Dual involvement of phytochrome in light-oriented chloroplast movement in Dryopteris sparsa protonemata

Yatsuhashi, Hiroko; Kobayashi, Hirotoshi

doi:10.1016/1011-1344(93)80089-r

Cited by 21 publications

(5 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In many cases, light-dependent intracellular movements are under the control of blue-light photoreceptor(s) (Haupt 1982;Senger 1980Senger pp.50-96, 1984. Involvement of phytochrome in such movements has been well documented in the green algae Mesotaenium and Mougeotia (Haupt 1982), and in the ferns Adiantum (Yatsuhashi et al 1985) and Dryopteris (Yatsuhashi and Kobayashi 1993). In these cases, the intracellular orientation of chloroplasts is controlled not only by phytochrome but also by blue-light photoreceptor(s).…”

Section: Discussionmentioning

confidence: 97%

“…50-96, 1984 pp. 444~62), and phytochrome has been shown to be involved in several cases (Haupt 1982;Yatsuhashi et al 1985;Yatsuhashi and Kobayashi 1993). The movement of the cytoplasmic matrix is driven by a well-organized motile apparatus, as it is in animal cells, and this apparatus is composed predominantly of microtubules, microfilaments, and the motor proteins associated with each.…”

Section: Introductionmentioning

confidence: 98%

See 1 more Smart Citation

Regulation of the orientation movement of chloroplasts in epidermal cells of Vallisneria: cooperation of phytochrome with photosynthetic pigment under low-fluence-rate light

Dong

Takagi

1995

Planta

View full text Add to dashboard Cite

Abstract. In epidermal cells of the leaves of the aquaticangiosperm Vallisneria gigantea Graebner, the chloroplasts accumulate in the outer periclinal layer of cytoplasm (P side) under light at low fluence rates. The nature of such intracellular orientation of chloroplasts was investigated in a semiquantitative manner. Time-lapse video microscopy revealed that, while irradiation with red light (650 nm, 0.41 W" m-2) rapidly accelerated the migration of chloroplasts, not only from the anticlinal layers of cytoplasm (A sides) to the P side but also from the P side to the A sides, the increased rate of migration in both directions returned to the control rate upon subsequent irradiation with far-red light (746nm, 0.14W.m-2). These effects of red and far-red light could be observed repeatedly, both in the presence and in the absence of inhibitors of photosynthesis, suggesting the involvement of phytochrome as the photoreceptor. After saturating irradiation with red light, the increased rate of migration of chloroplasts from the P side to the A sides declined more rapidly than the increased rate of migration in the opposite direction. This imbalance in the migration of chloroplasts between the two opposing directions resulted in the accumulation of chloroplasts on the P side. The more rapid decline in the rate of migration of chloroplasts from the P side to the A sides than in the opposite direction was not observed in the presence of an inhibitor of photosynthesis. It appears, therefore, that phytochrome and photosynthetic pigment cooperatively regulate the accumulation of chloroplasts on the P side through modulation of the nature of the movement of the chloroplasts.Abbreviations: A side = cytoplasmic layer that faces the anticlinal wall; DCMU = 3-(3,4-dichlorophenyl)-l,l-dimethylurea; Pfr = farred-light-absorbing form of phytochrome; Pr = red-light-absorbing form of phytochrome; P side = cytoplasmic layer that faces the outer periclinal wall Correspondence to: S. Takagi; FAX: 81 (6) 850 5817; Tel: 81 (6) 850 5818

show abstract

Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 98%

Regulation of the orientation movement of chloroplasts in epidermal cells of Vallisneria: cooperation of phytochrome with photosynthetic pigment under low-fluence-rate light

Dong

Takagi

1995

Planta

View full text Add to dashboard Cite

show abstract

“…However, the signal may persist for longer after the red light is turned off compared to that occurring after the blue light is turned, since the first red light but not blue light delayed moving to the second microbeam (either red or blue light). The Pfr form of phytochrome (i.e., the phytochrome part of neo1) reportedly has a long life time Yatsuhashi and Kobayashi 1993). Thus, the involvement of neo1 Pfr may explain the long lag time needed to respond to the second microbeam.…”

