Dual RNA-seq reveals the effect of the flgM gene of Pseudomonas plecoglossicida on the immune response of Epinephelus coioides

Sun, Yujia; Zhuang, Zhixia; Wang, Xiaoru; Huang, Huabin; Fu, Qi; Yan, Qingpi

doi:10.1016/j.fsi.2019.01.041

Cited by 40 publications

(15 citation statements)

References 38 publications

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“…Dual RNA-seq technology is beginning to be employed to simultaneously monitor global changes between host and pathogen [23,26,27]. In the present study, during infection, the deficient strain of fliA showed blocked flagellar synthesis, and expression of Toll-like receptor 5 ( tlr5 ) was decreased, as expected.…”

Section: Discussionsupporting

confidence: 74%

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Dual RNA-Seq Unveils the Role of the Pseudomonas plecoglossicida fliA Gene in Pathogen-Host Interaction with Larimichthys crocea

et al. 2019

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In the present study, Larimichthys crocea and Pseudomonas plecoglossicida were selected as a host-pathogen interaction model for teleosts and prokaryotic pathogens. Five shRNAs were designed and synthesized to silence the fliA gene, all of which resulted in pronounced reductions in fliA mRNA; the mutant strain with the best silencing efficiency of 92.16% was chosen for subsequent analysis. A significant decrease in motility, intracellular survival and escape was observed for the fliA-RNAi strain of P. plecoglossicida, whereby silencing of the fliA gene led to a 30% decrease in mortality and a four-day delay in the onset of infection in L. crocea. Moreover, silencing of P. plecoglossicida fliA resulted in a significant change in both the pathogen and host transcriptome in the spleens of infected L. crocea. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis of pathogen transcriptome data showed that silencing fliA resulted in downregulation of 18 flagellum-related genes; KEGG analysis of host transcriptome data revealed that infection with the fliA-RNAi strain caused upregulation of 47 and downregulation of 106 immune-related genes. These pathogen-host interactions might facilitate clearance of P. plecoglossicida by L. crocea, with a significant decrease in fliA-RNAi P. plecoglossicida strain virulence in L. crocea.

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Section: Discussionsupporting

confidence: 74%

“…To win this life-and-death struggle, both pathogen and host must mobilize all available resources, and all changes will be reflected in their respective transcriptome profiles [22]. Dual RNA-seq offers the ability to monitor host-pathogen RNA expression profiles simultaneously [23,24,25,26,27].…”

Section: Introductionmentioning

confidence: 99%

Dual RNA-Seq Unveils the Role of the Pseudomonas plecoglossicida fliA Gene in Pathogen-Host Interaction with Larimichthys crocea

et al. 2019

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“…Thus, an unbiased and global understanding of dynamic changes of the pathogen transcriptomes during infection is fundamental for comprehensive understanding of pathogenic mechanism of pathogen (Colgan, Cameron, & Kröger, ; Tian, Tong, Feng, Wanghe, & Zhao, ; Tso & Lu, ). As a sensitive tool, RNA‐seq has been used to study global gene expression of the pathogen in in vitro infection systems and provides new insights into infection biology (Colgan et al, ; Sun et al, ; Westermann, Gorski, & Vogel, ).…”

Section: Introductionmentioning

confidence: 99%

clpV is a key virulence gene during in vivo Pseudomonas plecoglossicida infection

Luo

Zhao

et al. 2019

Journal of Fish Diseases

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Interaction between bacterial pathogen and aquatic animal host is exceedingly complex, which involves large dynamic changes in gene expression during different stages of the disease. However, research on identifying key virulence genes based on the dynamics of gene expression changes of a one‐sided bacterial pathogen in tissue has not been reported so far across different stages of infectious disease. The clpV for the T6SS of Pseudomonas plecoglossicida was identified for a candidate for key virulence gene based on dynamic changes of gene expression. For the Epinephelus coioides infected using clpV‐RNAi strain, no deaths were observed up to 20 dpi. The spleens, kidneys and livers of all the E. coioides that received clpV‐RNAi strain failed to develop visible nodules at 5–8 dpi, with the swelling gradually disappearing. The burdens of clpV‐RNAi strain in the spleen and blood were greatly reduced at most of the time points after injection, and the burdens of clpV‐RNAi strain in the head kidneys and trunk kidneys also had a sharp reduction from 72 to 120 hpi. This paper provides a new insight into the discovery of key virulence genes of pathogens in infected tissue systems.

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“…By contrast, P. plecoglossicida strain PQLYC4 could initiate the VWND and 100% cumulative mortality in large yellow croakers at 12℃ (Figure 4). These results indicated that P. plecoglossicida strain PQLYC4 may have a wider temperature range of infection than the strains NZBD9 and NZBD11 (Sun et al., 2019; Tang et al., 2020). Previous study has revealed that the water temperature affected the infection of P. plecoglossicida by multiple virulence mechanisms (Huang, Liu, et al, 2018).…”

Section: Discussionmentioning

confidence: 91%

An outbreak of visceral white nodules disease caused by Pseudomonas plecoglossicida at a water temperature of 12°C in cultured large yellow croaker (Larimichthys crocea) in China

Wang

Ren

et al. 2020

Journal of Fish Diseases

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The large yellow croaker (Larimichthys crocea) is an economically important maricultured fish in China, with the highest annual production among maricultured fish species (Ao et al., 2015). With the rapid development of large yellow croaker farming, the bacterial diseases are becoming more and more serious (Mu et al., 2018). At present, the most serious bacterial disease is visceral white nodules disease (VWND) characterized by white nodules in the internal organs, including spleen, kidney and liver, of infected fish. The VWND has caused high mortality in the cage-cultured large yellow croaker, resulting in significant economic losses (Zhang, Zhou, An, Chen, & Wang, 2014). As early as 2002, the VWND was epidemic in cultured large yellow croaker in Fujian, China, with a high mortality rate of about 70%-80% (Liu, Yu, Lin, Chen, & Xie, 2004). The obvious symptoms included many white nodules with a diameter of 1-2 mm observed in spleen, kidney and liver, congestion in mandible and gill cover, and inflammation in intestine. Two Pseudomonas strains (BP-1 and BS-2) were isolated and identified as the aetiological agents of this disease (Liu et al., 2004). In 2005, an outbreak of the VWND occurred in farmed large yellow croaker in Zhejiang, China, and its pathogen was identified as Pseudomonas putida (Shen

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Dual RNA-seq reveals the effect of the flgM gene of Pseudomonas plecoglossicida on the immune response of Epinephelus coioides

Cited by 40 publications

References 38 publications

Dual RNA-Seq Unveils the Role of the Pseudomonas plecoglossicida fliA Gene in Pathogen-Host Interaction with Larimichthys crocea

Dual RNA-Seq Unveils the Role of the Pseudomonas plecoglossicida fliA Gene in Pathogen-Host Interaction with Larimichthys crocea

clpV is a key virulence gene during in vivo Pseudomonas plecoglossicida infection

An outbreak of visceral white nodules disease caused by Pseudomonas plecoglossicida at a water temperature of 12°C in cultured large yellow croaker (Larimichthys crocea) in China

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