Dual proteomics of<i>Drosophila melanogaster</i>hemolymph infected with the heritable endosymbiont<i>Spiroplasma poulsonii</i>

Masson, Florent; Rommelaere, Samuel; Marra, Alice; Schüpfer, Fanny; Lemaître, Bruno

doi:10.1101/2021.02.22.432267

Cited by 4 publications

(1 citation statement)

References 67 publications

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“…Of them, two innate immune genes are dependent on upd2 for expression since they are not upregulated in a upd2Δ background. Significantly SPH93 is secreted in the extracellular space and required for gram-positive bacteria response [77][78][79]. IBIN, which encodes a long non-coding RNA (lncRNA) induced upon infection, also required Upd2 to express starved states (Figure 6B).…”

Section: How Does the Nuclear Retention Of Upd2 Exert Systemic Effect...mentioning

confidence: 99%

Nuclear Retention of Leptin/Upd2 Regulates Organismal Resilience to Nutrient Extremes

et al. 2021

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Adipocytes release adipokines to function as a systemic ‘adipometer’ and signal satiety. For organisms to function effectively, adipocytes must switch adeptly between adipokine release and retention. We investigated how Unpaired2 (Upd2), a Drosophila ortholog of the human adipokine Leptin, is retained during fasting. Unexpectedly, we find that fasting induces Upd2 nuclear accumulation; and that Upd2 nuclear retention is regulated by Atg8. Atg8 is a ubiquitin like protein which conjugates to a lipid moiety. In fed cells, Atg8 localizes both to the nucleus and the cytoplasm. On fasting Atg8 lipidation levels increase, and the nuclear pool of Atg8 is depleted. We find that Atg8 lipidation is the rate limiting step in Upd2 nuclear exit. Illustrating the physiological significance of Atg8-mediated adipokine nuclear retention, we show that this mechanism promotes starvation resilience and post-fasting hunger. Our findings uncover a new facet of Atg8 biology in regulating context-dependent nuclear export and protein localization.

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Section: How Does the Nuclear Retention Of Upd2 Exert Systemic Effect...mentioning

confidence: 99%

Nuclear Retention of Leptin/Upd2 Regulates Organismal Resilience to Nutrient Extremes

et al. 2021

Preprint

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Jellyfish blooms Through the Microbial Lens: Temporal Changes, Cross-Species and Jellyfish-Water Comparisons

Barak,

Brekhman,

Aharonovich

et al. 2024

Preprint

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In this study, we explore the dynamics of bacterial communities associated withRhopilema nomadicablooms, the predominant jellyfish in the Eastern Mediterranean Sea. We collected over 120 samples from more than 30 individuals across five major bloom events, capturing both lesser-studied winter blooms and the peaks and declines of summer blooms. Our analysis revealed significant microbial shifts-increases inEndozoicomonasand unclassifiedRickettsialeswere significantly more abundance during late summer blooms, whileTenacibaculumdominated in winter. Additionally, we examined microbial patterns within specific tissues—bell, gonads, tentacles, and gastrovascular system—to assess variations across these different niches. This revealed high relative abundance of specific taxa tailored to different tissue-Bacteroideswas predominantly found in the bell,Simkaniaceaein the gonads, andEndozoicomonasin the tentacles. Further expanding our research, we compared the top taxa ofR. nomadicawith those of nine other jellyfish species from different locations. Interestingly, while no universal core microbiome was found, several taxa, includingEndozoicomonas,Mycoplasma, andSpiroplasma, were common across different species, suggesting their potential ecological roles across jellyfish. Lastly, our study of potential bacterial transmission modes revealed that key bacteria associated withR. nomadicaare exclusively found near bloom areas, and are absent from remote seawater, highlighting potential localized transmission dynamics between jellyfish and their immediate marine environment. Our study marks the first exploration of microbial dynamics withinR. nomadica, while also broadening the understanding of other jellyfish microbial communities and setting the stage for future studies to delve deeper into their complex interactions.

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Unraveling the Role of Antimicrobial Peptides in Insects

Stączek

Cytryńska

Zdybicka-Barabas

2023

IJMS

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Antimicrobial peptides (AMPs) are short, mainly positively charged, amphipathic molecules. AMPs are important effectors of the immune response in insects with a broad spectrum of antibacterial, antifungal, and antiparasitic activity. In addition to these well-known roles, AMPs exhibit many other, often unobvious, functions in the host. They support insects in the elimination of viral infections. AMPs participate in the regulation of brain-controlled processes, e.g., sleep and non-associative learning. By influencing neuronal health, communication, and activity, they can affect the functioning of the insect nervous system. Expansion of the AMP repertoire and loss of their specificity is connected with the aging process and lifespan of insects. Moreover, AMPs take part in maintaining gut homeostasis, regulating the number of endosymbionts as well as reducing the number of foreign microbiota. In turn, the presence of AMPs in insect venom prevents the spread of infection in social insects, where the prey may be a source of pathogens.

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Dual proteomics ofDrosophila melanogasterhemolymph infected with the heritable endosymbiontSpiroplasma poulsonii

Cited by 4 publications

References 67 publications

Nuclear Retention of Leptin/Upd2 Regulates Organismal Resilience to Nutrient Extremes

Nuclear Retention of Leptin/Upd2 Regulates Organismal Resilience to Nutrient Extremes

Jellyfish blooms Through the Microbial Lens: Temporal Changes, Cross-Species and Jellyfish-Water Comparisons

Unraveling the Role of Antimicrobial Peptides in Insects

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