Section: Discussionmentioning

confidence: 99%

Chloroplasts do not have a polarity for light-induced accumulation movement

2008

View full text Add to dashboard Cite

Chloroplast photorelocation movement in green plants is generally mediated by blue light. However, in cryptogam plants, including ferns, mosses, and algae, both red light and blue light are effective. Although the photoreceptors required for this phenomenon have been identified, the mechanisms underlying this movement response are not yet known. In order to analyze this response in more detail, chloroplast movement was induced in dark-adapted Adiantum capillus-veneris gametophyte cells by partial cell irradiation with a microbeam of red and/or blue light. In each case, chloroplasts were found to move toward the microbeam-irradiated area. A second microbeam was also applied to the cell at a separate location before the chloroplasts had reached the destination of the first microbeam. Under these conditions, chloroplasts were found to change their direction of movement without turning and move toward the second microbeam-irradiated area after a lag time of a few minutes. These findings indicate that chloroplasts can move in any direction and do not exhibit a polarity for chloroplast accumulation movement. This phenomenon was analyzed in detail in Adiantum and subsequently confirmed in Arabidopsis thaliana palisade cells. Interestingly, the lag time for direction change toward the second microbeam in Adiantum was longer in the red light than in the blue light. However, the reason for this discrepancy is not yet understood.

show abstract

“…Light-induced chloroplast relocation movement has been studied using physiological approaches in various plant species, including green algae (Haupt et al, 1969;Kraml et al, 1988), mosses Kadota et al, 2000;Sato et al, 2001), ferns (Yatsuhashi et al, 1985;Yatsuhashi and Kobayashi, 1993;, and angiosperms (Trojan and Gabrys, 1996;Kagawa and Wada, 2000;Takagi, 2003). Recently, genetic approaches using Arabidopsis (Arabidopsis thaliana) mutants have identified the photoreceptors and genes involved in chlo-roplast movement (for review, see Suetsugu and Wada, 2007).…”

mentioning

confidence: 99%

Chloroplast Outer Envelope Protein CHUP1 Is Essential for Chloroplast Anchorage to the Plasma Membrane and Chloroplast Movement

Oikawa

Yamasato²,

Kong³

et al. 2008

Plant Physiology

164

144

View full text Add to dashboard Cite

Chloroplasts change their intracellular distribution in response to light intensity. Previously, we isolated the chloroplast unusual positioning1 (chup1) mutant of Arabidopsis (Arabidopsis thaliana). This mutant is defective in normal chloroplast relocation movement and shows aggregation of chloroplasts at the bottom of palisade mesophyll cells. The isolated gene encodes a protein with an actin-binding motif. Here, we used biochemical analyses to determine the subcellular localization of full-length CHUP1 on the chloroplast outer envelope. A CHUP1-green fluorescent protein (GFP) fusion, which was detected at the outermost part of mesophyll cell chloroplasts, complemented the chup1 phenotype, but GFP-CHUP1, which was localized mainly in the cytosol, did not. Overexpression of the N-terminal hydrophobic region (NtHR) of CHUP1 fused with GFP (NtHR-GFP) induced a chup1-like phenotype, indicating a dominant-negative effect on chloroplast relocation movement. A similar pattern was found in chloroplast OUTER ENVELOPE PROTEIN7 (OEP7)-GFP transformants, and a protein containing OEP7 in place of NtHR complemented the mutant phenotype. Physiological analyses of transgenic Arabidopsis plants expressing truncated CHUP1 in a chup1 mutant background and cytoskeletal inhibitor experiments showed that the coiled-coil region of CHUP1 anchors chloroplasts firmly on the plasma membrane, consistent with the localization of coiled-coil GFP on the plasma membrane. Thus, CHUP1 localization on chloroplasts, with the N terminus inserted into the chloroplast outer envelope and the C terminus facing the cytosol, is essential for CHUP1 function, and the coiled-coil region of CHUP1 prevents chloroplast aggregation and participates in chloroplast relocation movement.

show abstract

Dual involvement of phytochrome in light-oriented chloroplast movement in Dryopteris sparsa protonemata

Cited by 21 publications

References 7 publications

Regulation of the orientation movement of chloroplasts in epidermal cells of Vallisneria: cooperation of phytochrome with photosynthetic pigment under low-fluence-rate light

Regulation of the orientation movement of chloroplasts in epidermal cells of Vallisneria: cooperation of phytochrome with photosynthetic pigment under low-fluence-rate light

Chloroplasts do not have a polarity for light-induced accumulation movement

Chloroplast Outer Envelope Protein CHUP1 Is Essential for Chloroplast Anchorage to the Plasma Membrane and Chloroplast Movement

Contact Info

Product

Resources

About