Dual nuclease single-cell lineage tracing by Cas9 and Cas12a
Cheng Chen,
Yuanxin Liao,
Miao Zhu
et al.
Abstract:Single-cell lineage tracing based on CRISPR/Cas9 gene editing enables the simultaneous linkage of cell states and lineage history at a high resolution. Despite its immense potential in resolving the cell fate determination and genealogy within an organism, existing implementations of this technology suffer from limitations in recording capabilities and considerable barcode dropout. Here, we introduce DuTracer, a versatile tool, which utilizes two orthogonal gene editing systems to record deep cell lineage hist… Show more
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