Dual Roles for PARP1 during Heat Shock: Transcriptional Activator and Posttranscriptional Inhibitor of Gene Expression

Vyas, Sejal; Chang, Paul

doi:10.1016/j.molcel.2012.12.017

Cited by 18 publications

(27 citation statements)

References 9 publications

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“…In Bmal1 fl/fl ; CCSP -icre +/− mice, GR recruitment to Cxcl5 was reduced and no longer rhythmic (Fig 5e). Acetylation of lysine 27 on histone H3 (H3/K27Ac) is a robust epigenetic mark of active enhancers 32,33 . We measured H3/K27Ac to determine enhancer activity and gauge the consequence of GR recruitment.…”

Section: Resultsmentioning

confidence: 99%

An epithelial circadian clock controls pulmonary inflammation and glucocorticoid action

Gibbs

Ince

Matthews

et al. 2014

Nat Med

365

424

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The circadian system is as an important regulator of immune function. Human inflammatory lung diseases frequently show time-of-day variation in symptom severity and lung function, but the mechanisms and cell types that are underlying these effects remain unclear. We show that pulmonary antibacterial responses are modulated by a circadian clock within epithelial club (Clara) cells. These drive circadian neutrophil recruitment to the lung via the chemokine CXCL5. Genetic ablation of the clock gene Bmal1 (also called Arntl or MOP3) in bronchiolar cells disrupts rhythmic Cxcl5 expression, resulting in exaggerated inflammatory responses to lipopolysaccharide and bacterial infection. Adrenalectomy blocks rhythmic inflammatory responses and the circadian regulation of CXCL5, suggesting a key role for the adrenal axis in driving CXCL5 expression and pulmonary neutrophil recruitment. Glucocorticoid receptor occupancy at the Cxcl5 locus shows circadian oscillations, but this is disrupted in mice with bronchiole-specific ablation of Bmal1, leading to enhanced CXCL5 expression despite normal corticosteroid secretion. In clock-gene disrupted mice the synthetic glucocorticoid dexamethasone loses anti-inflammatory efficacy. We now define a regulatory mechanism that links the circadian clock and glucocorticoid hormones to control both time-of-day variation and also the magnitude of pulmonary inflammation and responses to bacterial infection.

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Section: Resultsmentioning

confidence: 99%

An epithelial circadian clock controls pulmonary inflammation and glucocorticoid action

Gibbs

Ince

Matthews

et al. 2014

Nat Med

365

424

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“…B-2 B cell development is dependent on an intact HSC compartment (Hardy, 2007), and low PU.1 expression can result in loss of HSCs (Iwasaki et al, 2005; Staber et al, 2013). However, HSCs were present in fetal and adult UREΔ/Δ mice (Figure S5) and exhibited myeloid and lymphoid potential when transplanted into lethally irradiated recipients (data not shown).…”

Section: Resultsmentioning

confidence: 99%

“…A requirement for precise regulation of PU.1 expression during hematopoietic development has been extensively discussed (Dakic et al, 2007; Rosenbauer et al, 2006; Staber et al, 2013). This is achieved through the binding of specific combinations of transcription factors that include Runx1, Sfpi1, Ikzf and/or Foxo1 to multiple Sfpi1 cis-regulatory elements (Leddin et al, 2011; Okuno et al, 2005; Zarnegar et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Distinct Genetic Networks Orchestrate the Emergence of Specific Waves of Fetal and Adult B-1 and B-2 Development

et al. 2016

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SUMMARY B cell development is often depicted as a linear process initiating in the fetus and continuing postnatally. Using a PU.1 hypomorphic mouse model, we found that B-1 and B-2 lymphopoiesis occurred in distinct fetal and adult waves differentially dependent on the Sfpi1 14 kB upstream regulatory element. The initial wave of fetal B-1 development was absent in PU.1 hypomorphic mice, while subsequent fetal and adult waves emerged. In contrast, B-2 lymphopoiesis occurred in distinct fetal and adult waves. Whole transcriptome profiling of fetal and adult B cell progenitors supported the existence of three waves of B-1 and two waves of B-2 development, and revealed that the network of transcription factors governing B lineage specification and commitment was highly divergent between B-1 and B-2 progenitors. These findings support the view that the B-1 and B-2 lineages are distinct and provide a genetic basis for layering of immune system development.

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“…Our recent work implicated Pol V-produced lncRNA in nucleosome positioning by indirectly recruiting a SWI/SNF chromatin remodeling complex via the IDN2 protein [15]. Being involved in the establishment of DNA methylation and repressive histone modifications as well as changes in nucleosome positioning, Pol V-produced lncRNA has broad effects on chromatin status and has been proposed to control genome activity [3].…”

Section: Introductionmentioning

confidence: 99%

“…An additional approach is identifying binding sites of proteins recruited by Pol V transcripts [5]. Further investigation of the functional significance of those lncRNAs requires the ability to directly detect their presence and study their interactions with proteins [7],[9],[15],[16],[17]. We present methods, which allow the study of Pol V produced lncRNA as well as transcripts produced by other RNA polymerases.…”

Section: Introductionmentioning

confidence: 99%

Analysis of long non-coding RNAs produced by a specialized RNA polymerase in Arabidopsis thaliana

Rowley

Böhmdorfer

Wierzbicki

2013

Methods

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Long non-coding RNAs (lncRNAs) play important roles in several processes including control of gene expression. In Arabidopsis thaliana, a class of lncRNAs is produced by a specialized RNA Polymerase V (Pol V), which is involved in controlling genome activity by transcriptional gene silencing. lncRNAs produced by Pol V have been proposed to serve as scaffolds for binding of several silencing factors which further mediate the establishment of repressive chromatin modifications. We present methods for discovery and characterization of lncRNAs produced by Pol V. Chromatin Immunoprecipitation coupled with deep sequencing (ChIP-seq) allows discovery of genomic regions bound by proteins in a manner dependent on either Pol V or transcripts produced by Pol V. RNA Immunoprecipitation (RIP) allows testing lncRNA-protein interactions at identified loci. Finally, real-time RT-PCR allows detection of low abundance Pol V transcripts from total RNA. These methods may be more broadly applied to discovery and characterization of RNAs produced by distinct RNA Polymerases.

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Dual Roles for PARP1 during Heat Shock: Transcriptional Activator and Posttranscriptional Inhibitor of Gene Expression

Abstract: In this issue of Molecular Cell, Di Giammartino et al. (2012) identify a new function for PARP1 in the post-transcriptional regulation of mRNAs via ADP-ribosylation of poly(A) polymerase, a mRNA 3′ processing enzyme.

Cited by 18 publications

References 9 publications

An epithelial circadian clock controls pulmonary inflammation and glucocorticoid action

An epithelial circadian clock controls pulmonary inflammation and glucocorticoid action

Distinct Genetic Networks Orchestrate the Emergence of Specific Waves of Fetal and Adult B-1 and B-2 Development

Analysis of long non-coding RNAs produced by a specialized RNA polymerase in Arabidopsis thaliana

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