Dual-mode detection of PARP-1 by fluorescence and chemiluminescence

Xu, Ensheng; Yang, Haitang; Wang, Zhuang; Liu, Yong; Wei, Wei; Liu, Songqin

doi:10.1016/j.snb.2020.129288

Cited by 30 publications

(22 citation statements)

References 40 publications

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“…As presented in Figure B, the higher the concentration of AG014699, the lower the relative activity of PARP-1. The half-maximum inhibitory concentration IC 50 value of 28.66 nM is obtained, which is in agreement with that measured by the gold nanocluster-based fluorescent biosensor (18.12 nM) . Similarly, the higher the concentration of AZD2461, the lower the relative activity of PARP-1 (Figure C).…”

Section: Resultssupporting

confidence: 87%

“…The half-maximum inhibitory concentration IC 50 value of 28.66 nM is obtained, which is in agreement with that measured by the gold nanocluster-based fluorescent biosensor (18.12 nM). 25 Similarly, the higher the concentration of AZD2461, the lower the relative activity of PARP-1 (Figure 3C). The IC 50 value of 1.83 nM is obtained, which is consistent with that measured by the chemiluminescent method (5 nM).…”

Section: ■ Introductionmentioning

confidence: 88%

“…Nevertheless, the noncovalent electrostatic interactions between signal molecules and PAR polymer may result in weak binding affinity and false positive . Moreover, these methods usually involve high background signals, time-consuming and labor-tedious steps for the preparation/functionalization of nanomaterials/proteins, long assay time (e.g., 16 h for the gold nanocluster-based assay, 16 h for a cationic conjugated polymer-MnO 2 nanosheet-based biosensor, 8 h for the copper nanoparticle-based electrochemical method, and 8 h for the supercharged green fluorescent protein-based FRET assay), poor sensitivity (10 –4 –10 –2 U/μL), and narrow dynamic range (2–3 orders of magnitude). Recently, boronic acid and its derivatives have been introduced for PARP-1 detection .…”

Section: Introductionmentioning

confidence: 99%

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Construction of a Dendritic Nanoassembly-Based Fluorescent Biosensor for Electrostatic Interaction-Independent and Label-Free Measurement of Human Poly(ADP-ribose) Polymerase 1 in Lung Tissues

Jiang,

Ren,

Zhang

et al. 2023

Anal. Chem.

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Poly(ADP-ribose) polymerase 1 (PARP-1) is responsible for catalyzing the creation of poly(ADP-ribose) polymer and involved in DNA replication and repair. Sensitive measurement of PARP-1 is critical for clinical diagnosis. However, the conventional electrostatic attractionbased PAPR-1 assays usually involve laborious procedures, poor sensitivity, and false positives. Herein, we demonstrate the construction of a dendritic nanoassembly-based fluorescent biosensor for electrostatic interaction-independent and label-free measurement of human PARP-1 in lung tumor tissues. When PARP-1 is present, the specific doublestranded DNA (dsDNA)-activated PARP-1 transfers the ADP-ribosyl group from nicotinamide adenine dinucleotide (NAD + )/biotinylated NAD + to the PARP-1 itself, resulting in the formation of biotinylated dsDNA-PARP-1-PAR polymer bioconjugates that can be captured by magnetic beads. Upon the addition of TdT, APE1, and NH 2 -modified Trich probe, the captured dsDNAs with dual 3′-OH termini initiate TdT-activated APE1-mediated hyperbranched amplification to produce abundant dendritic DNA nanoassemblies that can be stained by SYBR Green I to generate a high fluorescence signal. This biosensor is characterized by a template-free, electrostatic interaction-independent, high sensitivity, and label-free assay. It enables rapid (less than 3 h) measurement of PARP-1 with a limit of detection of 4.37 × 10 −8 U/μL and accurate measurement of cellular PARP-1 activity with single-cell sensitivity. Moreover, it is capable of screening potential inhibitors and discriminating the PARP-1 level in normal person tissues and lung cancer patient tissues, with great potential in PARP-1-related clinical diagnosis and drug discovery.

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Section: Resultssupporting

confidence: 87%

Section: ■ Introductionmentioning

confidence: 88%

Section: Introductionmentioning

confidence: 99%

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Construction of a Dendritic Nanoassembly-Based Fluorescent Biosensor for Electrostatic Interaction-Independent and Label-Free Measurement of Human Poly(ADP-ribose) Polymerase 1 in Lung Tissues

Jiang,

Ren,

Zhang

et al. 2023

Anal. Chem.

