“Dual-functional” strontium titanate nanotubes designed based on fusion peptides simultaneously enhancing anti-infection and osseointegration

Wang, Bingbing; Bian, Anqi; Jia, Fenghuan; Lan, Jingpin; Yang, Hao; Yan, Ke; Xie, Lei; Qiao, Hongchao; Chang, Xiaotong; Lin, He; Zhang, Hui; Huang, Yong

doi:10.1016/j.msec.2022.112650

Cited by 41 publications

(12 citation statements)

References 59 publications

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“…After sulfonation and hydrothermal reaction, the water contact angle of SPEEK increased to 93.90° ± 2.43°and became more hydrophobic ( p < 0.001). After loading HHC36, the water contact angle of HSPEEK decreased significantly to 41.47° ± 0.85° and became hydrophilic ( p < 0.001), which may result from the hydrophilic residues in HHC36 ( Wang et al, 2022 ).…”

Section: Resultsmentioning

confidence: 99%

“…It exhibits better antibacterial properties than conventional antibiotics (tobramycin, ciprofloxacin, imipenem, ceftazidime) and clinical candidate AMPs (such as MX226 and hLF1-11) ( Cherkasov et al, 2009 ; Kazemzadeh-Narbat et al, 2013 ). In recent years, some reports have shown that the antibacterial properties of biomaterials have improved after the integration of HHC36 ( Lim et al, 2018 ; Chen et al, 2019 , 2020 , 2021 ; Cheng et al, 2021 ; Guo et al, 2022 ; Wang et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

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Antibacterial properties of antimicrobial peptide HHC36 modified polyetheretherketone

et al. 2023

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IntroductionPolyetheretherketone (PEEK) is considered to be a new type of orthopedic implant material due to its mechanical properties and biocompatibility. It is becoming a replacement for titanium (Ti) due to its near-human-cortical transmission and modulus of elasticity. However, its clinical application is limited because of its biological inertia and susceptibility to bacterial infection during implantation. To solve this problem, there is an urgent need to improve the antibacterial properties of PEEK implants.MethodsIn this work, we fixed antimicrobial peptide HHC36 on the 3D porous structure of sulfonated PEEK (SPEEK) by a simple solvent evaporation method (HSPEEK), and carried out characterization tests. We evaluated the antibacterial properties and cytocompatibility of the samples in vitro. In addition, we evaluated the anti-infection property and biocompatibility of the samples in vivo by establishing a rat subcutaneous infection model.ResultsThe characterization test results showed that HHC36 was successfully fixed on the surface of SPEEK and released slowly for 10 days. The results of antibacterial experiments in vitro showed that HSPEEK could reduce the survival rate of free bacteria, inhibit the growth of bacteria around the sample, and inhibit the formation of biofilm on the sample surface. The cytocompatibility test in vitro showed that the sample had no significant effect on the proliferation and viability of L929 cells and had no hemolytic activity on rabbit erythrocytes. In vivo experiments, HSPEEK can significantly reduce the bacterial survival rate on the sample surface and the inflammatory reaction in the soft tissue around the sample.DiscussionWe successfully loaded HHC36 onto the surface of SPEEK through a simple solvent evaporation method. The sample has excellent antibacterial properties and good cell compatibility, which can significantly reduce the bacterial survival rate and inflammatory reaction in vivo. The above results indicated that we successfully improved the antibacterial property of PEEK by a simple modification strategy, making it a promising material for anti-infection orthopedic implants.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Antibacterial properties of antimicrobial peptide HHC36 modified polyetheretherketone

et al. 2023

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“…LL-37 is a promising AMP to promote the proliferation, migration, and osteogenic differentiation of MSCs ( Liu H et al, 2018 ). And human β defensin 3 has also been reported to promote MSC osteogenesis ( Wang B et al, 2022 ). Furthermore, some AMPs could also inhibit osteoclastogenesis.…”

