Dual Function of Silver Nanoparticles as Matrix Extracell Removal and Antimicrobial Agent in Polymycrobial Biofilms

Abstract

Candida albicans often form polymicrobial biofilms along with pathogenic microbes. Silver nanoparticles (AgNPs) were well known to have strong antimicrobial activity. However, their effect on polymicrobial biofilms and the mechanism has never been reported. This study aimed to synthesize AgNPs and study their effects on polymicrobial biofilm represented by C. albicans–E. coli biofilm. Polymicrobial biofilms, formed by clinical isolates of C. albicans and E. coli, were developed from the standardized suspensions of each strain by culturing flat-bottom 96-well microtiter plates for 48 h, then treated with AgNPs. Cell viability was assessed using the tetrazolium salt reduction assay; the extent of biofilm formation was measured by crystal violet staining. AgNPs reduced the polymicrobial biofilm in two ways: by degrading the extracellular matrix and killing both C. albicans and E. coli. The results showed AgNPs is a potential new approach for developing potent anti-biofilms.

Polymicrobial Infections and Biofilms: Clinical Significance and Eradication Strategies

“…The synthesized silver nanoparticles entered the EPS matrix and degraded the extracellular matrix growth, leading to the destruction of polymicrobial biofilms. Thus, AgNPs showed dual action by degrading EPS matrix and killing the polymicrobial biofilms of E. coli and C. albicans [ 171 ].…”

Recent Advances and Opportunities in the Study of Candida albicans Polymicrobial Biofilms

“…In recent years, the effectiveness of metal nanoparticles against C. albicans PMBs have also been studied. Silver nanoparticles were found to be effective against C. albicans-E. coli (Yasinta et al, 2021) and C. albicans-S. aureus biofilms and could be incorporated into silicone, that may be used for the manufacture of catheters (Lara and Lopez-Ribot, 2020) or maxillofacial prosthesis (Chong et al, 2021). Selenium nanoparticles (stabilized with either chitosan or bovine serum albumin) were tested for their ability to inhibit C. albicans-S. aureus biofilms (Filipovićet al, 2021).…”