Dual mode of paraxial mesoderm formation during chick gastrulation

Iimura, Tadahiro; Yang, Xuesong; Weijer, Cornelis J.; Pourquié, Olivier

doi:10.1073/pnas.0610997104

Cited by 76 publications

(86 citation statements)

References 36 publications

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“…S3 in the supplementary material). As the CNH is mostly composed of node/streak border derivatives that exit this region to medial somite regions (Selleck and Stern, 1991;Selleck and Stern, 1992;Catala et al, 1996;Psychoyos and Stern, 1996;Charrier et al, 1999;Freitas et al, 2001;Cambray and Wilson, 2007;Iimura and Pourquié, 2007), this pattern of exit is also strikingly conserved through axis elongation. It is not certain whether the progenitors of lateral somitic regions are also LTAPs in an, as yet, untested region, or whether the short-term contributing cells in the vTB make up the bulk of lateral somite progenitors.…”

Section: Progenitor Populations In the Tail Budmentioning

confidence: 99%

Localised axial progenitor cell populations in the avian tail bud are not committed to a posterior Hox identity

et al. 2008

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The outgrowth of the vertebrate tail is thought to involve the proliferation of regionalised stem/progenitor cell populations formed during gastrulation. To follow these populations over extended periods, we used cells from GFP-positive transgenic chick embryos as a source for donor tissue in grafting experiments. We determined that resident progenitor cell populations are localised in the chicken tail bud. One population, which is located in the chordoneural hinge (CNH), contributes descendants to the paraxial mesoderm, notochord and neural tube, and is serially transplantable between embryos. A second population of mesodermal progenitor cells is located in a separate dorsoposterior region of the tail bud, and a corresponding population is present in the mouse tail bud. Using heterotopic transplantations, we show that the fate of CNH cells depends on their environment within the tail bud. Furthermore, we show that the anteroposterior identity of tail bud progenitor cells can be reset by heterochronic transplantation to the node region of gastrula-stage chicken embryos.

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Section: Progenitor Populations In the Tail Budmentioning

confidence: 99%

Localised axial progenitor cell populations in the avian tail bud are not committed to a posterior Hox identity

et al. 2008

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“…Some experimental evidence supports such a possibility. For instance, medial and lateral parts of the paraxial mesoderm have distinct origins from the streak (Iimura et al, 2007;Psychoyos and Stern, 1996a;Selleck and Stern, 1991), and the extraembryonic mesoderm contains hemogenic and non-hemogenic subregions (Nakazawa et al, 2006).…”

Section: Research Articlementioning

confidence: 99%

Transcriptomic landscape of the primitive streak

Alev¹,

Wu²,

Kasukawa

et al. 2010

Development

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SUMMARYIn birds and mammals, all mesoderm cells are generated from the primitive streak. Nascent mesoderm cells contain unique dorsoventral (D/V) identities according to their relative ingression position along the streak. Molecular mechanisms controlling this initial phase of mesoderm diversification are not well understood. Using the chick model, we generated high-quality transcriptomic datasets of different streak regions and analyzed their molecular heterogeneity. Fifteen percent of expressed genes exhibit differential expression levels, as represented by two major groups (dorsal to ventral and ventral to dorsal). A complete set of transcription factors and many novel genes with strong and region-specific expression were uncovered. Core components of BMP, Wnt and FGF pathways showed little regional difference, whereas their positive and negative regulators exhibited both dorsal-to-ventral and ventral-to-dorsal gradients, suggesting that robust D/V positional information is generated by fine-tuned regulation of key signaling pathways at multiple levels. Overall, our study provides a comprehensive molecular resource for understanding mesoderm diversification in vivo and targeted mesoderm lineage differentiation in vitro.

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“…2A-C). Such electroporation targets the mesoderm as well as the neural ectoderm adjacent to the presumptive otic region (Iimura et al, 2007;Psychoyos and Stern, 1996). Overexpression of pEF-Fgf constructs caused expansion of the normal OEPD Pax2-expression domain ( Fig.…”

Section: Attenuation Of Fgf Expression Is Necessary For Otic Differenmentioning

confidence: 99%

Progressive restriction of otic fate: the role of FGF and Wnt in resolving inner ear potential

Freter

Muta²,

Mak³

et al. 2008

Development

145

221

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The development of the vertebrate inner ear is an emergent process. Its progression from a relatively simple disk of thickened epithelium within head ectoderm into a complex organ capable of sensing sound and balance is controlled by sequential molecular and cellular interactions. Fibroblast growth factor (FGF) and Wnt signals emanating from mesoderm and neural ectoderm have been shown to direct inner ear fate. However, the role of these multiple signals during inner ear induction is unclear. We demonstrate that the action of the FGFs and Wnts is sequential, and that their roles support a model of hierarchical fate decisions that progressively restrict the developmental potential of the ectoderm until otic commitment. We show that signalling by Fgf3 and Fgf19 is required to initiate a proliferative progenitor region that is a precursor to both the inner ear and the neurogenic epibranchial placodes. Significantly, we find that only after FGF action is attenuated can the subsequent action of Wnt signalling allow otic differentiation to proceed. In addition, gain and loss of function of Wnt-signalling components show a role for this signalling in repressing epibranchial fate. This interplay of signalling factors ensures the correct and ordered differentiation of both inner ear and epibranchial systems.

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Dual mode of paraxial mesoderm formation during chick gastrulation

Cited by 76 publications

References 36 publications

Localised axial progenitor cell populations in the avian tail bud are not committed to a posterior Hox identity

Localised axial progenitor cell populations in the avian tail bud are not committed to a posterior Hox identity

Transcriptomic landscape of the primitive streak

Progressive restriction of otic fate: the role of FGF and Wnt in resolving inner ear potential

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