Dual-phase stimulated Raman scattering microscopy for real-time two-color imaging

He, Ruoyu; Xu, Yan; Zhang, Lili; Ma, Shenghong; Wang, Xu; Ye, Dan; Ji, Minhe

doi:10.1364/optica.4.000044

Cited by 94 publications

(83 citation statements)

References 39 publications

(51 reference statements)

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“…Both of them have been proven to be highly desirable for label-free imaging with even up to video-rate speed. 26−32 A consensus has been reached in the field that SRS has superseded CARS microscopy in almost all technical aspects. In particular, SRS imaging offers linear dependence on concentration and shows identical spectra to spontaneous Raman with no interference complications from the nonresonance background as in CARS.…”

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confidence: 99%

Electronic Preresonance Stimulated Raman Scattering Microscopy

Min

2018

J. Phys. Chem. Lett.

116

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Optical microscopy has generated great impact for modern research. While fluorescence microscopy provides the ultimate sensitivity, it generally lacks chemical information. Complementarily, vibrational imaging methods provide rich chemical-bond-specific contrasts. Nonetheless, they usually suffer from unsatisfying sensitivity or compromised biocompatibility. Recently, electronic preresonance stimulated Raman scattering (EPR-SRS) microscopy was reported, achieving simultaneous high detection sensitivity and superb vibrational specificity of chromophores. With newly synthesized Raman-active dyes, this method readily breaks the optical color barrier of fluorescence microscopy and is well-suited for supermultiplex imaging in biological samples. In this Perspective, we first review previous utilizations of electronic resonance in various Raman spectroscopy and microscopy. We then discuss the physical origin and uniqueness of the electronic preresonance region, followed by quantitative analysis of the enhancement factors involved in EPR-SRS microscopy. On this basis, we provide an outlook for future development as well as the broad applications in biophotonics.

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confidence: 99%

Electronic Preresonance Stimulated Raman Scattering Microscopy

Min

2018

J. Phys. Chem. Lett.

116

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“…One alternative method to pulse shaping is spectral focusing, wherein the excitation pulses are chirped through highly dispersive glass rods [25,35]. In spectral focusing, the Raman transition to be probed is chosen by the temporal delay between the pump and Stokes beams.…”

Section: Resultsmentioning

confidence: 99%

Label-free pathology by spectrally sliced femtosecond stimulated Raman scattering (SRS) microscopy

et al. 2017

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Optical “virtual biopsy” is an attractive way to improve disease diagnosis and surgical guidance. Many optical microscopy techniques have been developed to provide diagnostic information without the need for tissue sectioning or staining. Among these techniques, label-free chemical imaging is the most desirable. Recently, it has been shown that narrowband, picosecond stimulated Raman scattering (SRS) can achieve comparable morphological contrast to hematoxylin and eosin staining (H&E staining), the ‘gold standard’ of pathology. However, to translate the technique from the bench to the bedside, optimal laser sources and parameters have yet to be identified. Here we describe an improvement to the narrowband SRS microscopy techniques for label-free tissue imaging. Through spectral slicing of broadband, femtosecond pulses, we are able to maintain the same protein/lipid contrast as narrowband SRS while achieving a higher signal-to-noise ratio (SNR). Our method draws upon the benefits of femtosecond pulses (e.g. higher peak power) while preserving those of picosecond pulses (e.g. adequate spectral resolution). We demonstrate this achievement through protein/lipid signal and contrast quantification of mouse brain tissue as a function of bandwidth, and comparison with numerical simulations. Further method validation is provided through imaging of additional mouse tissues: liver, kidney, and skin.

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“…41 Second, simultaneous two-color SRS imaging method has been developed with dual-phase lock-in detection, allowing real-time virtual histology without the need of post processing. 57 Such a dual-phase modulation and detection method has also been applied for other imaging modalities such as transient absorption microscopy. 53,54 Further developments will include the miniaturization of the microscopes into handheld devices and endoscopes for in vivo applications.…”

Section: Instrumentations Of Srs Microscopymentioning

confidence: 99%

Stimulated Raman scattering microscopy for rapid brain tumor histology

Yang

Chen

2017

J. Innov. Opt. Health Sci.

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Rapid histology of brain tissues with su±cient diagnostic information has the great potential to aid neurosurgeons during operations. Stimulated Raman Scattering (SRS) microscopy is an emerging label-free imaging technique, with the intrinsic chemical resolutions to delineate brain tumors from normal tissues without the need of time-consuming tissue processing. Growing number of studies have shown SRS as a \virtual histology" tool for rapid diagnosis of various types of brain tumors. In this review, we focus on the basic principles and current developments of SRS microscopy, as well as its applications for brain tumor imaging.

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Dual-phase stimulated Raman scattering microscopy for real-time two-color imaging

Cited by 94 publications

References 39 publications

Electronic Preresonance Stimulated Raman Scattering Microscopy

Electronic Preresonance Stimulated Raman Scattering Microscopy

Label-free pathology by spectrally sliced femtosecond stimulated Raman scattering (SRS) microscopy

Stimulated Raman scattering microscopy for rapid brain tumor histology

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