Dual positional specificity of wound-responsive lipoxygenase from maize seedlings

Kim, Eun-Seon; Kim, Haeyoung; Park, Ro-Dong; Lee, Youngman; Han, Oksoo

doi:10.1078/0176-1617-00898

Cited by 13 publications

(7 citation statements)

References 7 publications

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“…2); lox induction by C 6 -components has also been observed in Arabidopsis with (E)-2-hexenal treatment (Bate and Rothstein 1998). The LOX enzyme examined in this study has an unusual dual positional specificity, producing both C-9 hydroperoxides and C-13 hydroperoxides, with the latter acting as a substrate for HPL to form C 6 -volatiles (Kim et al 2002). The absence of hpl induction (Fig.…”

Section: Discussionmentioning

confidence: 66%

“…3a). HPL catalyzes the second step in the oxylipin pathway, using 13-hydroperoxy linolenic acid as a substrate to produce C 6 -volatiles (Kim et al 2002). While the hpl gene and the products derived from the HPL enzyme, (Z)-3-hexenal, (E)-2-hexenal and (Z)-3-hexenol, were not induced with (Z)-3-hexenol or MeJA treatment at either the transcript (Fig.…”

Section: Induction Of Lox and Emission Of Productsmentioning

confidence: 96%

“…In maize, the wound-responsive LOX enzyme catalyzes the first reaction of the octadecanoid pathway, converting linolenic acid into both 9 and 13 hydroperoxy linolenic acid (Hildebrand et al 1988;Kim et al 2001Kim et al , 2002. Treatment of maize plants with (Z)-3-hexenol resulted in a transient increase in lox transcripts at 1.5 h and a sharp reduction after 12 h; with BAW damage and MeJA treatments, levels of lox mRNA increased within 1.5 h and were sustained to 24 h (Fig.…”

Section: Induction Of Lox and Emission Of Productsmentioning

confidence: 99%

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(Z)-3-Hexenol induces defense genes and downstream metabolites in maize

Farag

Ibrahim

Abd

et al. 2004

Planta

156

121

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In response to insect feeding, corn plants (Zea mays cv. Delprim) release elevated levels of volatile organic compounds (VOCs), including the C(6)-volatile (Z)-3-hexenol. The level of mRNA accumulation for a series of defense genes was monitored in response to application of (Z)-3-hexenol (50 nmol) to undamaged plants. The induction of transcripts for hpl (hydroperoxide lyase), fps (farnesyl pyrophosphate synthase), pal (phenylalanine ammonia-lyase), lox (lipoxygenase), igl (indole-3-glycerol phosphate lyase) and mpi (maize proteinase inhibitor) were compared with metabolites generated from the respective pathways. While headspace VOC analysis showed an increase in (Z)-3-hexenyl acetate and methyl salicylate with lox and pal induction, respectively, MPI accumulation was not observed with an increase in mpi transcripts. Moreover, (Z)-3-hexenol treatment did not elevate sesquiterpene emissions or activate fps transcription. Chemical labeling and bioassay experiments established that exogenous (Z)-3-hexenol can be taken up and converted to a less active acetylated form. These data indicate that (Z)-3-hexenol can serve as a signaling molecule that triggers defense responses in maize and can rapidly be turned over in planta.

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Section: Discussionmentioning

confidence: 66%

Section: Induction Of Lox and Emission Of Productsmentioning

confidence: 96%

Section: Induction Of Lox and Emission Of Productsmentioning

confidence: 99%

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(Z)-3-Hexenol induces defense genes and downstream metabolites in maize

Farag

Ibrahim

Abd

et al. 2004

Planta

156

121

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“…Essas enzimas são ativadas e oxidam os ácidos graxos linoléicos, produzindo uma determinada concentração de aldeídos e compostos voláteis que inibem a formação e o desenvolvimento de fungos em grãos (KIM et al, 2002;WRIGHT et al, 2000).…”

Section: Introductionunclassified

Comportamento de híbridos de milho inoculados com os fungos causadores do complexo grãos ardidos e associação com parâmetros químicos e bioquímicos / Behavior of Corn Hybrids Inoculated with kernel-rotting Fungi and Association to Chemical and Biochemical Parameters

