Dual-modality fluorescence lifetime imaging-optical coherence tomography intravascular catheter system with freeform catheter optics

Li, Cai; Bec, Julien; Zhou, Xiaoliang; Marcu, Laura

doi:10.1117/1.jbo.27.7.076005

Cited by 2 publications

(8 citation statements)

References 30 publications

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“…While PSOCT and combined FLIm-OCT devices were previously demonstrated independently, combining FLIm with PSOCT poses unique challenges due to the need for a broadband rotary junction and DCF catheters, which can affect the PSOCT performance. Additionally, the use of DCF presents the clear benefit of an additional optical channel facilitating multimodal imaging but leads to a degradation of OCT performance due to the introduction of multipath artifacts, that has not been discussed in earlier intravascular FLIm-OCT publications [ 18 , 19 , 38 ]. Specific technical developments and careful characterization of the system’s performance were addressed in this study.…”

Section: Discussionmentioning

confidence: 99%

“…The combination of OCT and FLIm leverages the mature intravascular OCT technology and the robust rotational FLIm instrumentation developed earlier in support of clinical translation studies [ 25 , 41 ]. Both modalities can be integrated using a single double-clad fiber with dimensions identical to regular single-mode fibers, as demonstrated by a few earlier studies [ 18 , 19 , 38 ]. While the FLIm-PSOCT catheter system reported here differs from standard OCT due to the addition of polarization modulation of the light directed to the tissue and polarization-diverse detection, the current implementation can achieve similar A-scan rates as a standard OCT system.…”

Section: Discussionmentioning

confidence: 99%

“…The MDU’s design was derived from an earlier implementation [ 19 ], with some key differences. Briefly, optical coupling between PSOCT and FLIm engines and the catheter’s imaging core was performed using individual stationary collimators for PSOCT, FLIm excitation, and FLIm collection, aligned with a rotary collimator to which the catheter imaging core was connected ( Fig.…”

Section: Methodsmentioning

confidence: 99%

“…Earlier work conducted in ex vivo human coronary arteries has demonstrated that a combination of FLIm-derived optical parameters can identify mFC accumulation and the presence of superficial calcification and newly formed ECM [ 7 , 16 ]. Intravascular implementations in combination with intravascular ultrasound (IVUS) and OCT are a key step towards demonstrating the clinical utility of FLIm [ 17 – 19 ].…”

Section: Introductionmentioning

confidence: 99%

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Dual modality intravascular catheter system combining pulse-sampling fluorescence lifetime imaging and polarization-sensitive optical coherence tomography

Bec,

Zhou,

Villiger

et al. 2024

Biomed. Opt. Express

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The clinical management of coronary artery disease and the prevention of acute coronary syndromes require knowledge of the underlying atherosclerotic plaque pathobiology. Hybrid imaging modalities capable of comprehensive assessment of biochemical and morphological plaques features can address this need. Here we report the first implementation of an intravascular catheter system combining fluorescence lifetime imaging (FLIm) with polarization-sensitive optical coherence tomography (PSOCT). This system provides multi-scale assessment of plaque structure and composition via high spatial resolution morphology from OCT, polarimetry-derived tissue microstructure, and biochemical composition from FLIm, without requiring any molecular contrast agent. This result was achieved with a low profile (2.7 Fr) double-clad fiber (DCF) catheter and high speed (100 fps B-scan rate, 40 mm/s pullback speed) console. Use of a DCF and broadband rotary junction required extensive optimization to mitigate the reduction in OCT performance originating from additional reflections and multipath artifacts. This challenge was addressed by the development of a broad-band (UV-visible-IR), high return loss (47 dB) rotary junction. We demonstrate in phantoms, ex vivo swine coronary specimens and in vivo swine heart (percutaneous coronary access) that the FLIm-PSOCT catheter system can simultaneously acquire co-registered FLIm data over four distinct spectral bands (380/20 nm, 400/20 nm, 452/45 nm, 540/45 nm) and PSOCT backscattered intensity, birefringence, and depolarization. The unique ability to collect complementary information from tissue (e.g., morphology, extracellular matrix composition, inflammation) with a device suitable for percutaneous coronary intervention offers new opportunities for cardiovascular research and clinical diagnosis.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Dual modality intravascular catheter system combining pulse-sampling fluorescence lifetime imaging and polarization-sensitive optical coherence tomography

Bec,

Zhou,

Villiger

et al. 2024

Biomed. Opt. Express

Self Cite

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“…The addition of lifetime measurements allows it to discriminate between multiple fluorophores or tissue components. The ability to complement other imaging modalities such as confocal microscopy, OCT, etc., makes FLIM a lucrative technology [1][2][3][4] .…”

Section: Introductionmentioning

confidence: 99%

On-chip hyperspectral detectors for fluorescence lifetime imaging

Ahamed,

Rawat,

McPhillips

et al. 2024

High-Speed Biomedical Imaging and Spectroscopy IX

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Fluorescence Lifetime Imaging (FLIM) is a powerful technique that measures the decay time of fluorophores present in tissue samples alluding to their constituent molecules. FLIM has gained popularity in biomedical imaging for applications such as detecting cancerous tumors, surgical guidance, etc. However, conventional FLIM systems are limited by a reduced number of spectral bands and long acquisition time. Moreover, the large footprint, complexity, and cost of the instrumentation make it difficult for clinical applications. In this paper, we demonstrate a reconstruction-based hyperspectral detector that can resolve decay time and intensities in broad spectral ranges while providing high sensitivity, high gain, and fast response time. The hyperspectral detector is comprised of an array of efficient, ultrafast avalanche photodetectors integrated with nanophotonic structures. We utilize different nanostructures in the detectors to modulate light-matter interactions in spectral channels. This allows us to computationally reconstruct the spectral profile of the incoming fluorescence spectrum without the need for additional filters or dispersive optics. Also, the nanophotonic structures enhance efficiency (by a factor of 2 to 10 over different wavelengths) while providing fast response time. An innovative detector design has been employed to reduce the breakdown of the avalanche photodetectors to -7.8V while maintaining high gain (~50) across the spectral range. Therefore, enabling low light detection with a high signal-to-noise ratio for FLIM applications. Added spectral channels would provide valuable information about tissue materials, morphology, and disease diagnosis. Such innovative hyperspectral sensors can now be integrated on-chip capable of miniaturizing the FLIM system and making it a commercially viable tool for clinical use. This technology has the potential to revolutionize the current FLIM system with improved detection capabilities opening doors for new horizons.

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Dual-modality fluorescence lifetime imaging-optical coherence tomography intravascular catheter system with freeform catheter optics

Cited by 2 publications

References 30 publications

Dual modality intravascular catheter system combining pulse-sampling fluorescence lifetime imaging and polarization-sensitive optical coherence tomography

Dual modality intravascular catheter system combining pulse-sampling fluorescence lifetime imaging and polarization-sensitive optical coherence tomography

On-chip hyperspectral detectors for fluorescence lifetime imaging

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