Dual functional selenium-substituted hydroxyapatite

Abstract

Hydroxyapatite (HA) doped with trace elements has attracted much attention recently owing to its excellent biological functions. Herein, we use a facile co-precipitation method to incorporate selenium into HA by adding sodium selenite during synthesis. The obtained selenium-substituted HA products are needle-like nanoparticles which have size and crystallinity that are similar to those of the pure HA nanoparticles (HANs) when the selenium content is low. HANs are found to have the ability to induce the apoptosis of osteosarcoma cells, and the anti-tumour effects are enhanced after incorporation of selenium. Meanwhile, the nanoparticles can also support the growth of bone marrow stem cells. Furthermore, the flow cytometric results indicate that the apoptosis induction of osteosarcoma cells is caused by the increased reactive oxygen species and decreased mitochondrial membrane potential. These results show that the selenium-substituted HANs are potentially promising bone graft materials in osteosarcoma treatment due to their dual functions of supporting normal cell growth and inducing tumour cell apoptosis.

Cationic and Anionic Substitutions in Hydroxyapatite

“…2À / SeO 3 2À Substitution on Biological Properties of HAp On the basis of strong anticarcinogenic properties of selenium [406,407], already described and discussed, Se-HAps could serve as scaffolds not only for the growth of new bone tissue but also for the inhibition of the tumor cell growth and proliferation, presenting potential applications for treating bone cancers to reduce the recurrence probability.…”

“…Indeed, it is a component of selenoproteins and of the glutathione peroxidase enzyme, responsible for the protection of cell membranes against harmful factors [405]. It is well known that selenium plays a pivotal role in protein functions, and it is involved in the induction of cancer cell apoptosis [406]. Monteil-Rivera et al [407] underlined that selenate (SeO 4 2À ) and selenite (SeO 3 2À ) oxyanions exert their cancer chemopreventive action by direct oxidation of thiol-containing cellular substrates, making it more efficient anticarcinogenic agents than selenomethionine or selenomethylselenocysteine with a lack of oxidation capability.…”

“…Selenium oxyanion-substituted hydroxyapatites (Se-HAps) have been synthesized by a coprecipitation method [406,412,413] with a Se/P molar ratio up to 1.1.…”

Preparation and characterization of selenite substituted hydroxyapatite

“…The incorporation of selenium into apatites seems like a hopeful idea for treating bone cancers to reduce the recurrence probability. Recently nano-crystalline selenite-substituted HA (Se-HA) has been studied for the inhibiting effect on the growth of osteosarcoma cells in our group [19]. Therefore, the role of selenite substitutions in the HA lattice is important with respect to the physicochemical properties and bioactivities.…”

Fe3+/− dual doped nano hydroxyapatite: A novel material for biomedical applications

“…13 Likewise, according to another report Se substitution in HA structure enhanced induction of apoptosis in osteosarcoma cells. 16 Wang et al confirmed that Se-HA materials might be promising as a carrier for biological molecules. 17 Thus, the use of Sedoped HA will increasingly receive interest by researchers for biomedical applications.…”