“…Polyclonal antibodies against Sec16-N (374–387 aa), Sec16-C (2,319–2,332 aa), and TANGO1-CT (1,884–1,898 aa for TANGO1L; 762–776 aa for TANGO1S) were raised in rabbits by immunization with keyhole limpet hemocyanin-conjugated peptides and affinity-purified by columns conjugated with the peptides (Thermo Fisher Scientific; Iinuma et al, 2007; Saito et al, 2009, 2011; Maeda et al, 2016; Tanabe et al, 2016). Polyclonal antibodies against Sec31 and TANGO1-CC1 were raised in rabbits by immunization with recombinant Sec31 (522-719 aa) or GST-tagged TANGO1L (1,231–1,340 aa) [TANGO1S (109–218 aa)] and affinity-purified by columns conjugated with GST-tagged Sec31 (522-719 aa) or ColdTF-tagged TANGO1 (1,231–1,340 aa) [TANGO1S (109–218 aa)] (Saito et al, 2011; Tanabe et al, 2016). Other antibodies were as follows: CK1δ (rabbit; Proteintech), CK1ε (mouse; BD), PPP1CA (mouse; Santa Cruz), PPP1CB (mouse; Santa Cruz), GAPDH (mouse; Merck), FLAG (mouse; Merck), FLAG (rat; agilent), HA (rat; Roche), and Sec31 (mouse; BD).…”