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“…In addition, Xu et al further developed a dual-mode (both EC and PEC), label-free strategy for the detection of PARP-1 activity through gold nanocluster (AuNCs). The AuNCs adsorbed by PAR produced both strong fluorescence and chemiluminescence by catalyzing the luminol-H 2 O 2 system, which provided higher sensitivity and stability, a wider linear range, and better biocompatibility [ 111 ]. In conclusion, these highly sensitive methods for detecting PARP-1 activity may facilitate rapid assessment of retinal light damage, providing an evidentiary basis for future evaluation of the safety of light illumination produced by optoelectronic products and medical devices.…”

Section: Discussionmentioning

confidence: 99%

PARP-1 Is a Potential Marker of Retinal Photooxidation and a Key Signal Regulator in Retinal Light Injury

Zhang

Fan

et al. 2022

Oxidative Medicine and Cellular Longevity

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Advancements in technology have resulted in increasing concerns over the safety of eye exposure to light illumination, since prolonged exposure to intensive visible light, especially to short-wavelength light in the visible spectrum, can cause photochemical damage to the retina through a photooxidation-triggered cascade reaction. Poly(ADP-ribose) polymerase-1 (PARP-1) is the ribozyme responsible for repairing DNA damage. When damage to DNA occurs, including nicks and breaks, PARP-1 is rapidly activated, synthesizing a large amount of PAR and recruiting other nuclear factors to repair the damaged DNA. However, retinal photochemical damage may lead to the overactivation of PARP-1, triggering PARP-dependent cell death, including parthanatos, necroptosis, and autophagy. In this review, we retrieved targeted articles with the keywords such as “PARP-1,” “photoreceptor,” “retinal light damage,” and “photooxidation” from databases and summarized the molecular mechanisms involved in retinal photooxidation, PARP activation, and DNA repair to clarify the key regulatory role of PARP-1 in retinal light injury and to determine whether PARP-1 may be a potential marker in response to retinal photooxidation. The highly sensitive detection of PARP-1 activity may facilitate early evaluation of the effects of light on the retina, which will provide an evidentiary basis for the future assessment of the safety of light illumination from optoelectronic products and medical devices.

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“…Although the excellent performances like bare eye identification and high sensitivity have been well obtained, the single-mode strategies based on the single optical signal are usually challenged by accuracy due to the susceptibility to the external interferences [11,12]. Combining two signals as the dual-mode detection strategy attracts much focus on account of the internal verification [13][14][15][16]. The dual-mode methods including PL/surface-enhanced Raman scattering (SERS) [17], colorimetry/PL [18], and colorimetry/SERS [19] methods have been developed for HIgG detection.…”

Section: Introductionmentioning

confidence: 99%

Photoluminescent and multi-phonon resonance Raman scattering dual-mode immunoassays based on CdS nanoparticles for HIgG detection

Wen

Ding

et al. 2022

Microchim Acta

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A dual-mode immunoassay strategy based on CdS nanoparticles as signal probes with both of photoluminescent (PL) and multi-phonon resonance Raman scattering (MRRS) properties was developed. Simplified structural design and preparation were achieved due to the intrinsic integration of PL and MRRS dual signals in the single-unit CdS nanoprobes. Human immunoglobulin G (HIgG) was sensitively and specifically detected using the proposed PL-MRRS dual-mode strategy. The linear relationship between the HIgG concentration and the intensity of 707 nm PL peaks/300 cm −1 MRRS peaks under the excitation of 488 nm laser was established. The limit of detection was 0.93 fg mL −1 for PL and 1.10 fg mL −1 for MRRS. In comparison with previous IgG detection methods, the proposed method exhibited prominent advantages in detection sensitivity and working range with good stability and repeatability. An internal self-calibration was realized which ensured the accuracy and reliability of detection results. Both results of specificity experiments and serum sample analysis further confirmed the feasibility of the designed immunoassay strategy in practical serological detection. Graphical abstract Supplementary Information The online version contains supplementary material available at 10.1007/s00604-022-05530-z.

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Dual-mode detection of PARP-1 by fluorescence and chemiluminescence

Cited by 30 publications

References 40 publications

Construction of a Dendritic Nanoassembly-Based Fluorescent Biosensor for Electrostatic Interaction-Independent and Label-Free Measurement of Human Poly(ADP-ribose) Polymerase 1 in Lung Tissues

Construction of a Dendritic Nanoassembly-Based Fluorescent Biosensor for Electrostatic Interaction-Independent and Label-Free Measurement of Human Poly(ADP-ribose) Polymerase 1 in Lung Tissues

PARP-1 Is a Potential Marker of Retinal Photooxidation and a Key Signal Regulator in Retinal Light Injury

Photoluminescent and multi-phonon resonance Raman scattering dual-mode immunoassays based on CdS nanoparticles for HIgG detection

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