Dual Role of Toll-Like Receptors in Asthma and Chronic Obstructive Pulmonary Disease

Bezemer, Gillina; Sagar, Seil; Bergenhenegouwen, Jeroen van; Georgiou, Niki; Garssen, Johan; Kraneveld, Aletta D.; Folkerts, Gert

doi:10.1124/pr.111.004622

Cited by 100 publications

(80 citation statements)

References 291 publications

(303 reference statements)

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“…Thus, Myd88 2/2 mice show ameliorated allergic responses to these allergens when exposed to lungs and develop decreased Ag-specific IgE production together with defective Th2 cytokine production and inflammatory cell lung infiltration (24,25). Therefore, MyD88 signaling, which initiates or exacerbates allergic asthma, might be mediated by TLRs on DCs or other innate cell populations (23). However, the role of B cell-intrinsic MyD88 signaling in the production of allergen-specific IgE in lung allergic responses has not been determined.…”

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“…However, it is now recognized that MyD88 signaling has an important role in allergic disease development (22,23), and several allergens were reported to activate MyD88 signaling. Lung sensitization of Ags with low-dose LPS is a well-characterized model for inducing airway allergic symptoms (23). Natural airborne allergens, such as house dust mite (24) or the fungus Alternaria alternata (25), contain TLR ligands that mediate their allergenic activity.…”

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B Cell–Intrinsic MyD88 Signaling Is Essential for IgE Responses in Lungs Exposed to Pollen Allergens

Matsushita

Yoshimoto

2014

The Journal of Immunology

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Allergen-specific IgE is linked to asthma pathogenesis, but the underlying mechanisms of IgE production in response to allergen exposure are poorly understood. In this article, we show that B cell–intrinsic MyD88 is essential for IgE/IgG1 production evoked by ragweed pollen instilled into lungs. MyD88-deficient mice showed defective IgE/IgG1 production and germinal center responses to lung instillation of ragweed pollen. However, MyD88 was dispensable for dendritic cell activation and Th2 cell development. B cell–specific deletion of MyD88 replicated the defective Ab production observed in MyD88-deficient mice. Although ragweed pollen contains TLR ligands, TLR2/4/9-deficient mice developed normal allergic responses to ragweed pollen. However, anti–IL-1R1 Ab-treated mice and IL-18–deficient mice showed decreased IgE/IgG1 production with normal Th2 development. Furthermore, B cell–specific MyD88-deficient mice showed reduced IgE/IgG1 production in response to lung instillation of OVA together with IL-1α, IL-1β, or IL-18. Thus, pollen instillation into lungs induces IL-1α/β and IL-18 production, which activates B cell–intrinsic MyD88 signaling to promote germinal center responses and IgE/IgG1 production.

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B Cell–Intrinsic MyD88 Signaling Is Essential for IgE Responses in Lungs Exposed to Pollen Allergens

Matsushita

Yoshimoto

2014

The Journal of Immunology

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“…Об-суждается их участие в распознавании не только патогенов, но и аллергенов, токсических веществ и др. [7]. В результате активации TLRs происходит запуск каскада сигнальных молекул, приводящих к экспрессии генов про-и противовоспалитель-ных цитокинов, хемокинов, костимуляторных молекул.…”

Section: Discussionunclassified

Expression Features of Toll-Like Receptor 2 and Toll-Like Receptor 4 in Children With Asthma

