Abstract:Mechanistic contributions of anaerobic microbiota to airway disease are poorly understood due to inherent limitations of existing laboratory models. To address this knowledge gap, we developed a co-culture approach that maintains an oxygen-limited apical epithelial microenvironment while host cells are oxygenated basolaterally. Reduced oxygen culture did not alter the physiology or gene expression of Calu-3 cells but sustained anaerobe-epithelial interactions for 24h without affecting bacterial or host cell vi… Show more
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