Dual RNA and 16S ribosomal DNA sequencing reveal arbuscular mycorrhizal fungi-mediated mitigation of selenate stress in Zea mays L. and reshaping of soil microbiota

Sun, Chenyu; Qiao, Guo; Zeeshan, Muhammad; Milham, Paul J.; Qin, Shengfeng; Ma, Junqing; Yang, Yisen; Lai, Hung-Yu; Huang, Jinghua

doi:10.1016/j.ecoenv.2022.114217

Cited by 5 publications

(4 citation statements)

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“…For example, biotic stress caused by Botrytis cinerea in lettuce induced the activity of CAT, SOD, and glutathione [ 43 ], while Fusarium culmorum in wheat induced the activity of CAT and POD [ 44 ]. Suillus luteus increased the activities of CAT, APX, and SOD in leaves of Quercus acutissima under Cd stress [ 45 ], while Bovistalmosa triggered the antioxidant defense of Populus yunnanensis by enhancing the activities of SOD and CAT [ 29 ]. Consistent with this work, our results also revealed that CG1 and CG2 significantly increased the antioxidant enzyme CAT, POD, and SOD activities in the shoot of Massion’s pine by 60%, 225%, and 9% and in the root by 168%, 200%, and 11% on average, respectively ( Figure 6 ).…”

Section: Discussionmentioning

confidence: 99%

“…Next, 0.2 g of leaf and root samples was extracted with 0.05 M of sodium phosphate buffer (PBS, PH7.0) and centrifuged at 15,000× g for 15 min at 4 • C. The supernatant (enzyme extract) was used to determine the activity of peroxidase (POD), catalase (CAT), superoxide dismutase (SOD), and malondialdehyde (MDA). The SOD activity was analyzed by Microplate Reader (SpectraMax iD5, Molecular Devices, SV, USA) at 560 nm [51], the POD activity was measured at 470 nm [45], while CAT was measured at 240 nm [52]. The MDA concentration was determined by the thiobarbituric acid method, while the absorbance of the supernatant was read at 532 nm and 600 nm against a reagent blank [51].…”

Section: Determination Of Photosynthetic Characteristics Antioxidant ...mentioning

confidence: 99%

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Increased Tolerance of Massion’s pine to Multiple-Toxic-Metal Stress Mediated by Ectomycorrhizal Fungi

Zhang,

Pang

et al. 2023

Plants

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Pinus massoniana (Massion’s pine), a pioneer tree species, exhibits restoration potential in polluted mining areas. However, the physiological and molecular mechanisms of ectomycorrhizal (ECM) fungi in Massion’s pine adaptability to multiple-toxic-metal stress are still unclear. Hence, Massion’s pine seedlings inoculated with two strains of C. geophilum, which were screened and isolated from a polluted mine area, were cultivated in mine soil for 90 days to investigate the roles of EMF in mediating toxic metal tolerance in host plants. The results showed that compared with the non-inoculation control, C. geophilum (CG1 and CG2) significantly promoted the biomass, root morphology, element absorption, photosynthetic characteristics, antioxidant enzyme activities (CAT, POD, and SOD), and proline content of Massion’s pine seedlings in mine soil. C. geophilum increased the concentrations of Cr, Cd, Pb, and Mn in the roots of Massion’s pine seedlings, with CG1 significantly increasing the concentrations of Pb and Mn by 246% and 162% and CG2 significantly increasing the concentrations of Cr and Pb by 102% and 78%. In contrast, C. geophilum reduced the concentrations of Cr, Cd, Pb, and Mn in the shoots by 14%, 33%, 27%, and 14% on average, respectively. In addition, C. geophilum significantly reduced the transfer factor (TF) of Cr, Cd, Pb, and Mn by 32–58%, 17–26%, 68–75%, and 18–64%, respectively, and the bio-concentration factor (BF) of Cd by 39–71%. Comparative transcriptomic analysis demonstrated that the differently expressed genes (DEGs) were mainly encoding functions involved in “transmembrane transport”, “ion transport”, “oxidation reduction process”, “oxidative phosphorylation”, “carbon metabolism”, “glycolysis/gluconeogenesis”, “photosynthesis”, and “biosynthesis of amino acids.” These results indicate that C. geophilum is able to mitigate toxic metals stress by promoting nutrient uptake, photosynthesis, and plant growth, thereby modulating the antioxidant system to reduce oxidative stress and reducing the transport and enrichment of toxic metals from the root to the shoot of Massion’s pine seedlings.

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Section: Discussionmentioning

confidence: 99%

Section: Determination Of Photosynthetic Characteristics Antioxidant ...mentioning

confidence: 99%

Increased Tolerance of Massion’s pine to Multiple-Toxic-Metal Stress Mediated by Ectomycorrhizal Fungi

Zhang,

Pang

et al. 2023

Plants

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“…Identifying the genes and transcription factors of both AMF and host plants that enable the AMF to react to diverse environmental stresses has become an essential task for researchers in this field for the future (Zou et al, 2020;Sun et al, 2022). Such investigations would enable the development of mycorrhizal partnerships with a maximum positive effect on host plants (Guo et al, 2021;Grünfeld et al, 2022).…”

Section: Amf and Micronutrient Availabilitymentioning

confidence: 99%

The inevitability of arbuscular mycorrhiza for sustainability in organic agriculture—A critical review

George

Ray²

2023

Front. Sustain. Food Syst.

