Dual labelling by 2H and 15N revealed differences in uptake potential by deep roots of chicory

Chen, Guanying; Dresbøll, Dorte Bodin; Thorup‐Kristensen, Kristian

doi:10.1016/j.rhisph.2021.100368

Cited by 9 publications

(5 citation statements)

References 57 publications

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“…Still, 15 N uptake was less affected by deep resource placement than 2 H uptake during the three-week sampling period. This indicates that while we see substantial uptake of both water and N by the deep parts of the root system, uptake of the two resources is not equally limited by the low root density and the short time available for active uptake (Chen et al 2021).…”

Section: Effect Of N and Water Supply On Soil N Content And N Uptakementioning

confidence: 68%

Dynamics of deep water and N uptake under varied N and water supply

Chen

Cr²,

Dresbøll

et al. 2021

Preprint

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Aims: Enhanced nitrogen (N) and water uptake from deep soil layers may increase resource use efficiency whilst maintaining yield under stressed conditions. Winter oilseed rape (Brassica napus L.) can develop deep roots and access deep-stored resources such as N and water, while this potential has large uncertainties in variable environments. In this study, we aimed to evaluate the effects of reduced N and water supply on deep N and water uptake. Methods: Oilseed rape plants grown in outdoor rhizotrons were supplied with 240 and 80 kg N ha-1 respectively in 2019 whereas a well-watered and a water-deficit treatment were established in 2020. To track deep water and N uptake, a mixture of 2H2O and Ca(15NO3)2 was injected into the soil column at 0.5 and 1.7 m depths. δ2H in transpiration water and δ15N in leaves were measured after injection. δ15N in biomass samples was also measured. Results: Differences in N or water supply had little effect on root growth. The low N treatment reduced water uptake throughout the soil profile, but caused a non-significant increment in 15N uptake efficiency at both 0.5 and 1.7 m. Water deficit in the upper soil layers led to compensatory deep water, while N uptake was not altered by soil water status. Conclusion: Our findings demonstrate that for winter oilseed rape, high N application and water deficiency in shallow layers increase deep water uptake and that the efficiency of deep N uptake is mainly sensitive to N supply rather than water supply.

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Section: Effect Of N and Water Supply On Soil N Content And N Uptakementioning

confidence: 68%

Dynamics of deep water and N uptake under varied N and water supply

Chen

Cr²,

Dresbøll

et al. 2021

Preprint

Self Cite

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show abstract

“…They suggested that the inadequate N supply from topsoil might be the stimulator of subsoil N uptake. The differences in 2 H and 15 N uptake patterns indicate that while we see substantial uptake of both water and N by the deep parts of the root system, uptake of the two resources is not equally limited by the low root density and the short time available for active uptake (Chen et al, 2021).…”

Section: Deep Roots For Water and N Uptakementioning

confidence: 72%

“…In this way, the tracer solution was distributed into 100 individual points in the soil at each injection depth. For further details on the labeling process, refer to Chen et al (2021). The injection procedures were conducted between 1:00-4:00 pm on 3 April 2019 and 17 April 2020.…”

Section: H and 15 N Labelingmentioning

confidence: 99%

Dynamics of Deep Water and N Uptake of Oilseed Rape (Brassica napus L.) Under Varied N and Water Supply

et al. 2022

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Enhanced nitrogen (N) and water uptake from deep soil layers may increase resource use efficiency while maintaining yield under stressed conditions. Winter oilseed rape (Brassica napus L.) can develop deep roots and access deep-stored resources such as N and water to sustain its growth and productivity. Less is known of the performance of deep roots under varying water and N availability. In this study, we aimed to evaluate the effects of reduced N and water supply on deep N and water uptake for oilseed rape. Oilseed rape plants grown in outdoor rhizotrons were supplied with 240 and 80 kg N ha−1, respectively, in 2019 whereas a well-watered and a water-deficit treatment were established in 2020. To track deep water and N uptake, a mixture of 2H2O and Ca(15NO3)2 was injected into the soil column at 0.5- and 1.7-m depths. δ2H in transpiration water and δ15N in leaves were measured after injection. δ15N values in biomass samples were also measured. Differences in N or water supply had less effect on root growth. The low N treatment reduced water uptake throughout the soil profile and altered water uptake distribution. The low N supply doubled the 15N uptake efficiency at both 0.5 and 1.7 m. Similarly, water deficit in the upper soil layers led to compensatory deep water uptake. Our findings highlight the increasing importance of deep roots for water uptake, which is essential for maintaining an adequate water supply in the late growing stage. Our results further indicate the benefit of reducing N supply for mitigating N leaching and altering water uptake from deep soil layers, yet at a potential cost of biomass reduction.

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“…For example, differential injury from herbicides placed at a specific soil depth has been used to distinguish deeper rooted crop genotypes in the greenhouse (Al-Shugeairy et al, 2014) and field (Corre-Hellou & Crozat, 2005). Detection of naturally occurring or added tracers such as oxygen isotopes, heavy water, or deuterium offers estimates of depth of water acquisition (Chen et al, 2019(Chen et al, , 2021Chimungu et al, 2014aChimungu et al, , 2014bKulmatiski et al, 2017;Rothfuss & Javaux, 2017); however, isotope characterization from plant tissue and soil can be a tedious and costly process, and it may not be as straightforward as has been previously thought (Freyberg et al, 2020). Shoot accumulation of 15 N from pulses injected at specific soil depths has been used to estimate genotypic variation in the depth of N acquisition in maize (Chen et al, 2019;Chimungu et al, 2015;Saengwilai, Tian, et al, 2014;Schneider et al, 2022;Wacker et al, 2022).…”

Section: Tracer Applicationmentioning

confidence: 99%

LEADER (Leaf Element Accumulation from DEep Roots): A nondestructive phenotyping platform to estimate rooting depth in the field

Hanlon,

Brown,

Lynch

2023

Crop Science

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Deeper rooted crops are an avenue to increase plant water and nitrogen uptake under limiting conditions and increase long‐term soil carbon storage. Measuring rooting depth, however, is challenging due to the destructive, laborious, or imprecise methods that are currently available. Here, we present LEADER (Leaf Element Accumulation from DEep Roots) as a method to estimate in‐field root depth of maize plants. We use both X‐ray fluorescence (XRF) spectroscopy and ICP‐OES (inductively coupled plasma optical emission spectroscopy) to measure leaf elemental content and relate this to metrics of root depth. Principal components of leaf elemental content correlate with measures of root length in four genotypes (R2 = 0.8 for total root length), and we use linear discriminant analysis to classify plants as having different metrics related to root depth across four field sites in the United States. We can correctly classify the plots with the longest root length at depth (deeper than 30 or 40 cm) with high accuracy (accuracy >0.6) at two of our field sites (Hancock, WI and Rock Spring, PA). We also use strontium (Sr) as a tracer element in both greenhouse and field studies, showing that elemental accumulation of Sr in leaf tissue can be measured with XRF and can estimate root depth. We propose the adoption of LEADER as a tool for measuring root depth in different plant species and soils. LEADER is faster and easier than any other methods that currently exist and could allow for extensive study and understanding of deep rooting.

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Dual labelling by 2H and 15N revealed differences in uptake potential by deep roots of chicory

Cited by 9 publications

References 57 publications

Dynamics of deep water and N uptake under varied N and water supply

Dynamics of deep water and N uptake under varied N and water supply

Dynamics of Deep Water and N Uptake of Oilseed Rape (Brassica napus L.) Under Varied N and Water Supply

LEADER (Leaf Element Accumulation from DEep Roots): A nondestructive phenotyping platform to estimate rooting depth in the field

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