Dual-Responsive Surfaces Modified with Phenylboronic Acid-Containing Polymer Brush To Reversibly Capture and Release Cancer Cells

Abstract

Artificial stimuli-responsive surfaces that can mimic the dynamic function of living systems have attracted much attention. However, there exist few artificial systems capable of responding to dual- or multistimulation as the natural system does. Herein, we synthesize a pH and glucose dual-responsive surface by grafting poly(acrylamidophenylboronic acid) (polyAAPBA) brush from aligned silicon nanowire (SiNW) array. The as-prepared surface can reversibly capture and release targeted cancer cells by precisely controlling pH and glucose concentration, exhibiting dual-responsive AND logic. In the presence of 70 mM glucose, the surface is pH responsive, which can vary from a cell-adhesive state to a cell-repulsive state by changing the pH from 6.8 to 7.8. While keeping the pH at 7.8, the surface becomes glucose responsive--capturing cells in the absence of glucose and releasing cells by adding 70 mM glucose. Through simultaneously changing the pH and glucose concentration from pH 6.8/0 mM glucose to pH 7.8/70 mM glucose, the surface is dual responsive with the capability to switch between cell capture and release for at least 5 cycles. The cell capture and release process on this dual-responsive surface is noninvasive with cell viability higher than 95%. Moreover, topographical interaction between the aligned SiNW array and cell protrusions greatly amplifies the responsiveness and accelerates the response rate of the dual-responsive surface between cell capture and release. The responsive mechanism of the dual-responsive surface is systematically studied using a quartz crystal microbalance, which shows that the competitive binding between polyAAPBA/sialic acid and polyAAPBA/glucose contributes to the dual response. Such dual-responsive surface can significantly impact biomedical and biological applications including cell-based diagnostics, in vivo drug delivery, etc.

Enhanced Melanoma‐Targeted Therapy by “Fru‐Blocked” Phenyboronic Acid‐Modified Multiphase Antimetastatic Micellar Nanoparticles

“…The comparison of cellular accumulation efficiency between LT NPs and PLT NPs was evaluated on B16F10 and HepG2 cells overexpressing SA residues and COS‐7 cells with low expression of SA residues 40. The cellular uptake of DOX from PLT/DOX NPs by B16F10 ( Figure 2 A) and HepG2 cells was significantly higher than that from LT/DOX NPs ( p < 0.01) (Figures S8 and S9, Supporting Information), while there was no significant difference observed with COS‐7 cells (Figures S10 and S11, Supporting Information).…”

Interfacial assembly of mesoporous nanopyramids as ultrasensitive cellular interfaces featuring efficient direct electrochemistry

“…[1][2][3][4] External signals of different types (for example, optical, electrical, magnetic, mechanical and chemical/biochemical inputs [5][6][7][8][9] ) are applied to reversibly activate nanodevice interfaces upon demand. [10][11][12][13] From these studies, molecular-semiconductor hetero-interfaces (such as protein-TiO 2 , 14,15 DNA-quantum dots, 16,17 organic molecule-silicon, 18 redox molecule-nanowires 19 and photoactive molecule-nanowires 20 ) have been applied to adjust photoelectrochemical (PEC) activities by enhancing the efficiency of PEC conversion. [21][22][23][24] In PEC-based bioanalysis (for example, DNA analysis, [25][26][27] immunoassays [28][29][30][31] and enzymatic sensing 32 ), PECenzymatic sensing has been the focus of considerable research because of the facile and inexpensive fabrication of appropriate interfaces and, more importantly, their high sensitivities and specificities.…”

Aptamer-polymer functionalized silicon nanosubstrates for enhanced recovered circulating tumor cell viability and in vitro chemosensitivity testing

“…CTCs provide an opportunity to noninvasively monitor cancer patients' prognosis and response to therapy. Over the past few decades, although great endeavor has been devoted to developing CTC capture and recovery devices in which captured cells are released usually under certain special stimuli (such as light, electric current, enzymes, temperature, and pH), [7][8][9][10][11] these techniques are often limited by a capture and recovery performance tradeoff between high efficiency and high viability. Hence, there is a desperate need for techniques to capture and recover high viability CTCs.…”