Section: Potential Effects Of Antimicrobial Peptides For Bone Tissue ...mentioning

confidence: 99%

“…In addition, titanium dioxide nanotubes are widely used in the study of surface loading of AMPs on titanium implants because of their excellent biocompatibility as carriers of biofactors and their ability to provide local slow release function ( Chen X et al, 2020 ; Wang L et al, 2022 ). Wang et al used anodic oxidation and hydrothermal synthesis to prepare uniformly distributed and well-arranged TNTs structures on the surface of titanium plates ( Wang B et al, 2022 ). The fabricated TNTs were hollow tube-like structures, which provided the structural basis for subsequent high loading of AMPs.…”

Section: Topical Applications Of Antimicrobial Peptides For Bone Tiss...mentioning

confidence: 99%

Antimicrobial peptides for bone tissue engineering: Diversity, effects and applications

Hao

Chen²,

Chai³

et al. 2022

Front. Bioeng. Biotechnol.

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Bone tissue engineering has been becoming a promising strategy for surgical bone repair, but the risk of infection during trauma repair remains a problematic health concern worldwide, especially for fracture and infection-caused bone defects. Conventional antibiotics fail to effectively prevent or treat bone infections during bone defect repair because of drug-resistance and recurrence, so novel antibacterial agents with limited resistance are highly needed for bone tissue engineering. Antimicrobial peptides (AMPs) characterized by cationic, hydrophobic and amphipathic properties show great promise to be used as next-generation antibiotics which rarely induce resistance and show potent antibacterial efficacy. In this review, four common structures of AMPs (helix-based, sheet-based, coil-based and composite) and related modifications are presented to identify AMPs and design novel analogs. Then, potential effects of AMPs for bone infection during bone repair are explored, including bactericidal activity, anti-biofilm, immunomodulation and regenerative properties. Moreover, we present distinctive applications of AMPs for topical bone repair, which can be either used by delivery system (surface immobilization, nanoparticles and hydrogels) or used in gene therapy. Finally, future prospects and ongoing challenges are discussed.

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“…It exhibited excellent antibacterial activity with a series of clinically related bacteria and significantly promoted vascularization and osseointegration in surrounding tissue. Wang et al linearly combined antibacterial peptide (HHC36) and osteogenic peptide tripeptide arginine-glycine-aspartic acid (RGD) [54] to enable a Ti implant modified by polypeptide to possess antibacterial and osteogenic functions [26]. Similarly, Zhang et al prepared hBD3 and RGD peptides and used TBP-1 to attach to the titanium surface [27].…”

Section: Modification Of Implant Surfacesmentioning

confidence: 99%

Functionalization of biomedical materials using fusion peptides for tissue regeneration

Guo

Ruhan

et al. 2022

Biomed. Mater.

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Tissue development deformity or tissue defect is a major clinical challenge. Tissue engineering technology provides a promising solution to these problems. Among them, functional biomaterials with regenerative abilities are one of the development trends. Polypeptide is a small molecule that can be used to modify tissue engineering materials. However, the function of a single polypeptide molecule is limited and insufficient to construct comprehensive microenvironment for tissue regeneration. Fusion peptides combining two or more polypeptide molecules with different functions were expected to achieve multiple efficacies in vivo, providing a novel solution for clinical tissue regeneration engineering applications. This paper reviews the construction methods, degradation process, and biological activities of fusion peptides, and presents recent global research progress and prospects concerning fusion peptides．It provides a reference helping to guide the future exploration and development of fusion peptide-based functional biomaterials for tissue engineering.

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“Dual-functional” strontium titanate nanotubes designed based on fusion peptides simultaneously enhancing anti-infection and osseointegration

Cited by 41 publications

References 59 publications

Antibacterial properties of antimicrobial peptide HHC36 modified polyetheretherketone

Antibacterial properties of antimicrobial peptide HHC36 modified polyetheretherketone

Antimicrobial peptides for bone tissue engineering: Diversity, effects and applications

Functionalization of biomedical materials using fusion peptides for tissue regeneration

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