeng. Biotechnol.

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“…These properties have been recently used for molecular detection and analysis of circulating biomarkers including EVs [ 185 ]. The electrical property of graphene and graphene oxide provide an excellent opportunity to design field-effect-transistor (FET)-based biosensors for EV detection [ 186 , 187 , 188 , 189 , 190 ]. For example, Tsang et al fabricated a back-gated graphene FET biosensor by depositing a graphene monolayer onto a microfluidic device by chemical vapor deposition [ 186 ].…”

Section: Carbon Nanomaterialsmentioning

confidence: 99%

Nanomaterials for Molecular Detection and Analysis of Extracellular Vesicles

et al. 2023

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Extracellular vesicles (EVs) have emerged as a novel resource of biomarkers for cancer and certain other diseases. Probing EVs in body fluids has become of major interest in the past decade in the development of a new-generation liquid biopsy for cancer diagnosis and monitoring. However, sensitive and specific molecular detection and analysis are challenging, due to the small size of EVs, low amount of antigens on individual EVs, and the complex biofluid matrix. Nanomaterials have been widely used in the technological development of protein and nucleic acid-based EV detection and analysis, owing to the unique structure and functional properties of materials at the nanometer scale. In this review, we summarize various nanomaterial-based analytical technologies for molecular EV detection and analysis. We discuss these technologies based on the major types of nanomaterials, including plasmonic, fluorescent, magnetic, organic, carbon-based, and certain other nanostructures. For each type of nanomaterial, functional properties are briefly described, followed by the applications of the nanomaterials for EV biomarker detection, profiling, and analysis in terms of detection mechanisms.

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“…The application of MIPs to pre-existing sensing mechanisms can significantly improve their sensitivity. Feng et al developed a fluorescence resonance energy transfer system (FRET) for the detection of exosomes in the serum of breast cancer patients with an LOD of 2.43 × 10 6 particles/mL [ 95 ]. The sensor consisted of two elements that formed a sandwich in the presence of the target of interest: an MIP capture element and an aptamer/graphene oxide (GO) selective ‘turn-on’ component.…”

Section: Mip-based Cancer Diagnosismentioning

confidence: 99%

Molecularly imprinted polymers (MIPs): emerging biomaterials for cancer theragnostic applications

et al. 2023

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Cancer is a disease caused by abnormal cell growth that spreads through other parts of the body and threatens life by destroying healthy tissues. Therefore, numerous techniques have been employed not only to diagnose and monitor the progress of cancer in a precise manner but also to develop appropriate therapeutic agents with enhanced efficacy and safety profiles. In this regard, molecularly imprinted polymers (MIPs), synthetic receptors that recognize targeted molecules with high affinity and selectivity, have been intensively investigated as one of the most attractive biomaterials for theragnostic approaches. This review describes diverse synthesis strategies to provide the rationale behind these synthetic antibodies and provides a selective overview of the recent progress in the in vitro and in vivo targeting of cancer biomarkers for diagnosis and therapeutic applications. Taken together, the topics discussed in this review provide concise guidelines for the development of novel MIP-based systems to diagnose cancer more precisely and promote successful treatment. Graphical Abstract Molecularly imprinted polymers (MIPs), synthetic receptors that recognize targeted molecules with high affinity and selectivity, have been intensively investigated as one of the most attractive biomaterials for cancer theragnostic approaches. This review describes diverse synthesis strategies to provide the rationale behind these synthetic antibodies and provides a selective overview of the recent progress in the in vitro and in vivo targeting of cancer biomarkers for diagnosis and therapeutic applications. The topics discussed in this review aim to provide concise guidelines for the development of novel MIP-based systems to diagnose cancer more precisely and promote successful treatment.

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Dual selective sensor for exosomes in serum using magnetic imprinted polymer isolation sandwiched with aptamer/graphene oxide based FRET fluorescent ignition

Cited by 40 publications

References 90 publications

Recent developments in isolating methods for exosomes

Recent developments in isolating methods for exosomes

Nanomaterials for Molecular Detection and Analysis of Extracellular Vesicles

Molecularly imprinted polymers (MIPs): emerging biomaterials for cancer theragnostic applications

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