Mendes

Pinho

et al. 2012

Ambiência

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ResumoO objetivo deste trabalho foi avaliar híbridos de milho oriundos de empresas sementeiras do Brasil, com e sem a inoculação dos fungos Fusarium verticilioides, Stenocarpella maydis e Stenocarpella macrospora, em dois sistemas de cultivo, convencional e sistema de semeadura direta e em dois anos agrícolas e verificar a associação com parâmetros químicos e bioquímicos, que possam estar diretamente relacionados à resistência aos fungos. As características avaliadas foram à produtividade de grãos, a porcentagem de grãos ardidos, os teores de ácidos graxos, as proteínas resistentes ao calor e a atividade da enzima lipoxigenase. A significância do contraste entre os híbridos considerados resistentes versus os híbridos considerados susceptíveis, para produtividade de grãos e porcentagem de grãos ardidos evidência a existência de genótipos com maior resistência os fungos F. verticilioides, S.maydis e S. macrospora. A produtividade de grãos e a porcentagem de grãos ardidos foram influenciadas pelo tipo de híbrido, pelas safras agrícolas e 1 Dr.; Engenheiro Agrônomo; Professor do

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“…4,5 A few naturally-occurring plant LOXs exhibit dual positional specificity; these enzymes are classified as non-traditional LOXs, because their patterns of regio-and stereoselectivity are distinct from traditional LOX enzymes. [6][7][8][9] Wound-inducible maize LOX1 is a non-traditional LOX with dual positional specificity, 6,10,11 whose pre-steady state kinetic behavior has been characterized. 12 Although the regioselectivity and stereoselectivity of traditional LOX enzymes have been characterized extensively, 13 the catalytic mechanism of non-traditional dual positional specific LOX enzymes remains controversial.…”

Section: Introductionmentioning

confidence: 99%

A Substrate Serves as a Hydrogen Atom Donor in the Enzyme-Initiated Catalytic Mechanism of Dual Positional Specific Maize Lipoxygenase-1

Huon¹,

Jang²,

Cho³

et al. 2009

Bulletin of the Korean Chemical Society

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The maize lipoxgyenase-1 is a non-traditional dual positional specific enzyme and the reaction proceeds via enzyme-initiated catalysis. Bioinformatic analysis indicated that the maize lipoxygenase-1 is structurally more similar to soybean LOX1 than pea LOXN2 in that it has an additional external loop (residues 318-351) in the carboxy-terminal catalytic domain. We analyzed the dependence of product distribution on concentration of linoleic acid and monitored the formation of hydroperoxyoctadecadienoic acid as a function of enzyme concentration. Product distribution was strongly influenced by substrate concentration, such that kinetically-controlled regioisomers were enriched and thermodynamically-controlled regioisomers were depleted at high substrate concentration. Kinetic studies indicated that the formation of hydroperoxyoctadecadienoic acid saturated rapidly in an enzyme concentration-dependent manner, which implied that reactivation by reoxidation of inactive Fe(II) failed to occur. Our results support the previously proposed enzyme-initiated catalytic mechanism of the maize lipoxgyenase-1 and reveals that a substrate molecule serves as a hydrogen atom donor in its enzyme-initiated catalysis.

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Dual positional specificity of wound-responsive lipoxygenase from maize seedlings

Cited by 13 publications

References 7 publications

(Z)-3-Hexenol induces defense genes and downstream metabolites in maize

(Z)-3-Hexenol induces defense genes and downstream metabolites in maize

Comportamento de híbridos de milho inoculados com os fungos causadores do complexo grãos ardidos e associação com parâmetros químicos e bioquímicos / Behavior of Corn Hybrids Inoculated with kernel-rotting Fungi and Association to Chemical and Biochemical Parameters

A Substrate Serves as a Hydrogen Atom Donor in the Enzyme-Initiated Catalytic Mechanism of Dual Positional Specific Maize Lipoxygenase-1

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