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The arbuscular mycorrhizal fungi (AMF) are significant fertility-promoting microbes in soils. They enable soil fertility, soil-health and boost crop productivity. There are generalist and specialist groups among AMF in natural soils. Optimized use of specific AMF concerning crops and soils can improve agricultural sustainability. Thus, AMF is becoming an inevitable biological tool for improving crop productivity and soil health. Especially in the context of chemicalized agriculture undermining the sustainability of food security, safety, and human and ecosystem health, alternative agricultural means have become inevitable. Therefore, AMF has become essential in nature-friendly, organic agriculture. Of such farm fields, natural biological activity is enhanced to sustain soil fertility. Crops show increased innate immunity against pests and diseases in many such systems. Moreover, ecosystems remain healthy, and the soil is teeming with life in such farms. The primary goal of the review was a thorough critical analysis of the literature on AMF in organic agriculture to assess its efficiency as an ecotechnological tool in sustainable agricultural productivity. The novelty is that this is the first comprehensive review of literature on AMF concerning all aspects of organic agriculture. A vital systematic approach to the exhaustive literature collected using regular databases on the theme is followed for synthesizing the review. The review revealed the essentiality of utilizing specific mycorrhizal species, individually or in consortia, in diverse environmental settings to ensure sustainable organic crop production. However, for the exact usage of specific AMF in sustainable organic agriculture, extensive exploration of them in traditional pockets of specific crop cultivations of both chemical and organic fields and wild environments is required. Moreover, intensive experimentations are also necessary to assess them individually, in combinations, and associated with diverse beneficial soil bacteria.

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“…However, recently, it was found that microorganisms played an important role in the Se accumulation in plants. The arbuscular mycorrhizal fungi (AMF), especially Rhizophagus intraradices , were demonstrated to significantly increase Se uptake of preanthesis (selenate treatment) and postanthesis (selenite treatment) in winter wheat ( Triticum aestivum L.) [7], and could also alleviate Se stress in maize ( Zea mays L.) [8]. Two bacterial strains ( Lysinibacillus xylanilyticus and Lysinibacillus macrolides ), isolated from naturally occurring Se‐rich soils in tea gardens in China, were able to completely reduce selenite (initial as 1.0 mmol/L) to Se nanoparticles within 36 h [9].…”

Section: Introductionmentioning

confidence: 99%

A novel selenite‐tolerant rhizosphere bacterium Wautersiella enshiensis sp. nov., isolated from Chinese selenium hyperaccumulator, Cardamine hupingshanensis

Yuan

Xia

2023

J Basic Microbiol

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Selenium (Se) is a dietary essential trace element for humans with various physiological functions and it could also be accumulated by some plant species, like Astragalus bisulcatus, Stanleya pinnata, and Cardamine hupinshanensis. A novel Gram‐stain‐negative, facultatively anaerobic, selenite‐tolerant bacterium, designated strain YLX‐1T, was isolated from the rhizosphere of a Se hyperaccumulating plant, Cardamine hupingshanensis in Enshi, China. Phylogenetic analysis based on 16 S rRNA gene sequences indicated that strain YLX‐1T is a potential new species in the genus Wautersiella. Strain YLX‐1T could grow in the temperature range of 4–37°C (optimally at 28°C) and in the pH range of 5–9 (optimum pH 7), which also could tolerate Se up to 6000 mg Se/L via producing extracellular red nano‐Se with 100–300 nm size. However, it could predominantly accumulate selenocystine (SeCys2) in the cell under lower Se stress (1.5 mg Se/L). These results would help broaden our knowledge about the Se accumulation and transformation mechanism involved in rhizosphere bacteria like strain YLX‐1T in C. hupingshanensis. Based on polyphasic data, we propose the creation of the new species Wautersiella enshiensis sp. nov., strain YLX‐1T ( = CCTCC M 2013671) which will be promising to produce nano‐Se as fertilizer, food additives or medicine.

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Dual RNA and 16S ribosomal DNA sequencing reveal arbuscular mycorrhizal fungi-mediated mitigation of selenate stress in Zea mays L. and reshaping of soil microbiota

Cited by 5 publications

References 75 publications

Increased Tolerance of Massion’s pine to Multiple-Toxic-Metal Stress Mediated by Ectomycorrhizal Fungi

Increased Tolerance of Massion’s pine to Multiple-Toxic-Metal Stress Mediated by Ectomycorrhizal Fungi

The inevitability of arbuscular mycorrhiza for sustainability in organic agriculture—A critical review

A novel selenite‐tolerant rhizosphere bacterium Wautersiella enshiensis sp. nov., isolated from Chinese selenium hyperaccumulator, Cardamine hupingshanensis

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