Dual-opposite end multiple injection method applied to sequential determination of Na+, K+, Ca+2, Mg+2 ions and free and total glycerol in biodiesel by capillary zone electrophoresis

Sako, Alysson V. F.; Spudeit, Daniel A.; Dupim, Maurício; Filho, Waldemar Pacheco Oliveira; Saint’Pierre, Tatiana Dillenburg; Oliveira, Marcone Augusto Leal de; Micke, Gustavo Amadeu

doi:10.1016/j.chroma.2018.07.079

Cited by 13 publications

(7 citation statements)

References 44 publications

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“…In addition, the CDC can be built with cheap materials. Regarding the detection, different techniques were applied as volumetric titration, and optical ,,,− ,,, and electrochemical detection ,, after a separation step ,,,, or derivatization procedure. ,,,− ,,,, Extraction or pretreatment chambers can be rapidly assembled into the proposed equipment, avoiding operator actions during the whole analysis. The maximum content of FGL allowed in biodiesel samples is 0.020% (w/w) , and satisfactory analytical parameters were obtained in reported works.…”

Section: Results and Discussionmentioning

confidence: 99%

“…Additionally, potential risks for analysts were reduced by using a closed system, and the automation minimized human exposure to substances and made the analytical operation less dependent on the operator. On the other hand, the proposed method was applied to commercial (soybean) and laboratory synthesized biodiesel samples (sunflower and corn) in contrast to other works in which the origin of the samples was not informed, , or the samples were only obtained from soybean oil. ,,, …”

Section: Results and Discussionmentioning

confidence: 99%

“…In addition, the CDC can be built with cheap materials. Regarding the detection, different techniques were applied as volumetric titration, 10 and optical 7,16,19,[32][33][34]36,37,39 and electrochemical detection 35,37,39 after a separation step 16,34,36,40,41 or derivatization procedure. 7,16,19,[32][33][34]36,37,39,41 Extraction or pretreatment chambers 19 can be rapidly assembled into the proposed equipment, avoiding operator actions during the whole analysis.…”

Section: Variables Screening Study By Plackett-burman Designmentioning

confidence: 99%

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Free Glycerol Sensing in Biodiesel: Automatic System Based on Sequential Enzymatic Reactions Using a Fit-for-Purpose Chemiluminescence Detection Chamber

et al. 2019

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The transesterification reaction to produce biodiesel generates a residual content of free glycerol, which may cause several problems to the engines. According to the international specification, the free glycerol determination involves a derivatization step followed by chromatographic analysis, which is done by advanced laboratories. Argentina is one of the largest biodiesel producers in the world, and part of this production is carried out by small and medium-sized producers. For those producers, this analysis is expensive and requires a considerable amount of time because there are few laboratories and they are localized far from the production site. This work proposes an alternative method for free glycerol determination in biodiesel samples taking advantage of automation, the use of an economic and available kit of enzymes, and a rapid and simple detection which was improved by the use of a fit-for-purpose chemiluminescence detection chamber designed in our laboratory. Quantification of free glycerol was performed by chemiluminescence at 425 nm after the reaction of hydrogen peroxide and luminol in the presence of Co2+. The hydrogen peroxide was generated by sequential enzymatic reactions based on the catalytic effect of glycerol kinase and glycerol phosphate oxidase. The calibration curve was linear in the 0.0045–0.0200% (w/w) range (r = 0.9910), and the sample throughput of the automatic system was 8 h–1. The geometrical configuration of the designed chemiluminescence detection chamber allowed an appropriate mixture of the reagents, due to a coupled efficient mixing system, and a highly sensitive detection (LOD value: 0.0013% (w/w)). The enzymatic method was applied for the determination of glycerol in commercial (soybean) and laboratory syntethized biodiesel samples (corn and sunflower). The trueness of the method was evaluated by comparison with the reference method (EN 14105), and a nonsignificative difference between them was observed (n = 6; P = 0.05).

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Section: Results and Discussionmentioning

confidence: 99%

Section: Results and Discussionmentioning

confidence: 99%

Section: Variables Screening Study By Plackett-burman Designmentioning

confidence: 99%

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Free Glycerol Sensing in Biodiesel: Automatic System Based on Sequential Enzymatic Reactions Using a Fit-for-Purpose Chemiluminescence Detection Chamber

et al. 2019

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“…In addition, the GC methods use high temperatures for the column oven and detector, close to the temperature limits for these components, and require thermally stable GC columns. [14][15][16][17] Although the chromatographic methods are recognized for their accuracy in glycerol quantication, these conditions make their application difficult in most analytical laboratories since they make the process more expensive and less reproducible with the derivatization of glycerol before the chromatographic analysis. 5,18 In this context, several methods for determining free glycerol in biodiesel have been proposed, aiming at a more viable, simple, and environmentally friendly.…”

Section: Introductionmentioning

confidence: 99%

A cellulose monolithic stir bar for sorptive extraction of glycerol from biodiesel

Martins,

Barros,

Silva

et al. 2024

RSC Adv.

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“…Different methods of analysis have been used to determine magnesium in several types of samples, including colorimetry (Shishov et al, 2019), liquid chromatography (Paull et al, 1997), electrochemistry (Akhter et al, 2020), capillary electrophoresis (Sako et al, 2018), flame atomic absorption spectrometry (F AAS) and graphite furnace atomic absorption spectrometry (GF AAS) (Santos et al, 2019;Seeger et al, 2019), inductively coupled plasma optical emission spectrometry (ICP OES) (Souza et al, 2019) and inductively coupled plasma mass spectrometry (ICP-MS) (Moreda-Piñeiro et al, 2018). The methods recommended by the American Society of Brewing Chemists for determining magnesium in beer samples are based on the spectrophotometry, F AAS, GF AAS and ICP OES techniques (ASBC).…”

Section: Introductionmentioning

confidence: 99%

Ecofriendly and low-cost sample preparation methods for magnesium determination in beer

et al. 2021

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Ultrasound-assisted extraction and direct analysis were compared with total digestion for magnesium determination in beer samples by flame atomic absorption spectrometry. The method for total digestion used concentrated nitric acid under plate heating. In optimized instrumental conditions, validation of the analytical method was promoted, with good linear range (0.06 to 0.5 mg L�1), low limits of detection and quantification (0.04 and 0.12 �g g�1, respectively), good precision, relative standard deviation (RSD) less than 3.4%, and accuracy (recovery levels of 91.5 to 99.0%). The characteristic concentration (C0) was 9 �g L�1. The extraction procedure was performed in a 1:1 nitric acid solution for 55 min in an ultrasonic bath at 60 �C, while the direct analysis involved a dilution of the samples in a 2% v/v nitric acid solution. The different sample preparation methods were applied to 13 beer samples and at a 95% confidence level, no significant differences were observed. Thus, direct analysis proved to be more suitable for quality control routines of beer samples in the industry.

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Dual-opposite end multiple injection method applied to sequential determination of Na+, K+, Ca+2, Mg+2 ions and free and total glycerol in biodiesel by capillary zone electrophoresis

Cited by 13 publications

References 44 publications

Free Glycerol Sensing in Biodiesel: Automatic System Based on Sequential Enzymatic Reactions Using a Fit-for-Purpose Chemiluminescence Detection Chamber

Free Glycerol Sensing in Biodiesel: Automatic System Based on Sequential Enzymatic Reactions Using a Fit-for-Purpose Chemiluminescence Detection Chamber

A cellulose monolithic stir bar for sorptive extraction of glycerol from biodiesel

Ecofriendly and low-cost sample preparation methods for magnesium determination in beer

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