Dual independent delivery of pro-angiogenic growth factors from starPEG-heparin hydrogels

Neurotropic growth factors and glycosaminoglycan based matrices to induce dopaminergic tissue formation

“…For GDNF an overall protein amount of 38 ± 3 ng/cm 2 for single factor and 28 ± 6 ng/cm 2 for the combination with FGF-2 were found after 168 h (P > 0.05, Student's t-test; n ¼ 3). Altogether, analogously to previous findings [37] these data impressively demonstrate that starPEG-heparin hydrogels can be applied for the independent and sustainable delivery of a FGF-2 and GDNF combination.…”

“…As already shown for FGF-2 and VEGF, combinations of cytokines can be sustainably and independently delivered by these scaffolds over a wide range of concentrations. Due to the superior effect of the parallel growth factor presentation compared to the administration of single proteins [37] a similar approach was explored herein for the precisely adjustable biomimetic uptake and release of neurotropic factors.…”

Molecular weight and concentration of heparin in hyaluronic acid-based matrices modulates growth factor retention kinetics and stem cell fate

“…Prestwich and co-workers developed covalently linked heparin-containing hyaluronic acid-based hydrogels and demonstrated efficient in vivo neovascularization due to improved loading efficiency and slow release of several heparin-binding growth factors including FGF-2, VEGF, KGF, PDGF, and TGFβ1 [20, 24–26]. More recently, heparin-containing PEG based hydrogels have been reported to act as an efficient reservoir and a tunable delivery system for various growth factors including FGF-2, VEGF, SDF-1α, and EGF, which resulted in better angiogenesis in the chicken chorioallantoic membrane (CAM), in vitro tumor, and in vivo murine kidney models [17, 18, 27, 28]. Collectively, these studies indicate that the presence of heparin in a synthetic matrix significantly enhances the encapsulation and retention of growth factors within the matrix, facilitates maintenance of their bioactivity for prolonged periods, and modulates biological response both in vitro and in vivo .…”

Integrating Mechanical and Biological Control of Cell Proliferation Through Bioinspired Multieffector Materials

“…PEG-based hydrogels, combined with covalently-linked VEGF, have shown the ability not only to stimulate cell migration and proliferation, but also to maintain their viability (116,117) . Interestingly, it has been observed, as it has previously mentioned for skeletal tissue regeneration, an improved effect of RGD motifs when administered simultaneously to two GFs in both a chicken chorioallantoic membrane (CAM) assay and in an in vivo model (117) . In line with these studies, Thomas and co-workers have developed an advanced type of PEG hydrogels for localized and sustained delivery of angiogenic factors, using immobilized lentiviruses as GF expression system.…”

“…Increased the formation of new bone in the porosities of a canine implant (151) TGF- Cyclodextrin(CD)-and Cucurbituril(CB)-based hydrogels Effective chondrogenic differentiation (115) VEGF PEG-based hydrogels Stimulates cell migration and proliferation and maintain cell viability (116,117) VEGF Embedded in gelatine-like gels…”