Dual Functional Capability of Dendritic Cells – Cytokine-Induced Killer Cells in Improving Side Effects of Colorectal Cancer Therapy

Mosińska, Paula; Gabryelska, Agata; Zasada, Malwina; Fichna, Jakub

doi:10.3389/fphar.2017.00126

Cited by 19 publications

(17 citation statements)

References 49 publications

(71 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…CIK are autologous T cells, NK cells and NKT cells activated and expanded ex-vivo under the influence of anti-CD3 stimulation and cytokines. The DC-CIK combined vaccine has been shown to elicit far less adverse events as compared to standard chemotherapy and has shown promising potential in terms of improving over all survival and quality of life when tested in patients [124, 125]. Adoptive transfer of tumor specific T cells or T cells with engineered T cell receptor [126] in combination with TAA loaded DCs is yet another attractive immunotherapy option that has gained momentum due to its anti-tumor effects and usefulness even in advanced tumors [127].…”

Section: Re-emergence Of Dendritic Cell Targeting Vaccinesmentioning

confidence: 99%

Re-Emergence of Dendritic Cell Vaccines for Cancer Treatment

Saxena¹,

Bhardwaj²

2018

Trends in Cancer

227

193

View full text Add to dashboard Cite

Dendritic cells (DCs) are essential in immunity owing to their role in activating T cells, thereby promoting antitumor responses. Tumor cells, however, hijack the immune system, causing T cell exhaustion and DC dysfunction. Tumor-induced T cell exhaustion may be reversed through immune checkpoint blockade (ICB); however, this treatment fails to show clinical benefit in many patients. While ICB serves to reverse T cell exhaustion, DCs are still necessary to prime, activate, and direct the T cells to target tumor cells. In this review we provide a brief overview of DC function, describe mechanisms by which DC functions are disrupted by the tumor microenvironment, and highlight recent developments in DC cancer vaccines.

show abstract

Section: Re-emergence Of Dendritic Cell Targeting Vaccinesmentioning

confidence: 99%

Re-Emergence of Dendritic Cell Vaccines for Cancer Treatment

Saxena¹,

Bhardwaj²

2018

Trends in Cancer

227

193

View full text Add to dashboard Cite

show abstract

“…Bearing the characteristics of CD3+ T lymphocytes, CIK cells are able to identify the antigen presented on DCs 88 . Co-culture of DCs and CIK cells is capable of increasing the expression of CIK-specific characteristics and CIK toxicity on cancer cells 89 . The ratio of DC-CIK co-culture is as diverse as 1: 3, 1: 5, 1:10, and 1:20, according to previous studies 90 91 .…”

Section: Discussionmentioning

confidence: 99%

The priming role of dendritic cells on the cancer cytotoxic effects of cytokine-induced killer cells

Tran

Nguyen

et al. 2019

Sci. Tech. Dev. J.

View full text Add to dashboard Cite

Introduction:In vitro cultivation of DCs and cytokine-induced killer cells (CIK cells) -a special phenotype of T lymphocyte populations -for cancer treatment has gained significant research interest. The goal of this study is to understand whether the priming from DCs helps CIK cells to exert their toxic function and kill the cancer cells. Methods: In this research, DCs were differentiated from mononuclear cells in culture medium supplemented with Granulocyte-macrophage colony-stimulating factor (GM-CSF), and Interleukin-4 (IL-4), and were induced to mature with cancer cell antigens. Umbilical cord blood mononuclear cells were induced into CIK cells by Interferonγ (IFN-γ), anti-CD3 antibody and IL-2. After 4-day exposure (with DC:CIK = 1:10), DCs and CIK cells interacted with each other. Results: Indeed, DCs interacted with and secreted cytokines that stimulated CIK cells to proliferate up to 133.7%. In addition, DC-CIK co-culture also stimulated strong expression of IFN-γ. The analysis of flow cytometry data indicated that DC-CIK co-culture highly expressed Granzyme B (70.47% ± 1.53, 4 times higher than MNCs, twice higher than CIK cells) and CD3+CD56+ markers (13.27% ± 2.73, 13 times higher than MNCs, twice higher than CIK cells). Particularly, DC-CIK co-culture had the most specific lethal effects on cancer cells after 72 hours. Conclusion: In conclusion, co-culture of DCs and CIK cells is capable of increasing the expression of CIK-specific characteristics and CIK toxicity on cancer cells.

show abstract

“…But sometimes, it is very difficult to obtain a sufficient number of CIK cells due to the poor health situation of patients, such as elderly people and patients with immunodeficiency diseases (37). To solve this problem, getting CIK cells from donor PBMC seems to be an alternative option.…”

Section: Ex Vivo Expansion and Alloreactivity Of Cik Cellsmentioning

confidence: 99%

Cytokine-Induced Killer Cells As Pharmacological Tools for Cancer Immunotherapy

et al. 2017

View full text Add to dashboard Cite

Cytokine-induced killer (CIK) cells are a heterogeneous population of effector CD3+CD56+ natural killer T cells, which can be easily expanded in vitro from peripheral blood mononuclear cells. CIK cells work as pharmacological tools for cancer immunotherapy as they exhibit MHC-unrestricted, safe, and effective antitumor activity. Much effort has been made to improve CIK cells cytotoxicity and treatments of CIK cells combined with other antitumor therapies are applied. This review summarizes some strategies, including the combination of CIK with additional cytokines, dendritic cells, check point inhibitors, antibodies, chemotherapeutic agents, nanomedicines, and engineering CIK cells with a chimeric antigen receptor. Furthermore, we briefly sum up the clinical trials on CIK cells and compare the effect of clinical CIK therapy with other immunotherapies. Finally, further research is needed to clarify the pharmacological mechanism of CIK and provide evidence to formulate uniform culturing criteria for CIK expansion.

show abstract

Dual Functional Capability of Dendritic Cells – Cytokine-Induced Killer Cells in Improving Side Effects of Colorectal Cancer Therapy

Cited by 19 publications

References 49 publications

Re-Emergence of Dendritic Cell Vaccines for Cancer Treatment

Re-Emergence of Dendritic Cell Vaccines for Cancer Treatment

The priming role of dendritic cells on the cancer cytotoxic effects of cytokine-induced killer cells

Cytokine-Induced Killer Cells As Pharmacological Tools for Cancer Immunotherapy

Contact Info

Product

Resources

About