Dual‐labeled chemiluminescence enzyme immunoassay for simultaneous measurement of total prostate specific antigen (TPSA) and free prostate specific antigen (FPSA)

Zhao, Lixia; Wang, Dan; Gen, Shi; Li, Gang

doi:10.1002/bio.3358

Cited by 24 publications

(10 citation statements)

References 33 publications

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“…Laboratory examinations were done in the Second Hospital of Shandong University with a biochemical analyzer which is hisemikon blood cell instrument XN9000 (Roche, Basel, Switzerland). The serum PSA was measured using an automatic electrochemistry luminescence immunoassay system (CLEIA) (Roche, Basel, Switzerland) ( 18 ). Complete blood count analysis was performed immediately within half an hour after collection of blood samples into EDTA tube after venipuncture to prevent platelet activation in vitro .…”

Section: Methodsmentioning

confidence: 99%

Mean Platelet Volume Enhances the Diagnostic Specificity of PSA for Prostate Cancer

et al. 2022

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Mean platelet volume (MPV) is an indicator of platelet activation and has been proposed as a diagnostic marker for several kinds of cancers. We investigated the value of MPV as a diagnostic marker for prostate cancer (PCa) and examined whether MPV in combination with prostate-specific antigen (PSA) could increase the sensitivity or specificity of PSA for PCa diagnosis. For this study, 107 pathologically confirmed PCa and 177 non-PCa patients who underwent prostate biopsy were retrospectively studied. Clinical data and pre-biopsy hematological parameters were collected. The above parameters were compared between PCa and non-PCa patients. The correlation between MPV and clinical characteristics was analyzed. Receiver operating characteristic (ROC) analysis was used to explore the diagnostic value of MPV for PCa. Among all parameters analyzed, the difference was only found in MPV, platelet distribution width (PDW), and PSA between PCa and non-PCa patients. MPV was significantly decreased and PDW increased in PCa than that of non-PCa among men. ROC analysis identified MPV ≤ 9.05 fl as a cut-off value for potential PCa with area under the ROC curve (AUC) = 0.783, 95% CI = 0.733–0.833, sensitivity = 0.746, and specificity = 0.708. AUC and the sensitivity of MPV were comparable with total PSA (TPSA) or free PSA (FPSA). However, the specificity of MPV was larger than that of TPSA (0.461) or FPSA (0.561). Furthermore, MPV combined with TPSA or FPSA further enhanced the specificity of TPSA (0.844) or FPSA (0.927), but PDW did not. These findings suggested that MPV could have a predictive value for the diagnosis of PCa. MPV in combination with TPSA or FPSA could enhance the specificity of PSA and may reduce the rate of unnecessary biopsy for patients with high levels of PSA.

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Section: Methodsmentioning

confidence: 99%

Mean Platelet Volume Enhances the Diagnostic Specificity of PSA for Prostate Cancer

et al. 2022

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“…The detection limits are both relevant to the clinical ranges achieved using standard antibody-based immunoassays to evaluate the diagnosis or prognosis PCa patients [100]. Zhao et al used a dual-labeled CL immunoassay to simultaneously measure tPSA and fPSA from diluted human serum samples in just over 1 h [102]. A sandwich immunoassay was also used, in which capture monoclonal antibodies were first immobilized on the sensing platform.…”

Section: Chemiluminescencementioning

confidence: 99%

“…In contrast to CL, electrochemiluminescence (ECL) is electrochemically generated, and therefore electron transfer at or near the working electrode is initiated and manipulated only Zhao et al used a dual-labeled CL immunoassay to simultaneously measure tPSA and fPSA from diluted human serum samples in just over 1 h [102]. A sandwich immunoassay was also used, in which capture monoclonal antibodies were first immobilized on the sensing platform.…”

Section: Electrochemiluminescencementioning

confidence: 99%

“…Zhao et al used a dual-labeled CL immunoassay to simultaneously measure tPSA and fPSA from diluted human serum samples in just over 1 h [ 102 ]. A sandwich immunoassay was also used, in which capture monoclonal antibodies were first immobilized on the sensing platform.…”

Section: Multiplexed Detection Of Pca Protein Biomarkers Via Miniaturized Biosensor Systemsmentioning

confidence: 99%

“…The results obtained from this assay were also correlated with commercial chemiluminescent kits using clinical samples. It was concluded that this device would be useful for early diagnosis of PCa and could be used for routine clinical testing [ 102 ].…”

Section: Multiplexed Detection Of Pca Protein Biomarkers Via Miniaturized Biosensor Systemsmentioning

confidence: 99%

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Multiplexed Prostate Cancer Companion Diagnostic Devices

Aidoo-Brown

Moschou

Estrela

2021

Sensors

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Prostate cancer (PCa) remains one of the most prominent forms of cancer for men. Since the early 1990s, Prostate-Specific Antigen (PSA) has been a commonly recognized PCa-associated protein biomarker. However, PSA testing has been shown to lack in specificity and sensitivity when needed to diagnose, monitor and/or treat PCa patients successfully. One enhancement could include the simultaneous detection of multiple PCa-associated protein biomarkers alongside PSA, also known as multiplexing. If conventional methods such as the enzyme-linked immunosorbent assay (ELISA) are used, multiplexed detection of such protein biomarkers can result in an increase in the required sample volume, in the complexity of the analytical procedures, and in adding to the cost. Using companion diagnostic devices such as biosensors, which can be portable and cost-effective with multiplexing capacities, may address these limitations. This review explores recent research for multiplexed PCa protein biomarker detection using optical and electrochemical biosensor platforms. Some of the novel and potential serum-based PCa protein biomarkers will be discussed in this review. In addition, this review discusses the importance of converting research protocols into multiplex point-of-care testing (xPOCT) devices to be used in near-patient settings, providing a more personalized approach to PCa patients’ diagnostic, surveillance and treatment management.

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A Sequential Dual‐Lock Strategy for Photoactivatable Chemiluminescent Probes Enabling Bright Duplex Optical Imaging

et al. 2020

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Chemiluminescence (CL)‐based technologies have revolutionized in vivo monitoring of biomolecules. However, significant technical hurdles have limited the achievement of trigger‐controlled, bright, and enriched CL signal. Herein, a dual‐lock strategy uses sequence‐dependent triggers for bright optical imaging with real‐time fluorescent signal and ultra‐sensitive CL signal. These probes can obtain an analyte‐triggered accumulation of stable pre‐chemiluminophore with aggregation‐induced emission (AIE), and then the pre‐chemiluminophore exhibits a rapid photooxidation process (1,2‐dioxetane generation) by TICT‐based free‐radical addition, thereby achieving an enrichment and bright CL signal. The dual‐lock strategy expands the in vivo toolbox for highly accurate analysis and has for the first time allowed access to accurately sense and trace biomolecules with high‐resolution, dual‐mode of chemo‐fluoro‐luminescence, and three‐dimensional (3D) imaging in living animals.

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Dual‐labeled chemiluminescence enzyme immunoassay for simultaneous measurement of total prostate specific antigen (TPSA) and free prostate specific antigen (FPSA)

Cited by 24 publications

References 33 publications

Mean Platelet Volume Enhances the Diagnostic Specificity of PSA for Prostate Cancer

Mean Platelet Volume Enhances the Diagnostic Specificity of PSA for Prostate Cancer

Multiplexed Prostate Cancer Companion Diagnostic Devices

A Sequential Dual‐Lock Strategy for Photoactivatable Chemiluminescent Probes Enabling Bright Duplex Optical Imaging

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