et al. 2017

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Резюме. В настоящее время в литературе активно обсуждается неоднозначная роль распознаю-щих рецепторов врожденного иммунитета, в частности TLRs, в иммунопатогенезе бронхиальной астмы (БА).Цель нашей работы -исследование экспрессии ТLR2 и TLR4 на уровне клеток слизистой полости носа и лейкоцитов периферической крови (ЛПК) больных БА разной степени тяжести.В исследование были включены 40 детей с БА (3-12 лет) и 10 здоровых детей того же возраста. Методом ПЦР-РВ оценивали экспрессию генов TLR2 и TLR4 в соскобах со слизистой полости носа и в ЛПК; методом проточной цитометрии определяли процент моноцитов, лимфоцитов и гранулоци-тов, экспрессирующих TLR2 TLR4, и интенсивность их экспрессии; методом мультиплексного им-мунофлуоресцентного анализа оценивали уровень про-и противовоспалительных цитокинов (IL-1β, IL-1ra, IL-6, IL-8, IL-10, TNFα) в назальных смывах.В результате проведенного исследования выявлена гиперактивация факторов врожденного имму-нитета на уровне слизистой оболочки полости носа у больных с БА, проявляющаяся повышением экспрессии генов TLR2, TLR4 и выработки как провоспалительных, так и противовоспалительных цитокинов. Выявлена связь между уровнем цитокинов и степенью тяжести бронхиальной астмы. В периферической крови определено достоверное увеличение экспрессии TLR2 и TLR4 на циркули-рующих CD14 + моноцитах у детей с БА. Таким образом, показано увеличение экспрессии генов TLRs слизистой полости носа и повыше-ние поверхностной экспрессии TLR2 и TLR4 на циркулирующих моноцитах больных бронхиальной астмой по сравнению со здоровыми детьми. Выявленные изменения свидетельствуют о вовлечении системы TLRs в иммунопатогенез бронхиальной астмы. В дальнейшем TLRs могут быть использова-ны в качестве маркеров прогноза течения БА и возможных терапевтических мишеней.

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“…Incremental HSP expression is regulated at various levels such as RNA synthesis, mRNA stability, and translation efficiency (Mahmood et al, 2010). In recent years, proteins with immunological functions have received increasing amounts of attention; thus, focus has been placed on HSPs, which are potent activators of the innate immune system in all animals (Bezemer et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Molecular cloning and expression analysis of heat shock protein 20 (HSP20) from the pearl oyster Pinctada martensii

Lei¹,

Wu²,

Liang³

et al. 2016

Genet. Mol. Res.

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ABSTRACT. Small heat shock proteins (HSPs) are molecular chaperones with ATP-independent properties. They are involved in a variety of physiological and stress processes. In this study, the full-length HSP 20 (HSP20) from Pinctada martensii, designated as PmHSP20, was obtained from hemocytes using rapid amplification of cDNA ends technology. The PmHSP20 cDNA was 952 bp in length, containing an open reading frame of 534 bp that encoded 177-amino acid residues, with an isoelectric point of 5.86 and molecular weight of 20.24 kDa. The sequence of this deduced polypeptide contained typical structure and function domains conserved in the HSP20 family, providing evidence that PmHSP20 belongs to the HSP20 family. The PmHSP20 mRNA expression levels were detected in various tissues of P. martensii and in hemocytes after challenges with the bacteria Vibrio harveyi and lipopolysaccharide (LPS) using quantitative realtime polymerase chain reaction amplification. The results indicated that PmHSP20 is constitutively expressed in all tissues tested and might be involved in the immune response. The upregulation of PmHSP20 after V. harveyi and LPS challenge suggests that PmHSP20 plays an important role in anti-bacterial immunity. Studies on PmHSP20 are a valuable resource to further explore the immune system in pearl oysters and might enhance our knowledge of molluscan innate immunity.

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Dual Role of Toll-Like Receptors in Asthma and Chronic Obstructive Pulmonary Disease

Cited by 100 publications

References 291 publications

B Cell–Intrinsic MyD88 Signaling Is Essential for IgE Responses in Lungs Exposed to Pollen Allergens

B Cell–Intrinsic MyD88 Signaling Is Essential for IgE Responses in Lungs Exposed to Pollen Allergens

Expression Features of Toll-Like Receptor 2 and Toll-Like Receptor 4 in Children With Asthma

Molecular cloning and expression analysis of heat shock protein 20 (HSP20) from the pearl oyster Pinctada martensii

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